Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,339,162	C→T	D87N (GAC→AAC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,339,162	0	C	T	97.8%	133.6 / NA	45	D87N (GAC→AAC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/22);  minor base G (1/0);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCAGCAGGTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCT  >  NC_000913/2338918‑2339405                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     aCCAGCAGTTTAGTAATTTGGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACTAAATCGACCGTCTCTTTTTGGAGATTGGCCATTGGTTCATTGGCAATTTTCGCGAGACGTATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGGCGATAGTACCGAAGTTTCCCTGACCGTCTACCAGGTTATGACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGAc                                                                                                                                                                                                                                                <  1:186228/251‑1 (MQ=255)     gcagGTTAGGAATTTTGGTTGGCATGACTCCCGGAATTTTTGCCGGGCCGTCATAGTTATCAACGAAATCCACCGTCGCTTTTTCGAGATCGGCCATCAGTTCAGGGGCAATTTTCTCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAGCCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGAGCGTGTTATAGTccgcc                                                                                                                                                                                                                                            <  1:69873/251‑1 (MQ=255)      cagGTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGAccgccg                                                                                                                                                                                                                                           >  1:215684/1‑251 (MQ=255)      cagGTGAGAAATTTTGGTTGGCATGCCGGCCGGAAGTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCAGATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGAccgccg                                                                                                                                                                                                                                           <  2:168010/251‑1 (MQ=255)         gtgagtagttttGGTGGGCATGTCGGCCGGAATTTGTTCCGTGCCGTCATAGTTATCAACGAAATCGGCCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCGGCGCCATGCGGGCGATCGTGTTATAGACCGCCGAGt                                                                                                                                                                                                                                        <  2:86230/243‑1 (MQ=255)             gtcaTTTTGGTTGGCAGGACGTCCGGAATTTCTTCCGTGCCGTCATAGTTATCAACGAAATCGCCCGTCTCTTTTTCGAGATCGGCCATGATTTCATGGGCAATTTGCGCCAGACGGATTTCCGTAGAACGCATGGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACGGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCAcc                                                                                                                                                                                                                                    <  2:388871/248‑1 (MQ=255)              gAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGAAGATCGTGTTATAGACCGCCGAGTCaac                                                                                                                                                                                                                                    >  2:402020/1‑248 (MQ=255)                    tgtgtggctggACGTCCGGAATTTTTTACGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGTCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTGCCGTATAACGAATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTAACCA‑Tgggg                                                                                                                                                                                                                             <  2:329106/240‑1 (MQ=255)                    tggttggCATGACGTCCGGAATTTTTTCCGAGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGCCGCTCGTGTTATAGACCGCCGAGTCACCA‑Tgggg                                                                                                                                                                                                                             >  2:156404/1‑250 (MQ=255)                         ggCATGACGTCCGGAATTTTTTCCGTTCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGTCCATCAGTTCATGGGCAATTTTCGCCAGACGGAGTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCGGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGt                                                                                                                                                                                                                        <  1:22223/250‑1 (MQ=255)                                   ccGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGAtt                                                                                                                                                                                                             >  1:318755/1‑251 (MQ=255)                                               ccGTGCAGTAATAGTTATCAACGAAATCGACCGTCTCGTGTTCGAGATCGGCAATAAGTGCAGGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATGGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGGCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAAc                                                                                                                                                                                                  <  2:53917/250‑1 (MQ=255)                                                cGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAATGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGa                                                                                                                                                                                                <  1:281138/251‑1 (MQ=255)                                                      gTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATAGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCAGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGGCACAAACGGCACggg                                                                                                                                                                                          >  2:350757/1‑251 (MQ=255)                                                             ttATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGTATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGAtt                                                                                                                                                                                    <  2:153025/250‑1 (MQ=255)                                                               aTCAACGAAATCGACAGTCTCTTTTTCGAGATCGGCCATAAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATACCCCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGCGAATGGCAGCGCCATGCGGACGATCGTGTGCTAGACCGCCGAAT‑ACCACTGGGGAAGGTCTTTACCGCTTACGTCACCAACGACACGGGCAGAtttgt                                                                                                                                                                                 >  2:154997/1‑249 (MQ=255)                                                               aTCAACGAAAGCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGAtttt                                                                                                                                                                                  <  1:131971/250‑1 (MQ=255)                                                                                          gagaTCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCt                                                                                                                                                       >  1:354146/1‑250 (MQ=255)                                                                                          gagaTCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCt                                                                                                                                                       >  1:272279/1‑250 (MQ=255)                                                                                               cGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCCTATAACGCAGCGAGAATGGCTGCGCCCTGCGGGCGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCCGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTCGTACg                                                                                                                                                 >  1:205988/1‑251 (MQ=255)                                                                                                       aGTTCATGGGCAATTTTCGACAGACGGATTTACGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGCAGTTACCCTGAACGTCTACCCGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTCCGTTCATGGc                                                                                                                                         >  2:48459/1‑251 (MQ=255)                                                                                                             ggggaaatttaagcaaGACGGATTGCAGGATATCGCATGGGCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCAACA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTGTTTATAGGCTTTGTTCCAGTCATTGCCTATTACGTTCATGGCGTAAAg                                                                                                                                   <  2:35593/236‑1 (MQ=255)                                                                                                                    ttttCGCCAGGCGGATATCCGTATAACGCATTGCCGCCGCAGAGTCGACGTCGATAGAACCGCAGTTACCATGACCGTCTACCAGCATATAACGCAGCGCGAATGGCTGCGCCATGCGAACGATCGTGTTATAGACAGCCGAGTCACCC‑TGGGGATGGAATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCCTGGCGTAACGTAagcgc                                                                                                                            >  2:49628/1‑250 (MQ=255)                                                                                                                         gCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGgtg                                                                                                                       <  1:267533/251‑1 (MQ=255)                                                                                                                                  aTTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTTACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCAGTGCCTAGTACGTGCATGTCGTAAAGTACGCGTCGGTGTACCGGCtt                                                                                                              <  2:179036/251‑1 (MQ=255)                                                                                                                                       cGTATAAAGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTAAGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGCACGCGACGGTGTAACGGCTTCaagc                                                                                                         >  2:42163/1‑248 (MQ=255)                                                                                                                                                          gcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGc                                                                                        >  1:244528/1‑249 (MQ=255)                                                                                                                                                             gagTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGAATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTCGTACGATCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAacgc                                                                                   >  2:30329/1‑251 (MQ=255)                                                                                                                                                                 cGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACgg                                                                               <  2:379988/251‑1 (MQ=255)                                                                                                                                                                  tccGTCGATAGAACCGCAGTTACCCAGACCGTCTACCAGCATATAACGCAGCGAGTATGGCTGCGCCCTGCGGACGTTCGCGTTACCGACCTCCGAGACACCA‑TGGGGATGGTATTTACCGATTACGTCACCACCGCCACGGTCAGCTTTTTTATAGCTTTTGTTCCAGTCATTTCCTAGTACGTTCATCGCGTATAGTACGCGCCGGTTTACCGCCTTTCGTCAATTTCGGACAACTGGCAgcgcccgtc                                                                              >  2:242892/2‑246 (MQ=255)                                                                                                                                                                                   aGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATc                                                             >  1:44656/1‑251 (MQ=255)                                                                                                                                                                                        cccTGACCGTCTACCAGCATATAACGCATCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATa                                                        <  2:318755/251‑1 (MQ=255)                                                                                                                                                                                        cccTGACCGCCTCCCATCATATAACTCAGCCATAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTTCCCA‑TGAGGATGGTATTTACCGATTACGTCACCACCGACACGTGCAGAGGTTTTATAGGCTTTGGTCCAGTCACTGCCTATTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGTCCATCTTGGTCATCTCGCAGCGCACTGCCAACGATGACCGACATCGCATa                                                        <  2:340451/251‑1 (MQ=255)                                                                                                                                                                                            gACCGTCTCGCAGAATATAACACAGCGAGAATGGCTGCGCCATGAGGACGATCGTGTTATAAACAGCCGAGTCACCC‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCCGTCATTGCCTAGTACGTTCCTGGCGTAAAGTACGCGACGGTGGACCGGCTTCAGGCCATCTCGGACATCTTGCAGCGCACGGCCAACAATGCCCGACATCGCATAATcc                                                    >  2:179154/1‑251 (MQ=255)                                                                                                                                                                                              ccGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAg                                                  <  1:48459/251‑1 (MQ=255)                                                                                                                                                                                                   tACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTAACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAgg                                             >  1:179112/1‑251 (MQ=255)                                                                                                                                                                                                     ccAGCCTATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGAt                                                                                                                                                                                                              >  1:308222/1‑88 (MQ=255)                                                                                                                                                                                                     ccAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGAt                                                                                                                                                                                                              <  2:308222/88‑1 (MQ=255)                                                                                                                                                                                                        gCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctc                                        <  1:279361/251‑1 (MQ=255)                                                                                                                                                                                                                 gCAGCGAGAATGGCTGCTCCATGCTGACTATCGTGGTAGAGTCCGCCGAGTCACCA‑TGGGGATGGGATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGTCTGTGTGCCAGTCATTGCCTAGTACGTTTATTGCGTAAAGTACGCGACTGTGTACCGGCTTCAGGCGATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttcagctct                               <  1:239845/251‑1 (MQ=255)                                                                                                                                                                                                                 gCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGTACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttcagccct                               >  2:37728/1‑248 (MQ=255)                                                                                                                                                                                                                                gcgcCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGAcc                <  1:157874/251‑1 (MQ=255)                                                                                                                                                                                                                                     aTGCGGACGCTCGTGTTATAGCCCGCCGAGTCACCA‑TGGGGATGGCCTTTACCGCTTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGAAGGTGTACCCGCTTCAGCCCATCTCGGACATATGGCAGCGCACGACCCACAATGACCGACATCGCATAATCCCGATAAGAGATCTCCAGTGCTTCCTAACTGTTGACCGgtgg           >  2:173590/1‑250 (MQ=255)                                                                                                                                                                                                                                            cGATCGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTtct    >  1:44980/1‑251 (MQ=255)                                                                                                                                                                                                                                              accGTGTTATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTtctct  >  1:50147/3‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ACCAGCAGGTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCA‑TGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTCT  >  NC_000913/2338918‑2339405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑