| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,384,808 | C→T | K305K (AAG→AAA) | yfbK ← | IPR002035/DUF3520 domain‑containing protein YfbK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,384,808 | 0 | C | T | 90.0% | 50.3 / ‑3.7 | 20 | K305K (AAG→AAA) | yfbK | IPR002035/DUF3520 domain‑containing protein YfbK |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (11/7); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTCAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCT‑T‑CGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATC > NC_000913/2384562‑2385042 | aTTCAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATACACCCTT‑t‑cg > 2:118853/1‑251 (MQ=255) tACTTTCCGCGATTCCGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCACCATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACCGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCATT‑T‑CGTCGCct > 2:139559/1‑251 (MQ=255) agagagTGTATCAATGTAGCGGTAGTTGCCGTGACCAACATCGGCAATCCGCACCATCATTGCCTCGTTGTAAGTGCTATTCCCCACGCCAATCGTCGACAGAGTAACACCAGACTCCCGCTGTTTGTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACTTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCTCTTAATAAACCCTT‑T‑CGTCGCCTGCTGAGAATCCAGtt < 2:192788/251‑1 (MQ=255) tACCAACATCGGCAATTCGCCCCAACATTGCCTCGTTTTAATGGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGGCTCCCGCTGTTTTTTGACCATTGATTCAACCAATTTTGGATCGCCAATGCCAACGTTAAAGCCACCGTCAGTGGCTAATAAAATGCGATGGATGCGGCCCTGAATAAACCCTT‑T‑CGTCGCCGTCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTc < 1:384157/250‑1 (MQ=255) cACCATCATTGCCTCGTTGTAATTGCTATCCCCCACGCCAAACGGCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGTTCGTCAATGCCAACGTTAAGGTCACCGTCAGTGGCTAATAAAAGGCGATGGATGCCGCCCTTAATAAACCCTT‑T‑CGTCGCCTGCTTATAAGCCAGTGCCACCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTg < 1:44114/251‑1 (MQ=255) aaTTGTTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGTTGTTTTTTGACCATTGTTTCAATCGATTTTGGATCTTCAATGCCAACTTTAAAGTCCCCGTCAGTGGCTAATAAAAGGCGATGGATGCCGCCCTTAATAAACCCTT‑T‑CGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCtt < 1:227179/250‑1 (MQ=255) tgctaatcaacACGCCAAACGTAGACAGAGAAACACCAGACTCCCGCTGTCTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑T‑CGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGAGGCATTAATTTCCGCCTTATg > 2:383123/11‑250 (MQ=255) cTATTCCCCACGCCAAACGTCGCCAGAGTAACCCCAGACTCACGCTGTTTTTTGACCATTGATTCAATCGATTTTGGCTCATCAATGCCAACGTTAAAGTCAACGTCAGTGGATAATAGAATGCGATTGATGCCGCCCTTAATAAACCCTT‑T‑CGTCGCCGGCTGATCAGCCAGTTCCTGCCCGGTACCGCCATTTGTACTGCCTTCGGCATCCAGCGAATCAATTGCCGCATTAATTTACGCCTTATGACt > 2:79118/1‑251 (MQ=255) cACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATAGATTCAATCGATTCTGGCTCGACAATGCCAACGTTAACGTCACCGTCAGTGGCTAATAACATGCGATTGATGCCGCCCTTACTAAACCCTT‑T‑CGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGACTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCgg > 1:62581/1‑248 (MQ=255) gCCAAACGTCGACAGCGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGCTTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑T‑CGTCGCCTGCTGCTAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGAt > 2:329216/1‑249 (MQ=255) ttttGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCTTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑T‑CGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCAc < 1:63927/250‑1 (MQ=255) ttGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑T‑CGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGAt > 1:179421/1‑251 (MQ=255) aCCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑T‑CGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGc > 1:167463/1‑251 (MQ=255) aTTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑T‑CGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGAACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATATGGGAGTCGCCAGCGTAGGTCACGATAGCAAt > 1:222655/1‑251 (MQ=255) ggatccAATCTATTTTGGTTCGGCAATGCCACCTTTAAAGTCCCCGTCAGTGTCTAATAAAATGCGATTGTTGCCGCTTTTATTAAACCCTT‑C‑CGTCTCCTGCTTATAAGCCGGTCCCTGCCCGGCACCGCCATTGGTACGGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCTCCTTATGACTCCCTGAGATAGAAGGTTATGCAATACCGGAGTCGCCAGCGTAGGTCACGATGGCAATGt < 1:178008/246‑1 (MQ=255) cctaTTTTGGATAGTCAATGCCAACGTTAAAGTACCCGCCAGTTGCTAATAAAATGCAATGGATCCCGCCCTTACTAC‑CCCCTGT‑CGTCGCCTGCTGATAACCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGTCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCTCCAGCGTAGGTAACGATGGCAATGTTATCCttc < 2:93271/248‑3 (MQ=255) gATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑T‑CGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGt < 1:118853/251‑1 (MQ=255) aaaGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTT‑T‑CGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTc > 1:197556/1‑250 (MQ=255) aaGTCACCTTCAGTGGCTAATAAAATGCGATTGATGCCGCCTTGAATAA‑CCGCT‑TGCGCCGCCGGCGTATAAGCCAGTTCCAGCCCGGCGCCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATGGCGGCATTAAGTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGAGGGGAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCaa < 1:165532/251‑1 (MQ=255) gccCTTAATAAACCCTT‑T‑CGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATc > 1:163179/1‑250 (MQ=255) | ATTCAATACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCT‑T‑CGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATAAGTGGCAAACGTTCATCAGAAATC > NC_000913/2384562‑2385042 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |