Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,501	C→T	L371L (CTG→TTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,501	0	C	T	98.7%	254.1 / NA	78	L371L (CTG→TTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (38/38);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCG  >  NC_000913/23256‑23740                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 aTTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCGCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGAt                                                                                                                                                                                                                                             <  1:386098/251‑1 (MQ=255)  ttACACCATTCTCGGCACGGGAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGACACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCCCTTATCCGACGTTGGagg                                                                                                                                                                                                                                            >  1:292600/1‑249 (MQ=255)    acacCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGc                                                                                                                                                                                                                                          >  1:79647/1‑251 (MQ=255)         aTTCTCGGCCCGGTAACAGGTGCGGCGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCACTCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGCAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGa                                                                                                                                                                                                                                      >  2:177374/1‑250 (MQ=255)         aTTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTGCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGaa                                                                                                                                                                                                                                     <  1:177374/251‑1 (MQ=255)           tcgcgtcACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTAGGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGGTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCGCGGACGGCACTTATCTGCCGGGCACTTATCCGATGTTGGATGGCGTGAAc                                                                                                                                                                                                                                    <  2:340327/244‑1 (MQ=255)              cGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCGAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGt                                                                                                                                                                                                                                  <  1:93790/249‑1 (MQ=255)               ggCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCGTTACCCAGCCGTTTATGGGCGTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCtt                                                                                                                                                                                                                               <  1:143841/251‑1 (MQ=255)                 cACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTTAGCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGGGAACGTCTTCa                                                                                                                                                                                                                             <  1:352273/251‑1 (MQ=255)                  acggtaccaGGTGCGGAGCTTGAGATGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTACGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGCCGTTGGGTGGCGTGAACGGCTTCa                                                                                                                                                                                                                             >  2:39394/9‑250 (MQ=255)                          aGGTGCGAAACTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTCCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTCTGTGATCGGTCAGAAATACGGACTGGACACCGCTACCCCGGTTGGCCCGGTCGGCACTTATCTGCCGCGCACTTACCCGCCGTTGGATGGCGTGAACGTCTTCAACGCGAACg                                                                                                                                                                                                                    >  2:268022/1‑251 (MQ=255)                           ggTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACg                                                                                                                                                                                                                    >  1:203855/1‑250 (MQ=255)                              gCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACAt                                                                                                                                                                                                                >  2:300504/1‑251 (MQ=255)                              gCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGGTACCCTGGATGCCGGTTCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACAt                                                                                                                                                                                                                <  1:216914/251‑1 (MQ=255)                                  aacTTGAGCTGCTGCGCTTTCACCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCACGGCGATAAAGTTACCCTGAATGCCGGTACCGGTGCCGATCCCACCGAGCCTGGCCACGGCCCGGACGACTATGTGACCGGTCAGCCATACGCACGGCAAACCGCTAACCCGGGTGGCCCGGACGGCACTTATCTGCCGGGCACGCAACCCACGCTGGACGACGTGCACGTTTACAAAGCGTAAGACAtcgtc                                                                                                                                                                                                            >  2:232520/3‑251 (MQ=255)                                  aGCTTGAGCTGCTGCTCTTTACCCAGCTGTTTCTGGGCCTCGACTTTCCGGCAATCCTCGGCGATCACGTGTCCCTGGCTGCCGGTACCGGTGCCGTTCACCCCGCGCTTGTCCACGGCCCGGACGACTATGTGATCGGGCAGAAATACGGCCTGGAAACCGATAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGCCGTTGGATGGCGTGAACGTCTGCAAAGCGAACGACAtcgta                                                                                                                                                                                                            <  2:225213/251‑2 (MQ=255)                                      tGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGACATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGGTGGCGGGAACGTCTTCAAAGCGAACGACATCGTCGTTg                                                                                                                                                                                                        >  2:135243/1‑251 (MQ=255)                                      tGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTg                                                                                                                                                                                                        >  1:145490/1‑251 (MQ=255)                                           tgctgcGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCgctg                                                                                                                                                                                                   >  1:281536/1‑251 (MQ=255)                                                cgcTTTACCCCTCCGTTTATCGGCTTCGAGGTTCCGGCAATCCGCGGCGATCACGTTACCCTGGATGCCAGTACCGGTGCCGTTCACGCCGCGCCTGGCCGCGGCCCGGACGACTATGTGGTCGGTCAGAAATACGGCCGGGAATCCGGTAACCCGGGTTGCCGGGACGGCACTTAGCTGCCGTTCACTTATCCGACGTCGTATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCa                                                                                                                                                                                              <  1:265159/251‑1 (MQ=255)                                                cgcTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGGCGTTGCGCTGCTGCa                                                                                                                                                                                              <  1:265903/251‑1 (MQ=255)                                                 gcTTTCCCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAg                                                                                                                                                                                             <  2:25468/251‑1 (MQ=255)                                                 gcTTTACCCATCCGTTTATGGGCTTCGACGCTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGCTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGCACGACATCGTCGTTGCGCtgctgc                                                                                                                                                                                               >  2:78623/1‑249 (MQ=255)                                                              gTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGgcgcgc                                                                                                                                                                                >  1:58932/1‑251 (MQ=255)                                                                           gACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGAGCCGTTCACACAGCGCCTGGCCACGGCCCGGACGCCTATGTGATCGGTCAGACATCCGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTCTCCGACGTTGGATGGGGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAACGGCGCGCTGCTGCAAGTTgg                                                                                                                                                                   >  2:261752/1‑250 (MQ=255)                                                                             cGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGGACCGGTGCTGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTg‑aga                                                                                                                                                                <  1:53746/251‑1 (MQ=255)                                                                                   ggCAATCCTCGGCGATCACGTTAACCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGACAAAGGCGCGctgctgaaggtggggaaaagg                                                                                                                                                            >  1:88551/1‑235 (MQ=255)                                                                                         ccTCGGCGGTCCCGTTACCCTGGATGACGGTACCGGGGCCGTTCACCCCGCGCCGGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATTCGGCCTGGAAACCGCTAACCCGGTGGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGcaca                                                                                                                                                    <  2:34986/251‑1 (MQ=255)                                                                                         ccTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGcaca                                                                                                                                                    <  1:365218/251‑1 (MQ=255)                                                                                            cGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTGCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAg                                                                                                                                                   <  1:263561/249‑1 (MQ=255)                                                                                            cGGCGATCACGCTACGCTGACTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCACCGCCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAAACCGGTTGGCCTGGTCCGCCCTTTTCTGCCGTGCACTTATCCGACGTTGGTTGGAGTGAACGTCTTCAAAGCGAACGACATCGTCGTCGCGCTGCCGCTGGGTAAAGGCGCCCTCCTGCTCGCTG‑TGAAAATGCAAcacacct                                                                                                                                                 >  2:149793/1‑248 (MQ=255)                                                                                             ggCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTa                                                                                                                                                <  1:78623/251‑1 (MQ=255)                                                                                              gCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTAt                                                                                                                                               >  2:112258/1‑251 (MQ=255)                                                                                                gATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCtgctgc                                                                                                                                                                          >  1:341896/1‑223 (MQ=255)                                                                                                gATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCtgctgc                                                                                                                                                                          <  2:341896/223‑1 (MQ=255)                                                                                                     cGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGGGTGAACGTCTTCAAAGCGAACGACAGCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGtgct                                                                                                                                        <  1:261752/251‑1 (MQ=255)                                                                                                     cGTTACCCTGGATACCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAACCCGCTCACCCGGTTGGCCCGGACGGCACTTATCTGACGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTAAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGtgct                                                                                                                                        >  2:363696/1‑251 (MQ=255)                                                                                                      gtcacgcTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGCCGAATATGTGATCGGTCAGAAATACGGCCTGGACACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTCTCCGACGTTGGACGGCGTGACTGACTTCACAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGAACGCTGTGGAAAAT‑CCGCACAGCTATCCGtgc                                                                                                                                         >  2:166317/7‑249 (MQ=255)                                                                                                                   ccGGTTCCGGTGCCGGTAACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAGCCTCTAGCCCGGTTGGCCCGGACGGCACGTATCTGCCGGGCACTTAGCCGACGTTGGAGGGCGTGAACGTCGTCAGAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTCG‑AGAAAATGGAGGACAGCTAGCCGGGCTGCTGGCggaacaaa                                                                                                                          <  2:191794/251‑8 (MQ=255)                                                                                                                         ccGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCg                                                                                                                    <  1:36307/251‑1 (MQ=255)                                                                                                                           ggTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGat                                                                                                                  <  1:368694/251‑1 (MQ=255)                                                                                                                               ccGTGCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGatcatc                                                                                                              <  2:281536/251‑1 (MQ=255)                                                                                                                                cGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCt                                                                                                             <  2:69215/251‑1 (MQ=255)                                                                                                                                 gTTCACAACGGGCCTGGCCACGGCACGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGAAGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTAAAAGCGAACGACATCGTCGTGGCGCCGATGCTGGAAAAAGGCGCGCTACTGCACGTTG‑ACAAAATGCAGCACAGCTAACAGTGCTGCTGGCGTCAAAAAACGACGATCATCtt                                                                                                            >  2:339758/1‑251 (MQ=255)                                                                                                                                    cacaCCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCTCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGGTGCTGGCGTCACAAAACGCCGATCATCTTCcg                                                                                                         <  1:337030/251‑1 (MQ=255)                                                                                                                                       acCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACTCCGATCATCTTCcgcgc                                                                                                      >  1:227342/1‑251 (MQ=255)                                                                                                                                        ccGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCcgcgcg                                                                                                     <  1:346285/251‑1 (MQ=255)                                                                                                                                                       gCCCGGAAGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTc                                                                                      <  2:6232/251‑1 (MQ=255)                                                                                                                                                         ccGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGATGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGAGCGACGCCGCAGAGGTTCg                                                                                     >  1:252649/1‑250 (MQ=255)                                                                                                                                                           ggACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTc                                                                                   >  2:304538/1‑250 (MQ=255)                                                                                                                                                           ggACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGAGGGCGTGAACGTCTTCAAAGCGAACGACATCGTCTTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAACACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCa                                                                                  <  1:363696/251‑1 (MQ=255)                                                                                                                                                            gacgacTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGAGAACGACATCGTAGTTGCGCTGCTGCCGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGGGTCACAAAACGCCGATCATCTTCCGCGCGAAGCCGCAGTGGTTCGTc                                                                                   >  1:33328/1‑249 (MQ=255)                                                                                                                                                                   aTGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGtgct                                                                                                                                        >  1:223448/1‑189 (MQ=255)                                                                                                                                                                   aTGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGtgct                                                                                                                                        <  2:223448/189‑1 (MQ=255)                                                                                                                                                                      tgATCGGTCAGAAATACGGCCTGGAACCCGCTAACCCTGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGACCGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAAGGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCa                                                                        <  2:214578/250‑1 (MQ=255)                                                                                                                                                                           ggTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAgg                                                                  <  1:130549/251‑1 (MQ=255)                                                                                                                                                                               aGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCtg                                                              >  2:138244/1‑251 (MQ=255)                                                                                                                                                                                gAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCtgc                                                             >  1:126774/1‑251 (MQ=255)                                                                                                                                                                                  aaTACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCtgcgt                                                           >  2:92950/1‑251 (MQ=255)                                                                                                                                                                                    tACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCtgcgtgc                                                         >  1:92046/1‑251 (MQ=255)                                                                                                                                                                                        gCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTgcgc                                                       >  1:219407/1‑249 (MQ=255)                                                                                                                                                                                          cTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAGAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCCGTc                                                   >  2:156364/1‑251 (MQ=255)                                                                                                                                                                                                aCCGCTACCCCGGTTGGCCCGGATGGCACTTATCTGCCGGGCACTTATCCGTCGGTGGATGGCGTGAACGGCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGTCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCTACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGTTCtgcgtgcaatagatattgc                                             <  2:249201/251‑13 (MQ=255)                                                                                                                                                                                                aCCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGaa                                             <  1:173015/251‑1 (MQ=255)                                                                                                                                                                                                      aaCCCGGGTGGCCCGGACGTCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGCGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGATGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAgaga                                        <  1:309121/250‑1 (MQ=255)                                                                                                                                                                                                       aCCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATc                                      <  1:2229/251‑1 (MQ=255)                                                                                                                                                                                                          cGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAACGAGATCaa                                    >  1:34363/1‑250 (MQ=255)                                                                                                                                                                                                                       aggCACTTAACTGCCGGGATCTTATCCGACGTTGGATGGCGTGAACGTCTTCAGAGCTAACGACAGCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGGTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGa                      <  2:316651/250‑1 (MQ=255)                                                                                                                                                                                                                         gCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATc                    <  1:304538/251‑1 (MQ=255)                                                                                                                                                                                                                         gCACTTATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATc                    <  1:110390/251‑1 (MQ=255)                                                                                                                                                                                                                             ttATCTGCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCgg                >  1:306632/1‑251 (MQ=255)                                                                                                                                                                                                                                   gCCGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTggg          >  1:279635/1‑251 (MQ=255)                                                                                                                                                                                                                                     cGGGCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGCTCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGc        >  1:43199/1‑251 (MQ=255)                                                                                                                                                                                                                                      gggCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGcc       <  1:39394/251‑1 (MQ=255)                                                                                                                                                                                                                                      gggCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGcc       >  2:8547/1‑251 (MQ=255)                                                                                                                                                                                                                                       ggaaaatATCCGACGTTAGATGGCGTGAACGTATTCCAAGCGCACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGCCCCACTGCTGCAAGTTG‑AGAAAATGCAGCACAGCTATCCGAGCTGCTGGCGACACAACACGCCGAACCTCTTCCGCGCTACGCAGCAGTGGATCGCCAGCCTGGCTCAGAAATGTCTGCGTGCGAAGTAACTGAACGAGATCAAAGGAGTGCAGAGGATCCCggcctcaggccc      >  2:382904/7‑241 (MQ=255)                                                                                                                                                                                                                                        gCACTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAg     <  1:300504/251‑1 (MQ=255)                                                                                                                                                                                                                                           cTTATCCGACGTTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGgcg  >  1:46166/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 ATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGTTGCGCTGCTGCAGGAAAAAGGCGCGCTGCTGCACGTTG‑AGAAAATGCAGCACAGCTATCCGTGCTGCTGGCGTCACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCG  >  NC_000913/23256‑23740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑