Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	1,679,563	(TATCACGTTTTAATCACTGGATATCGATGGAAA)1→2	coding (7/1383 nt)	ydgI →	putative arginine:ornithine antiporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1679531 =	5 (0.130)	23 (0.610)	21/488	0.6	85.2%	intergenic (+160/‑26)	ydgH/ydgI	DUF1471 domain‑containing protein YdgH/putative arginine:ornithine antiporter
	?	NC_000913	= 1679563	3 (0.080)					coding (7/1383 nt)	ydgI	putative arginine:ornithine antiporter

CCGTCAAGTTCGGGACGAATGCGCGTGAGGATCAGCATGGCAAAGGCCAGCAATAAAATGCCAGCGCCAGTAATACCCCAGCCGATGAGCAGTGCTGCCGGGCTGGCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1679762‑1679531 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGCGGGATTTTACGAAGAGATGTGTCTGCATGCAATGGTCGCGGGGAAATTTGTGACTAAAAATGCGAAAGGCGGCATCAAGCCGCCTTATAGGAGTAACTGAATAGTTATTTATACAGATCTGCGCTGACGGTCAGGTTATTACCACGTTCCTGCCACTGGC  <  NC_000913/1679563‑1679316                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  ccgcaAAGTTCGGGACGAATGCGCGTGAGGACCAGCATGGCAAAGGCCAGAAATAAAATGCCAGCGCCAGTAATCCCCCAGCCGATGAGAAGTGCTGCCGGGCGGGCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTG                                                                                                                                                                                                                                       <  2:6189/246‑1   GTCAAGTTCGGGACGAATGCGCGTGAGGATCAGCATGGCAAAGGCCAGCAATAAAATGCCAGCGCCAGTAATACCCCAGCCGATGAGCAGTGCTGCCGGGCTGGCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCC                                                                                                                                                                                                                                                     <  1:126543/235‑1   GTCAAGTTCGGGACGAATGCGCGTGAGGATCAGCATGGCAAAGGCCAGCAATAAAATGCCAGCGCCAGTAATACCCCAGCCGATGAGCAGTGCTGCCGGGCTGGCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCC                                                                                                                                                                                                                                                     >  2:126543/1‑235     CAAGTTCGGGACGAATGCGCGTGAGGATCAGCATGGCAAAGGCCAGCAATAAAATGCCAGCGCCAGTAATACCCCAGCCGATGAGCAGTGCTGCCGGGCTGGCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATT                                                                                                                                                                                                                                    >  1:26416/1‑250           CGGGACGAATGCGCGTGAGGATCAGCATGGCAAAGGCCAGCAATAAAATGCCAGCGCCAGTAATACCCCAGCCGATGCGCAGTGCTGCCGGGCTGGCAACTGCCGCCATATTTTGCGGCAGACTGAAACCACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGT                                                                                                                                                                                                                             >  1:397932/1‑251                               cacggcctgaCAAAGGCCAGAAATAAAATGCAAGCGCAAGTATCCCCCCACCCCATGAGCAGTGCTCGCGGGCTGGCATCTGCCGCCATATTTTGCGGCATACTGAAAACTCCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCATTCCCAGTTTCTTTTCCATCGATATACAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTA                                                                                                                                                                                                         <  2:102071/241‑1                                aCAGCATGGCAAAGGCCAGCAATAAAATGCCAGCGCCATTAATACCCCAGCCGATTAGCAGTGCTGCCGGGCTGGCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAG                                                                                                                                                                                                        <  1:83865/250‑1                                         CAAAGGCCAGCAATAAAATGCCAGCGCCAGTAATACCCCAGCCGATGAGCAGTGCTGCCGGGCTGTCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCATCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACG                                                                                                                                                                                               <  1:204749/251‑1                                                                         ATACCCCACCCGATGAGCAGTGCTGCCGGGCTGGCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGTAGTTTGCCACTGAAGCGCGGG                                                                                                                                                               <  2:188842/251‑1                                                                          ccCCCCAGCCGATGAGGGGGGCTGCCGGGCGGGCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGGGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATGAGGGCAAAACGCATAAAAAGGGAGTTTGCCACTGAAGCGCGGGA                                                                                                                                                              <  2:26416/249‑1                                                                                       tAGCAGAGCTGCCGGGCTGGCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGCTATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCACAACGCCTAAAAATGGTGTTTGCCACTGAAGAGAGGGATTTTACGAAG                                                                                                                                                    >  2:65678/2‑248                                                                                             TGCTGCCGGGCTGGCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCAATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGCGGGATTTTACGAAGAGATGTGTC                                                                                                                                           >  1:14641/1‑251                                                                                                  CCGGGCTGGCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGCGGGATTTTACGAAGAGATGTGTCTGCAT                                                                                                                                      >  1:193674/1‑251                                                                                                                                                     CCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGCGGGATTTTACGAAGAGATGTGTCTGCATGCAATGGTCGCGGGGAAATTTGTGACTAAAAATGCGAAAGGCGGCATCA                                                                                     >  2:320103/1‑249                                                                                                                                                                            GCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGCGGGATTTTACGAAGAGATGTGTCTGCATGCAATGGTCGCGGGGAAATTTGTGACTAAAAATGCGAAAGGCGGCATCAAGCCGCCTTATAGGAGTAACTGAAT                                                            <  1:189750/251‑1                                                                                                                                                                                          CAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGCGGGATTTTACGAAGAGATGTGTCTGCATGCAATGGTCGCGGGGAAATTTGTGACTAAAAATGCGAAAGGCGGCATCAAGCCGCCTTATAGGAGTAACTGAATAGTTATTTATACAG                                              <  1:124822/251‑1                                                                                                                                                                                               CCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGCGGGATTTTACGAAGAGATGTGTCTGCATGCAATGGTCGCGGGGAAATTTGTGACTAAAAATGCGAAAGGCGGCATCAAGCCGCCTTATAGGAGTAACTGAATAGTTATTTATACAGATCTG                                         >  1:40879/1‑251                                                                                                                                                                                               CCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGCGGGATTTTACGAAGAGATGTGTCTGCATGCAATGGTCGCGGGGAAATTTGTGACTAAAAATGCGAAAGGCGGCATCAAGCCGCCTTATAGGAGTAACTGAATAGTTATTTAGTCAGATCTG                                         <  2:235714/251‑1                                                                                                                                                                                                     TCTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGCGGGATTTTACGAAGAGATGTGTCTGCATGCAATGGTCGCGGGGAAATTTGTGACTAAAAATGCGAAAGGCGGCATCAAGCCGCCTTATAGGAGTAACTGAATAGTTATTTATACAGATCTGCGCTG                                    <  2:69288/250‑1                                                                                                                                                                                                      CTTTTCCATCGATATCCAGTGATTAAAACGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGCGGGATTTTACGAAGAGATGTGTCTGCATGCAATGGTCGCGGGGAAATTTGTGACTAAAAATGCGAAAGGCGGCATCAAGCCGCCTTATAGGAGTAACTGAATAGTTATTTATACAGATCTGCGCTGAC                                  >  2:31765/1‑251                                                                                                                                                                                                                                  CGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGCGGGA                                                                                                                                                              <  2:258169/99‑1                                                                                                                                                                                                                                  CGTGATATTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGAGCGGGA                                                                                                                                                              >  1:258169/1‑99                                                                                                                                                                                                                                      ATATTTACATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGAGGAATTTTACGAAGAGATGTGTCTGCATGCAATGGCCGCGGGGAAATTTGTGACTAAAAATGCGAAAGGCGGCATCAAGCCGCCTTATAGGAGTAACTGAATAGTTATTTATACAGATCTGCGCTGACGGTAAGGTTATTACAAAGTTCCTGCCACTGGg  >  1:143488/1‑250                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  CCGTCAAGTTCGGGACGAATGCGCGTGAGGATCAGCATGGCAAAGGCCAGCAATAAAATGCCAGCGCCAGTAATACCCCAGCCGATGAGCAGTGCTGCCGGGCTGGCAACTGCCGCCATATTTTGCGGCAGACTGAAAACACCCGCGCCCAGCATTGAGCTTAATACCAGCGCGGTGAGTGCGCTCAGTCCCAGTTTCTTTTCCATCGATATCCAGTGATTAAAACGTGATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1679762‑1679531 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTTCCATCGATATCCAGTGATTAAAACGTGATAAGAAGAATAAATATTAGGGCAAAACGCATAAAAATGGAGTTTGCCACTGAAGCGCGGGATTTTACGAAGAGATGTGTCTGCATGCAATGGTCGCGGGGAAATTTGTGACTAAAAATGCGAAAGGCGGCATCAAGCCGCCTTATAGGAGTAACTGAATAGTTATTTATACAGATCTGCGCTGACGGTCAGGTTATTACCACGTTCCTGCCACTGGC  <  NC_000913/1679563‑1679316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.