Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 3,368,685 IS2 (–) +5 bp coding (859‑863/1128 nt) yhcG → DUF1016 domain‑containing protein YhcG

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3186096 =NA (NA)28 (0.740) 24/486 0.5 100% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 3368685 = 0 (0.000)coding (859/1128 nt) yhcG DUF1016 domain‑containing protein YhcG
* ? NC_000913 = 3187426NA (NA)47 (1.240) 43/488 0.1 97.9% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 3368689 1 (0.030)coding (863/1128 nt) yhcG DUF1016 domain‑containing protein YhcG

TTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTC  >  NC_000913/3185846‑3186330
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                          
ttCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGCATGGAAAACACAAGCAAAGATGACACCCCGGGCAACGTTTACGCTCAGGATGACTAAACGATTGCTTACGAGTAATCGCATTTGGCAGGGTTCACCCGGCCACATCATTAACGGCTTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATACGCGAATTGATTCAAACTATTTCTTGTTCATTATCCGttatt                                                                                                                                                                                                                                            >  1:243259/1‑251 (MQ=255)
                   atgaGGAACTGAATTTCAAAGCTCGTATGGAAGCCCCAAGCAAAGATGCCCCCCCGGGCAGCGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTATCCACAATAAGCGATTTGATTAAAAATATTGATTTTTCATTATCCGGTACTAGACTGGccctcttaat                                                                                                                                                                                                                           <  2:281383/246‑8 (MQ=255)
                                  tCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGACTCTCCAGACAACCAatat                                                                                                                                                                                                          >  2:251943/1‑251 (MQ=255)
                                           atATGTAATACCCAAGCAAAAAAGCCACCACGGTTAACGTTTACGCTCAGGCTGTGTGCAAGATTGTTTACGAGTACTCGCAGTTGTACGGGTTCGCCAGGCCACATCATTAACGGATTAATGATAAGTGGAACAGATGTATAAAAAATTAAAAGTACCCACAATAAGCGAAATGATGAAAAACATTTAATGTCCATTATCCGTTATTAGACTTGCCATCTGAATCTTCAGACAACCATTATCACTTAAAt                                                                                                                                                                                                 <  2:388652/250‑1 (MQ=255)
                                                gAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATAGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACAAATATCACTTAAATAAGTg                                                                                                                                                                                            >  1:384237/1‑251 (MQ=255)
                                                 aaTCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAAGCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGa                                                                                                                                                                                           <  1:176332/251‑1 (MQ=255)
                                                                        ccGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGACTTAATACTAGttttt                                                                                                                                                                       >  1:328763/1‑248 (MQ=255)
                                                                                                                aCGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCg                                                                                                                             <  1:222184/250‑1 (MQ=255)
                                                                                                                      aaTCGCATTTGGCCGGGTACGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGAAGTATAAAAAATTAAAATTAACCACTATAAGCGAATTTATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACCTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAAc                                                                                                                      <  1:396439/251‑1 (MQ=255)
                                                                                                                                   cGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTAc                                                                                                         <  1:221414/251‑1 (MQ=255)
                                                                                                                                                             ggattcaggATAAGTGGATTCGATGTATAAAAACTTAAACCTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTACAGACAACCAATATCACTTAAATAAGTGATAGTATTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTc                                                                               >  2:202290/9‑251 (MQ=18)
                                                                                                                                                                            ggATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCTCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCTGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGaa                                                                 <  2:43464/250‑1 (MQ=255)
                                                                                                                                                                               tCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACAACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCgg                                                             >  2:296607/1‑251 (MQ=255)
                                                                                                                                                                                                   aattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGtt                                         >  2:150716/1‑251 (MQ=255)
                                                                                                                                                                                                             aaTAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGACAAc                               >  1:209446/1‑251 (MQ=255)
                                                                                                                                                                                                                       ttGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCa                                                                                                                   >  2:213746/1‑157 (MQ=255)
                                                                                                                                                                                                                       ttGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCa                                                                                                                   <  1:213746/157‑1 (MQ=255)
                                                                                                                                                                                                                              aaaaTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGACAACATGGTGTAGcagccagc              <  1:322406/251‑1 (MQ=255)
                                                                                                                                                                                                                                    tttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGCTCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGACAACATGGTGTAGCAGCCAGCCAGtt         >  2:215138/1‑250 (MQ=255)
                                                                                                                                                                                                                                       attGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGACAACATGGTGTAGCAGCCAGCCAGTTAttt     <  1:83195/251‑1 (MQ=38)
                                                                                                                                                                                                                                         tGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGGTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTGGCCCGACAACATGGTGTAGCAGCCAGCCAGTTATTtc    <  2:328763/250‑1 (MQ=255)
                                                                                                                                                                                                                                          gTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGACAACATGGTGTAGCAGCCAGCCAGTTATTtctc  <  1:319103/251‑1 (MQ=25)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                          
TTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTC  >  NC_000913/3185846‑3186330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.