Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,086,634	G→A	A143A (GCC→GCT)	plaP ←	putrescine:H(+) symporter PlaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,086,634	0	G	A	100.0%	121.6 / NA	38	A143A (GCC→GCT)	plaP	putrescine:H(+) symporter PlaP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/22);  total (16/22)

TTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAGGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTAAA  >  NC_000913/2086388‑2086873                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                ttcCGACAGGTTGCTGATGCCGTAAAAGCCAGTAAGGGAGCAGCACAGGAAAGTTGCCCAGGTGATCATTGGGATAACATGTTCATCGCCCGACCAGAGTGGACGAGTGCTCGCAAGCGTACCAGCACCTTCACCTTCAAATACGCCATAAACAACCATGCGCCGAATCACCGCGACCAGCACTACATGCAACACAACGATAACATTGTTGAAGTTCGCTCTGGATTTCAGACTACGCAGTTTAAAAGCgg                                                                                                                                                                                                                                             >  2:268161/1‑251 (MQ=255)   ccGACAGGTTGATGCTGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCCTGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTc                                                                                                                                                                                                                                           >  2:160537/1‑251 (MQ=255)                      gTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGGCGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGcca                                                                                                                                                                                                                        <  1:269062/251‑1 (MQ=255)                                  aaaGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTGCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATcc                                                                                                                                                                                                            <  2:322313/251‑1 (MQ=255)                                    agtagaAGCCCAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGAGTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCAc                                                                                                                                                                                                          <  2:375585/248‑1 (MQ=255)                                    aGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCAc                                                                                                                                                                                                          <  1:90625/251‑1 (MQ=255)                                         atgCACAGGACTGTCGCCCCGGTAAGCACCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCCCCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACgatgg                                                                                                                                                                                                     <  2:313854/249‑1 (MQ=255)                                         aaGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACgatgg                                                                                                                                                                                                     <  1:4633/251‑1 (MQ=255)                                          aGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACgatggg                                                                                                                                                                                                    <  2:25711/251‑1 (MQ=255)                                          aGCACAGGATTGTCGCCCCGGTAATCACCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACgatggg                                                                                                                                                                                                    <  1:296949/251‑1 (MQ=255)                                             acaGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACgatgggatg                                                                                                                                                                                                 >  1:339566/1‑251 (MQ=255)                                                 gATTGTCGCCCCGGTAATCATCGGGATAACGTGCGCATCGCCAGACCAGAATGGACGAGTGCGCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAg                                                                                                                                                                                             <  2:141218/251‑1 (MQ=255)                                                     gccGCCCCGGTAATCATCGGAATAACAGGCGCATCGCCAGACCAGAATGTACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAAGACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGGGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGTGATGGAAggta                                                                                                                                                                                          <  2:89189/248‑3 (MQ=255)                                                                     acGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCAACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCaaaa                                                                                                                                                                            <  2:276719/247‑1 (MQ=255)                                                                         gATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACAGCCACGATGGGATGGAAGGCACCAGAGCTTCAAACTAGAttt                                                                                                                                                                     <  2:58709/251‑1 (MQ=255)                                                                            aaCAGGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTGCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGGTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTc                                                                                                                                                                   <  2:299126/250‑1 (MQ=255)                                                                             aCATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGc                                                                                                                                                                 >  1:234067/1‑251 (MQ=255)                                                                                       cGCCAGAGCAGGAGGGACGAGGGCTCGCCAGAGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAAGCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCGCAAACATCCTCGATGGGATGGAAGGCACCAGAGCTGCAAAATAGATTTTCGCCAGTAGattg                                                                                                                                                       <  2:15620/251‑4 (MQ=255)                                                                                           agaccagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCGCCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCGGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGa                                                                                                                                                   <  2:126249/251‑1 (MQ=255)                                                                                                          aGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAAc                                                                                                                                     >  1:115130/1‑250 (MQ=255)                                                                                                          aGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTACAAAAGAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACa                                                                                                                                    <  2:1783/251‑1 (MQ=255)                                                                                                            tGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGa                                                                                                                                                          >  1:8929/1‑227 (MQ=255)                                                                                                            tGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCAACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGa                                                                                                                                                          <  2:8929/227‑1 (MQ=255)                                                                                                                    aGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCg                                                                                                                            >  2:182327/1‑249 (MQ=255)                                                                                                                                   tcaccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCGGCAATACCTGCAACACGACGCTTTCAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCTACCAGCGCCACCTCAAACATCCACGGTGGGATGGAAGGCACCAGAGCTTCaatctaggttt                                                                                                                                                                     >  1:228494/1‑184 (MQ=255)                                                                                                                                   tcaccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTCCAGACTACGCAGGTTAAAAGCGGGCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGTCACCAGGTCTTCAAAATAGAttt                                                                                                                                                                     <  2:228494/193‑1 (MQ=255)                                                                                                                                         tcaAATGCTCCATAAACAACCCTGCCCAGAATCACCTCGATCAGCACTACCTGCAACACGTCGATTACAGTTTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCTCGATGGGATGGAAGGCACCAGAGCTTCAAAAGAGATGGTCGCCAGTAGAATGTTGATCATCGTCGCTAACAGATAGTCGAGCAGAGAAGACCAACccacca                                                                                                      <  2:370803/250‑1 (MQ=255)                                                                                                                                          caAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCAt                                                                                                     >  1:342524/1‑250 (MQ=255)                                                                                                                                               aCGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACCCGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGcc                                                                                               >  1:133519/1‑251 (MQ=255)                                                                                                                                                 gCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCg                                                                                              >  1:152927/1‑250 (MQ=255)                                                                                                                                                        acaacCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCgg                                                                                      >  1:6276/1‑251 (MQ=255)                                                                                                                                                                                  cAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAgtgt                                                            >  1:1579/1‑251 (MQ=255)                                                                                                                                                                                         aCCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCa                                                       <  1:1034/249‑1 (MQ=255)                                                                                                                                                                                                          tACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCTCAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAg                                     <  1:321633/250‑1 (MQ=255)                                                                                                                                                                                                                     aGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGc                         >  1:245318/1‑251 (MQ=255)                                                                                                                                                                                                                         cGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTcc                     >  2:163698/1‑251 (MQ=255)                                                                                                                                                                                                                                        aCTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCg      <  1:213372/251‑1 (MQ=255)                                                                                                                                                                                                                                             gCAGGATAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAACCATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGCTTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTaaa  >  2:361117/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                TTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAGGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTAAA  >  NC_000913/2086388‑2086873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑