Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,100	G→A	E172K (GAA→AAA)	ydiF →	putative acetate‑CoA transferase YdiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,100	0	G	A	100.0%	55.7 / NA	17	E172K (GAA→AAA)	ydiF	putative acetate‑CoA transferase YdiF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/8);  total (9/8)

CACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATAT  >  NC_000913/1775869‑1776332                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         cACTGGGGATAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGTTTATACCTACCCACAAGGTGTACTTACACCACCCTTACGCTCCGCCGCAGCTCACCAGCCTGGTCTTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACGGAATGAAGTCACTAAATAAGTCCTGATTAAACTGGTCGAGTTTGATAACAAAAAACATCTCTATTACATAGc                                                                                                                                                                                                                       <  1:14649/251‑1 (MQ=255)                 cgcgTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATAtt                                                                                                                                                                                                       <  1:129339/251‑1 (MQ=255)                      aTTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCtt                                                                                                                                                                                                  <  1:125757/251‑1 (MQ=255)                         tCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTGACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTTAATGAAGTCACTAAAGTAGGCCTGATGAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCa                                                                                                                                                                                                <  1:355445/250‑1 (MQ=255)                                                                 aCCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGATTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACtttt                                                                                                                                                       >  1:174505/1‑251 (MQ=255)                                                                    cacaAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGAATTATTAGTGATATTCGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGCGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGAGAAGGCTACGCCACTTTTGa                                                                                                                                                     >  1:188897/1‑250 (MQ=255)                                                                                                             caccaGCCTGGTATTATTAGGGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGCTGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGc                                                                                                           >  2:146681/1‑251 (MQ=255)                                                                                                                                           aTCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTgatgat                                                                             >  1:140905/1‑251 (MQ=255)                                                                                                                                              gggACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAtt                                                                                                                                                                                              <  1:12137/135‑1 (MQ=255)                                                                                                                                              gggACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCAtt                                                                                                                                                                                              >  2:12137/1‑135 (MQ=255)                                                                                                                                                   aTTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTTCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGc                                                                     <  2:297716/251‑1 (MQ=255)                                                                                                                                                               aCGCCAGCAAGGCGGCAAACTGAATGAAGTCACGAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTTCCTTCATGCGCGCTACCGCCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGATGTGATGTATCTCGACGTATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTa                                                         <  1:229109/251‑1 (MQ=255)                                                                                                                                                                         ggcggcAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTTGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGAGATGTCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCCCTTTTGAAGATGAGGTGATGGATCTCGACGCATTGGTTATTGCTCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCAc                                               <  2:21316/251‑1 (MQ=255)                                                                                                                                                                                        gaaGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACACTAACGGCGGTATTGTGATGATGCAGGTGCAGGAAAAGGTTAAGAAAACCACGCTGCATCCTAAATc                                >  1:273572/1‑251 (MQ=255)                                                                                                                                                                                               cTAAAGTAGAACTGATTAAACTGGTCGAGTTTGCTCACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGCAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTTTTTTGGTGATGTCGGGGGAGAAAATTGTTGAGAAAAACACGCAGCAACCTACATTTGTcc                           >  2:58052/1‑249 (MQ=255)                                                                                                                                                                                                                    tGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAAAGGTTAAGAAAACCACGCTGCATCCTCAATCTGTCCGTATTCCGGGGTATCtggggggt    >  1:56596/1‑246 (MQ=255)                                                                                                                                                                                                                      gTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGATGGTGGGGGGGCAGGAAATGGTTAAGAAAACCACGCCGCATACTAAATCTGTCCGCATTTCGGGGTTTCTGGTGGgtat  >  1:166007/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         CACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATAT  >  NC_000913/1775869‑1776332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑