Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,122,908	G→A	I24I (ATC→ATT)	cpsG ←	phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,122,908	0	G	A	100.0%	96.9 / NA	29	I24I (ATC→ATT)	cpsG	phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/20);  total (9/20)

TTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCGATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCG  >  NC_000913/2122666‑2123132                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 ttATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCttc                                                                                                                                                                                                                          >  1:17521/1‑251 (MQ=255)   ataATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCttcat                                                                                                                                                                                                                        <  2:322961/251‑1 (MQ=255)     aaTCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCttcat                                                                                                                                                                                                                        <  2:4384/249‑1 (MQ=255)     aaTCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCttcat                                                                                                                                                                                                                        >  1:4384/1‑249 (MQ=255)              gATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGAGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGGTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCg                                                                                                                                                                                                             >  1:332187/1‑251 (MQ=255)                    ggctggcGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAAt                                                                                                                                                                                                       <  1:65754/251‑1 (MQ=255)                            gTAACTTCAATGACGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATATCTTCGGTGCCGGCCATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCCGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTACCACAATGGTTTTCCATTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCCTTCAGTTCTTCGCCTAATTTCCcgcg                                                                                                                                                                                               >  2:381980/1‑251 (MQ=255)                                           ccATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAAGTTCCCGCGAATATCATAGGCttt                                                                                                                                                                                <  2:222737/251‑1 (MQ=255)                                                         aTGGAACGTGGCGAAATAGATCGCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCGTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTGGCGGGTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAAtt                                                                                                                                                                  <  1:205681/251‑1 (MQ=255)                                                           ggAACGTGACGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCCCGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTGCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAAtttt                                                                                                                                                                <  2:261096/251‑1 (MQ=255)                                                               cGTGGCGAAATAGATCGCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAATGTTTCGCTGGTGAGGCGGACATCTCCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTGCCCGCGAATAGCATAGGGTTTGAAGCAGGTTAATTTTTTCa                                                                                                                                                            <  2:59186/251‑1 (MQ=255)                                                                     gAAATAGATCTCTTCGGCGCCGGACATACCAATATCCAGCACGCCAACGCCCGCATCCTGTAAACATATCGCCAGCGCCAGTTTTAAGGCTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTCCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCCGGCACTATCTTCATTCAGTTCTTCGCCTAATTTCGCGCGAGTATCATAGGCTTTAAAGCAGGTTACTTTTTTCATATCGt                                                                                                                                                      >  1:131396/1‑251 (MQ=255)                                                                       aaTAGATCTCTTCGGAGCCGGACATACCAATATCCAGCACGTCAAAGCCAGCCTCCTGTAAACCTTTCGCCAGCGCCAGTTATAAGGTTTCGCTGGTGAGGAGGACATCACCGCCTAACCCAATGGTTTTCGGTTTGAGAAATCAACCATAGGCGCGACCAATGCGCCAGCAAATATCTTCATTCAGTTCTCGGCCTAATTTACTCCGAATATCATAGGCTTTAAAAAAGGTTCATTTTTTCATAACGttg                                                                                                                                                    >  1:236304/1‑250 (MQ=255)                                                                                ctTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGACATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTACTTTTTTCATATCGTTACCCTTTTTc                                                                                                                                           >  1:14013/1‑251 (MQ=255)                                                                                  tCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGGGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTGTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCttttt                                                                                                                                            <  1:186343/248‑1 (MQ=255)                                                                                   cGGTGCCGGACATACCAATATCCCGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTGCAg                                                                                                                                         <  2:285172/250‑1 (MQ=255)                                                                                       gCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAGTTTTTTCATATCGTTACCCTTTTTCAGGCAAt                                                                                                                                    <  1:139630/251‑1 (MQ=255)                                                                                        ccGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAAGTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATa                                                                                                                                   <  1:381980/251‑1 (MQ=255)                                                                                                        ccAGCACGTCAACGCCCGCATCCTGTAAACCTTGCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCCCCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGaa                                                                                                                   <  2:317076/251‑1 (MQ=255)                                                                                                          aGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCATGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGc                                                                                                                 <  1:386511/251‑1 (MQ=255)                                                                                                              cGTCAACGCCAGCATCCTGTAATCCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAAGATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCATTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGcc                                                                                                             <  2:384481/251‑1 (MQ=255)                                                                                                               gtccaCGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACAGCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATGCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTGCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATCTTGTCATATCGGTACCCTTTTTCAGGCAATAGTTGGCCCTGTCCGAAGCGGCCa                                                                                                            <  2:339535/247‑1 (MQ=255)                                                                                                               gTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCa                                                                                                            <  1:316772/251‑1 (MQ=255)                                                                                                                                                                  gCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATAGCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATg                                                         <  1:233658/251‑1 (MQ=255)                                                                                                                                                                        gAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGt                                                   >  1:106709/1‑251 (MQ=255)                                                                                                                                                                                                      tttCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAATCGGCCAATTTTATTTTTGTCTTTATTTACACCGCGGTTTCGCATTCAGTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAAc                      <  1:237103/250‑1 (MQ=255)                                                                                                                                                                                                                         ccATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCg  <  2:220304/251‑1 (MQ=255)                                                                                                                                                                                                                           aTAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGaa                        <  2:256332/227‑1 (MQ=255)                                                                                                                                                                                                                           aTAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGaa                        >  1:256332/1‑227 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 TTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCGATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCG  >  NC_000913/2122666‑2123132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑