Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,721,136	C→T	L38F (CTT→TTT)	ydhI →	DUF1656 domain‑containing protein YdhI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,721,136	0	C	T	100.0%	94.9 / NA	29	L38F (CTT→TTT)	ydhI	DUF1656 domain‑containing protein YdhI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/11);  total (18/11)

GTATTGCTAAAACAAATGTATTGCTGCATTTGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGCTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCG  >  NC_000913/1720889‑1721372                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             gTATTGCTAAAACAAATGTATTGCTGCATTTGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTGTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTAT‑CTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTGCAAAGCATTCGCGTGTGGATACGTCATCTGGTTTg                                                                                                                                                                                                                                           <  2:19955/250‑1 (MQ=255)                 tGTATTGCTGCATTGGGTTACCGACAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAACGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGACCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTACGATTTTCAAAGCATTCGCGTTTGGATTCGTGTTCTGGTTTGTGGTACACCGCCTGCt                                                                                                                                                                                                                           >  2:297592/1‑251 (MQ=255)                         tgcATTTGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCt                                                                                                                                                                                                                   >  1:147299/1‑251 (MQ=255)                             tttGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGAt                                                                                                                                                                                                               >  1:213912/1‑251 (MQ=255)                                   tcccGTCAGAAATAATTTTCAGAAATTGCGCGTATATTTTTCGCACTTAAAGAATATTTATTAAACTAACGCAATATATTCGTTCATAAAGGAACCTACTTTGTGAAGTTTATTCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACGTTCCGCCGTTTTTCAAAGCCTTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACg                                                                                                                                                                                                          <  2:314506/248‑1 (MQ=255)                                         cAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCATCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCTTCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGCTCTACGCCGGTg                                                                                                                                                                                                    >  2:213877/1‑250 (MQ=255)                                              atatTTTTCAGAAATTGCGCGTAAATTTTTCGCACATAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTGCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCt                                                                                                                                                                                              <  2:213912/251‑1 (MQ=255)                                                      cAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTACAGGAATCTACTTTGTGAGGTTTATGCTCAATGCAACAGGATTGCCCTGACGAGATCTGGTGTTCGTTGCGTCAGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATccc                                                                                                                                                                                      >  1:397487/1‑251 (MQ=255)                                                          aaTTGCGCGTAAATTTTTCGCACGTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTTGCATCCCTTGt                                                                                                                                                                                  <  2:242643/251‑1 (MQ=255)                                                           aTTGCGCGTTAATTTTTAGCACTTAAAGAATATTTATTAATCTAACGCAATCTATTCGGTCGTAAAGGAATCTCCTTTGTGAAGCTTATGCTCAATGCAAAAGGATTGCCCTTCCAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGtt                                                                                                                                                                                 >  2:392024/1‑251 (MQ=255)                                                                                                   tatAACGCAATATATTCGGTCGTAAAGGAATCTAATTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGttt                                                                                                                                                              >  2:231532/3‑230 (MQ=255)                                                                                                   tCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGttt                                                                                                                                                              <  1:231532/230‑1 (MQ=255)                                                                                                       aCGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTAGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGTCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTgg                                                                                                                                     >  1:40576/1‑251 (MQ=255)                                                                                                              atatTCGGTCGTAAAGGAATCTACTTTGTGCAGTTTATGCTCAATGCAACAGGATTGCACTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACt                                                                                                                              >  2:343403/1‑251 (MQ=255)                                                                                                              atatTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCAGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGAGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGTTTTGCGTTTGCCTTGCTCTGGCAATACt                                                                                                                              >  1:142000/1‑251 (MQ=255)                                                                                                                   cGGTCGTAAAGGAATCTACGTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTGTCCTTCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACGCCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTAGCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGAtt                                                                                                                          <  1:193454/250‑1 (MQ=255)                                                                                                                     gTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTAAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCg                                                                                                                       >  2:253993/1‑251 (MQ=255)                                                                                                                            aGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCTGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGTTTCGTCATCTGGTTTGTCGTTTACCGCCTGCTTCGTGGCTGGATCTACGTCGGTGACTTCTGGCATCCCTTGTTATTGGATTTATCGCTGTTTGCGATTTGCGTTCGCCTTTCTCTGGCAATACTGATTGCGTgttatc                                                                                                                >  2:98377/1‑246 (MQ=255)                                                                                                                                           gAAGTTTATGCGCAATGCAACAGGATTGCCCTTACAAGAGCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCTTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACAGCTGGCATCCCTTGTGAAGGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAAc                                                                                                 <  1:392024/251‑1 (MQ=255)                                                                                                                                                ttATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAAtt                                                                                             <  1:253993/250‑1 (MQ=255)                                                                                                                                                        aaTGCAACATGATTGCCTTTAGAAGATTTTGTGTTCGTTGCGTCCGTCTCCTTTCCGCTGTTTTTCAAAGAATTCGCGTTTTGATTCGTAATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTAGCTCTGGCAATACTGATTGCGTGGTAAATATGTCAATTAACACAATTACGTAtgta                                                                                    <  2:311254/251‑4 (MQ=255)                                                                                                                                                              aaaGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTATcaac                                                                               >  2:95354/3‑250 (MQ=255)                                                                                                                                                               cAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAACGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTcaaca                                                                              >  2:69534/1‑250 (MQ=255)                                                                                                                                                                                          tCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGGAGTTGCGGTTCtt                                                   <  1:316016/250‑1 (MQ=255)                                                                                                                                                                                                              cctccGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCTGGTGACATCTGGCATCCCTTGTTACTGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTACAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGGGGCTGTGGAat                              >  2:120926/1‑251 (MQ=255)                                                                                                                                                                                                                      tttCAAAGCATTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACAt                      >  1:336204/1‑251 (MQ=255)                                                                                                                                                                                                                               aTTCGCGTTTGGATTCGTCATCTGGTTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTcc             >  1:219836/1‑251 (MQ=255)                                                                                                                                                                                                                                         ggATTCGTCATCTTGTTTTTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATTCTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACAcgc   <  2:291022/251‑1 (MQ=255)                                                                                                                                                                                                                                           aTTCGTCATCTGGTTTCTCGTACACCGCCTGCTTCGTGGCTGACTCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACAcgcg  >  1:148474/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                             GTATTGCTAAAACAAATGTATTGCTGCATTTGGTTACCGTCAGACATATTTTTCAGAAATTGCGCGTAAATTTTTCGCACTTAAAGAATATTTATTAATCTAACGCAATATATTCGGTCGTAAAGGAATCTACTTTGTGAAGTTTATGCTCAATGCAACAGGATTGCCCTTACAAGATCTGGTGTTCGGTGCGTCCGTCTACTTTCCTCCGTTTTTCAAAGCATTCGCGTTTGGATTCGTCATCTGGCTTGTCGTACACCGCCTGCTTCGTGGCTGGATCTACGCCGGTGACATCTGGCATCCCTTGTTAATGGATTTATCGCTGTTTGCGATTTGCGTTTGCCTTGCTCTGGCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCG  >  NC_000913/1720889‑1721372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑