Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,994,637	A→G	Y24H (TAC→CAC)	uvrC ←	UvrABC excision nuclease subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,994,637	0	A	G	100.0%	95.7 / NA	29	Y24H (TAC→CAC)	uvrC	UvrABC excision nuclease subunit C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/15);  total (14/15)

AACGTTGTAACGCGGCTGATAGAGTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCAT  >  NC_000913/1994425‑1994882                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aaCGTTGTAACGCGGCTGATAGAGTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTG‑ATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTTGTTACGGTTTTTaa                                                                                                                                                                                                                <  1:69462/251‑1 (MQ=255)         atcgcgGCTGATAGAGTTTGATGTAGTTGTGTTCCAGCAACAGCTCTTCGGTTTCTGTGTGAGGAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATTACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGc                                                                                                                                                                                                          <  1:13201/248‑1 (MQ=255)                        gTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTg                                                                                                                                                                                           >  1:350241/1‑250 (MQ=255)                                 aggtgtgTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGAtctctcac                                                                                                                                                                                  <  2:103248/247‑1 (MQ=255)                                       gtTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGAGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTg                                                                                                                                                                           >  2:209481/1‑251 (MQ=255)                                                aCAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGtct                                                                                                                                                                  <  2:398107/251‑1 (MQ=255)                                                  ggcgcTTCGGGTGCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTcc                                                                                                                                                                <  2:70560/250‑1 (MQ=255)                                                                         aaCCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGGTTTTTCAGGTCTTTAGCTTTGCAGACATAGATAAACGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAAAGCTTTTGCGTCAAACTGATCAATCACTGACTTGATAATGTCTCCGCCTTACACAGACCATGGCGAAt                                                                                                                                         >  1:37098/1‑251 (MQ=255)                                                                                 cATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTGTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGt                                                                                                                                 <  1:10152/251‑1 (MQ=255)                                                                                   tCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTg                                                                                                                                >  1:313119/1‑250 (MQ=255)                                                                                                 cttggCGAACAGCGCTTCGGTTTTGCGCGCAGCGAGGTTGCTACGGAACTAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATc                                                                                                                 >  1:328818/4‑251 (MQ=255)                                                                                                   gggCGACCAGCGCTTCGTTTTTGCTCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGCTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGGCAGCTCAACATc                                                                                                                 <  2:36865/249‑1 (MQ=255)                                                                                                             cgcTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATgtt                                                                                                     >  1:227115/1‑251 (MQ=255)                                                                                                                          cgcgAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAAc                                                                                        <  1:269423/251‑1 (MQ=255)                                                                                                                              aaGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATAc                                                                                    <  1:209481/251‑1 (MQ=255)                                                                                                                                    ggttgtttcGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATACTCGTCCCGCCAGCATCGTGCAGGCGAGAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTTCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTCACTGATCATACGATAGc                                                                              <  2:287299/243‑1 (MQ=255)                                                                                                                                        gCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGtctc                                                                                                                                                                 <  1:343567/164‑1 (MQ=255)                                                                                                                                        gCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGtctc                                                                                                                                                                 >  2:343567/1‑164 (MQ=255)                                                                                                                                           aCGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCt                                                                       >  2:346973/1‑251 (MQ=255)                                                                                                                                                        gAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGGCATAGATAACCGTACCACCAGCATCGTGCATGCGCTAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTc                                                          >  2:176849/1‑251 (MQ=255)                                                                                                                                                                       aGGTCATTCGCTTTGCCGACATCGATAACCGTACCACCAGCATCGTGCATGCGATAACCGCCTGGCTGGCTGGTTACGGTTTTTAACAACGCTTTTGCGTCAAACTGATCCATCACTGACTTGCTAATGTCTCCGCATTACACAGACAATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGCACATACGATAGCGGTCGCTGCTCACCGTTTTCGGACTGAGATTGAGc                                           >  2:258928/1‑251 (MQ=255)                                                                                                                                                                        ggTCTTTCGCTTTGACGACATAGATAACCGTACCACCAGCATCGTGCATGCGTTAAACGCCTGGCTGGCTGGTTACGGGTTTTAAAAACGCCTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCCCCGCATTACACAGACCATGGCGAATTGACAGGTGCTTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGCCTgagaatg                                              >  2:144494/1‑244 (MQ=255)                                                                                                                                                                                     gCCGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGAtctc                             <  1:254772/251‑1 (MQ=255)                                                                                                                                                                                      ccGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCa                                                                                                             <  2:153504/170‑1 (MQ=255)                                                                                                                                                                                      ccGACATAGATAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCa                                                                                                             >  1:153504/1‑170 (MQ=255)                                                                                                                                                                                               aTAACCGTACCACCAGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTc                   <  1:324425/251‑1 (MQ=255)                                                                                                                                                                                                         caccaGCATCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTgg         <  1:7940/251‑1 (MQ=255)                                                                                                                                                                                                                aTCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAACAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGCTCTCATTGACCTTCTGGCCCTTGGGGatcat  >  2:319975/1‑251 (MQ=255)                                                                                                                                                                                                                aTCGTGCATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGAACACTCACTGACTTGATAATGTCTCCGCATTACACAGAACATGGCGAATTGCCAGATGAGACAGCTCAACATCGCCATGAATGTTTAGCTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTAAGATCTCATTGACCTTCTGGCCCTTGGTGgtcat  >  1:27175/1‑251 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      AACGTTGTAACGCGGCTGATAGAGTTTGATGTAGTTGTGTTCCAGCAACAGCGCTTCGGTTTCTGTGTGAGTAACCGTTACATCAATTTGCTGGATCTGGGCGACCAGCGCTTCGGTTTTGCGCGAAGCGAGGTTGCTACGGAAATAGCTGGAAAGCCGTTTTTTCAGGTCTTTCGCTTTGCCGACATAGATAACCGTACCACCAGCATCGTACATGCGATAAACGCCTGGCTGGCTGGTTACGGTTTTTAAAAACGCTTTTGCGTCAAACTGATCACTCACTGACTTGATAATGTCTCCGCATTACACAGACCATGGCGAATTGCCAGGTGAGTCAGCTCAACATCGCCATGAATGTTTAGTTTACTGAACATACGATAGCGGTAGCTGTTCACCGTTTTCGGACTGAGATTGAGCTGTTCTGAGATCTCATTGACCTTCTGGCCCTTGGTGATCAT  >  NC_000913/1994425‑1994882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑