Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,448	C→T	intergenic (‑575/+834)	gatD ← / ← gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,448	0	C	T	100.0%	101.3 / NA	29	intergenic (‑575/+834)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/16);  total (13/16)

AGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGGTG‑AGTGGGATAAAAATCAGG‑CTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCA  >  NC_000913/2173208‑2173694                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       aggggcgaaaaaGATTCCCCCGATGCACGGCGCCCCCCACCGCCACGAAGAAGCCAATGGTGGCAAGATCGCAAAACGGCAGCACCTA‑ATTACCCGGCACACAAACAGCAATTAAAATGGTG‑AGTGGGATAAAAATCAGG‑CTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATg                                                                                                                                                                                                                                            <  2:18601/240‑1 (MQ=255)          aaagattcccgcgaggcccGGCGACCGCCATCGCCCCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTG‑ATTTCCCGGCCCAAAAACAGCAATTAAAATGGTG‑AGTGGGATAAAAATCAGG‑CTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTaaa                                                                                                                                                                                                                                   <  1:362455/233‑1 (MQ=255)                   catgATGCACGGCGACCGCCATCGCCACAAAAAAACCCATTTTGCCAAAACCGCCAAACGTCAGCACCGG‑TTTCCCCGGCACACAAACAGCAATAAAAATGTGG‑ATTGGGATAAAAATCAGG‑TTTGCCGATTC‑‑CTCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAGCTGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCatg                                                                                                                                                                                                                          <  1:42128/248‑1 (MQ=255)                                  ccGCCATCCCCCCGAAGAAGCAGAGGGGGGCAAAAACGCCAAAGGGCAG‑ACCTGGATTACCCGGCACACAAACAGCAATTAAAAGGGTG‑AGTGGGATAAAAAACAGG‑CTTGCCGA‑ACC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAcc                                                                                                                                                                                                           <  2:212442/251‑1 (MQ=255)                                           ccACGAAGAAGCCGATGGTGGAAAGACCGCAAACCGCCAGCCCCTG‑ATAACCCGGCACAAAAACAGCAAT‑AAAAAGGTGGAGTGGGAAAAAAACCAGG‑CTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt                                                                                                                                                                                                  <  2:117811/251‑1 (MQ=255)                                                   aagCCGATGGTGGCAAGACCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGGTG‑AGTGGGATAAAAATCAGG‑CTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCAt                                                                                                                                                                                          <  1:147292/251‑1 (MQ=255)                                                                      cgcaaaaCGGCAACACCTA‑ATTCCCCGCCACAAAAAAAGCAATTAAAATGGTG‑A‑TGGGGAAAAAAACAGGGCTGGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTAcc                                                                                                                                                                       <  2:295877/247‑1 (MQ=255)                                                                               gcagcaCCTG‑ATTACCCGGTACACAAACAGCAATTAAAATGGTG‑AGTGGGATAAAAATCAGG‑CTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTgg                                                                                                                                                                                                      >  1:202652/1‑211 (MQ=255)                                                                                 agcaCCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGGTG‑AGTGGGATAAAAATCAGG‑CTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCa                                                                                                                                                             >  1:140730/1‑250 (MQ=255)                                                                                               ccGGCACAAAAACAGCAATAAAAATGGTG‑AGTGGGATAAAAATCAGG‑CTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACa                                                                                                                                               <  1:89213/251‑1 (MQ=255)                                                                                                            aGCAAATAAAATGGTG‑AGTGCGATACAACTCAGG‑CTCGCCGCTACCACC‑‑GCCATATCTCCCAGCAGCACCGCCGGATCAAGGCCCATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTAAGCGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTCGCATTAGCAGCATCACTCCCGCCGTTTTTACAGCCAGCTGCACTACACCTTTGACATCGTAACCcgcg                                                                                                                                   >  2:205418/1‑250 (MQ=255)                                                                                                                      aTGGTG‑AGTGGGATAAAAATCAGG‑CTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAACCGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGAt                                                                                                                        >  1:256431/1‑251 (MQ=255)                                                                                                                                                           cccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGCCCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCACCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAAGCAGCCCCATCACAAAACCCACGGTGACAgg                                                                                       <  1:379228/247‑1 (MQ=255)                                                                                                                                                            cccccGCCGTATGCCCCAGCAGCAACGCCGGATCAAGGCCAAGCAGGACCGCCTGACCGCCGAACTTCGCCTGTAAACGACTACTAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTGAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTGTGACATCGTAGCCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGc                                                                                      <  2:256431/250‑1 (MQ=255)                                                                                                                                                               ccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCGTTGACATCGTAACCCTCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCg                                                                                   <  2:58176/251‑1 (MQ=255)                                                                                                                                                                    gTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGAGGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCaa                                                                               >  1:187817/1‑250 (MQ=255)                                                                                                                                                                     tATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGcc                                                                                                                           >  1:107616/1‑205 (MQ=255)                                                                                                                                                                     tATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCGTTGACATCGTAACCCGCGAGGATGcc                                                                                                                           <  2:107616/205‑1 (MQ=255)                                                                                                                                                                                      gCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTg                                                            >  2:247444/1‑251 (MQ=255)                                                                                                                                                                                         ggATCAAGGCCAATCAGGAAATCCTGACCGCCGGACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGGTAAACCATCCATGATGGGTTTCATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTATTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCGCGCCAAATGGACGCAATTTGCGATGaac                                                         >  2:80447/1‑250 (MQ=255)                                                                                                                                                                                          gATCAAGGCCAATCAGGACCTCCTGACCGCCGATCTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCCTTAGCAGCATCACTGCCGCCGTTTTTGCCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTTCGCTGAata                                                        <  1:282272/251‑1 (MQ=255)                                                                                                                                                                                           aTCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTTAatat                                                       <  1:109233/251‑1 (MQ=255)                                                                                                                                                                                              aaGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCa                                                                                >  1:126818/1‑223 (MQ=255)                                                                                                                                                                                                ggCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTCAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGCTGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATAtcgtcg                                                  >  2:209255/1‑251 (MQ=255)                                                                                                                                                                                                ggCCAATCAGGAACTCCTGACCGCCGAACTGCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCTTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATAtcgtcg                                                  <  1:401259/251‑1 (MQ=255)                                                                                                                                                                                                    aaTCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGgcgc                                              >  1:377800/1‑251 (MQ=255)                                                                                                                                                                                                        aGGAACTCATGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGCCCAAATTTGCGCTGAATATCGTCGGCGCTaa                                           >  2:220058/1‑250 (MQ=255)                                                                                                                                                                                                                   aCCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGt                               >  1:100302/1‑251 (MQ=255)                                                                                                                                                                                                                                                gAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCa  <  1:209255/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       AGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTG‑ATTACCCGGCACACAAACAGCAATTAAAATGGTG‑AGTGGGATAAAAATCAGG‑CTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCA  >  NC_000913/2173208‑2173694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑