Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,329,209	A→G	W197R (TGG→CGG)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,329,209	0	A	G	100.0%	101.6 / NA	35	W197R (TGG→CGG)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/24);  total (11/24)

GTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGG  >  NC_000913/2328971‑2329455                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       gtACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCGGGTGCCACTGCCAGTAACCCACAGCGGTGTTCCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTTTCATCGGCAAACCAGCTCCGGGTGTTCACCCg                                                                                                                                                                                                                                            <  2:257657/251‑1 (MQ=255)                     tttCTTCAGCGGATAGCGGGCAAACAGCTCCCCCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTGCCGAGCATg                                                                                                                                                                                                                         <  2:262331/250‑1 (MQ=255)                            aGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGac                                                                                                                                                                                                                 <  1:31564/251‑1 (MQ=255)                                           aaCAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCTGTACCCGACTACCGAGCATGGAt                                                                                                                                                                                                                      <  1:385049/231‑1 (MQ=255)                                           aaCAGCTCCAACTCTAAACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTCGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCTGTACCCGACTACCGAGCCTGGAt                                                                                                                                                                                                                      >  2:385049/1‑231 (MQ=255)                                               gCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTGCCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTcc                                                                                                                                                                                              <  1:241012/251‑1 (MQ=255)                                                    aCCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcac                                                                                                                                                                                         >  1:151671/1‑251 (MQ=255)                                                            acaTTGCCCACGGGCAATCTGGGTCGGCACTGGCAAAACCCGATTAAGCTCCGTCGCATAACGGGTTAGTACCGTTTTGCTGGGGCCACTGACAGTAACCCACAGCGGTGTACCATCGGGTAACCAGCCAGCAAAACAACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCa                                                                                                                                                                                 <  2:318050/251‑1 (MQ=255)                                                                 gCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAg                                                                                                                                                                            <  1:206518/251‑1 (MQ=255)                                                                  cccACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCATCACCACATCCAGt                                                                                                                                                                               <  1:280357/247‑1 (MQ=255)                                                                   ccACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGAc                                                                                                                                                                          <  1:166993/251‑1 (MQ=255)                                                                   ccACTGGCAACCTGCGGCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGAc                                                                                                                                                                          <  2:261868/251‑1 (MQ=255)                                                                             ccTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCt                                                                                                                                                                <  1:364850/251‑1 (MQ=255)                                                                                           ggCAAAACCCGATTAATCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTTCCCGACTACTGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCggg                                                                                                                                                  <  2:192727/251‑1 (MQ=255)                                                                                           ggCAAAACCCGATTAAGCACCGTCGCAAAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGcgcg                                                                                                                                                    <  2:3933/249‑1 (MQ=255)                                                                                                 aCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGGGCCACTGCCAGTAACCCACATCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCAGTGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGTATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCGTTGTTTTGCGCGGGTAACGt                                                                                                                                            <  2:72436/251‑1 (MQ=255)                                                                                                  cccGATTAAGCACCGTCGCATAACGGGTTAGTTCCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTTCCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCGTTGTTTTGCGCGGGTAACGta                                                                                                                                           <  2:264451/251‑2 (MQ=255)                                                                                                         aaGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATg                                                                                                                                    >  1:67925/1‑251 (MQ=255)                                                                                                         aaGCACCGGCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTACCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCAGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGCCTTCCTGAGCTTTGTTTTGCGCGGGGAACGTCGCCAATg                                                                                                                                    >  2:279384/1‑251 (MQ=255)                                                                                                               cGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCCGTACCCCACAGCGGTGTACCATCGGTTAACCCGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGACTCa                                                                                                                               >  1:123072/1‑250 (MQ=255)                                                                                                                  cGCATAACTGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACCGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCGGACGGATTTCCCGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAACCAagc                                                                                                                            <  1:61448/250‑1 (MQ=255)                                                                                                                          gggTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTGCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCGCGTCCAGCACCACTGCCAGTAAGACTTCCTGAGCTGTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTa                                                                                                                   <  1:185188/251‑1 (MQ=255)                                                                                                                                       ttttcTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCGCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAg                                                                                                       <  1:225912/246‑1 (MQ=255)                                                                                                                                                 ccACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCtt                                                                                            <  2:151671/251‑1 (MQ=255)                                                                                                                                                        cAGTAACCCACCGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGcac                                                                                                                                                                                         >  1:329188/1‑151 (MQ=255)                                                                                                                                                        cAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCCCCCTGACGGAGTTCCTGTGTATCATCGGCAAACCAGCGCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATGTGCGCCTCGTCCAGcac                                                                                                                                                                                         <  2:329188/151‑1 (MQ=255)                                                                                                                                                                    gCGGGGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTGTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCGTCAGCGTAGTCAGAGACGCt                                                                         <  2:2940/251‑1 (MQ=255)                                                                                                                                                                         gtACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCa                                                                    <  2:67925/251‑1 (MQ=255)                                                                                                                                                                                                      accCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGGAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTAtt                                       >  1:286633/1‑251 (MQ=255)                                                                                                                                                                                                                  ttCCTGTTTATCATCGGACAACCAGCTTAGGGATTTCACCCGTACCCGACTCCCGAGCATGGATGCGACACAGATTTTCGCCTAGTCCAGAACCACATCAAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGATTCAGAGTAGTCAGAGACGCTAACCACGCAGTTGCCTGACGCTGTTGCCAGTATTGCCCCAACACAt                           >  2:344255/1‑251 (MQ=255)                                                                                                                                                                                                                       gTTTATCATCGGCAAACCAGCTCAGGGTTTTCACCCGTACCCGACTACCCAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGCTTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGAGCCTGACGCTGTTGCCAGTTTTGCCACCACACATCTg                        >  2:24723/1‑249 (MQ=255)                                                                                                                                                                                                                         ttATCATCGGCAAACCAGCTCCGGGTGTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTgcgc                     <  2:161480/250‑1 (MQ=255)                                                                                                                                                                                                                                         aGCTCCGGGTTTTCATCAGTACCCGAATACCGAGCATGGATGCGACACCGCTTTTCGCCTCGTCCAGCACCACATACAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAAACGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGCCGCTGTTGACAGTATTGCCCCCACACATCTGCGcc                    >  2:261313/1‑235 (MQ=255)                                                                                                                                                                                                                                         aGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGGAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGcc                    <  1:261313/235‑1 (MQ=255)                                                                                                                                                                                                                                           cTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCCCCCATCTGCGCCAATATGCAACCGCTgcgg  >  2:143415/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       GTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCGG  >  NC_000913/2328971‑2329455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑