Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,103,891	G→A	P207S (CCA→TCA)	wbbK ←	putative glycosyltransferase WbbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,103,891	0	G	A	100.0%	24.9 / NA	9	P207S (CCA→TCA)	wbbK	putative glycosyltransferase WbbK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/4);  total (5/4)

CCCATGTTTCTAACCTAGAGGGAAAACAAACTATATCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTT  >  NC_000913/2103651‑2104075                                                                                                                                                                                                                                                 |                                                                                                                                                                                         cccATGTTTCTAACCTAGAGGGAAAACAAACTATATCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAggg                                                                                                                                                                                <  1:340083/251‑1 (MQ=255)              ccTAGAGGGAAAACAAACTATATCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTc                                                                                                                                                                    >  1:16470/1‑250 (MQ=255)                                                                     ttATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACGGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATGTAATAGTGGATTGTGCTGTCAATTTCTTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATTCTCGTGATACAGCAGGGTAAAATATTGTCATCTCATAAGGGTTATTCTTAAATTGAGAATCAGCATCAGTAAGTTGGCtttt                                                                                                               <  1:263775/248‑1 (MQ=255)                                                                                                  aTCCAGGCCTTCTGAACGACTGATCAAATATTTTGCATACGCATTTTCAGTACCTCTGATCGTACGCAGAAATTTACTATAGGATTGTTCTTTAAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTACGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACa                                                                                >  2:379666/1‑250 (MQ=255)                                                                                                                                    gCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATAttt                                                         <  1:33662/239‑1 (MQ=255)                                                                                                                                    gCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATAttt                                                         >  2:33662/1‑239 (MQ=255)                                                                                                                                                 cTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATcc                                  >  2:57772/1‑249 (MQ=255)                                                                                                                                                    tACCACTGCTAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGATCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAAt                             >  2:37378/1‑251 (MQ=255)                                                                                                                                                                                atTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTAgttttt  <  1:68264/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                         CCCATGTTTCTAACCTAGAGGGAAAACAAACTATATCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTT  >  NC_000913/2103651‑2104075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑