Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,302,454	Δ1 bp	coding (1273/1632 nt)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,302,454	0	A	.	100.0%	71.4 / NA	17	coding (1273/1632 nt)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (7/10);  total (7/10)

CACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGCGCACAGCTCTGTACACTAAAGCAGAACAACAGCTGGATAAGGATTCGGCCATTGTT  >  NC_000913/1302215‑1302699                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      cACCGGATACTTATGGCGCAAAATTGACACAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTTGTTAACCAGGAGTGGAAA‑CGTTCCTCGaca                                                                                                                                                                                                                                           <  2:356889/251‑1 (MQ=255)   ccGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGTAGAAAAAAATTGGTGTAAAAGTAAAACTGGTTAAACAGGAGTGGAAA‑CGTTCCTCGACAcc                                                                                                                                                                                                                                         <  1:238109/251‑1 (MQ=255)       ataCTGATGGCGCAAAATTGACGCAGCCGGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACAGTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTc                                                                                                                                                                                                                                     <  2:302817/251‑1 (MQ=255)                 cgcAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTAc                                                                                                                                                                                                                           >  1:189761/1‑251 (MQ=255)                              cAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAATCGTCAAACTGGTTAACCAGGAGTTGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGcc                                                                                                                                                                                                              <  1:370549/251‑1 (MQ=255)                                               cTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCGGGCTGGGGTGc                                                                                                                                                                                             >  2:368145/1‑251 (MQ=255)                                                 ggAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATCCCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTTGTTAACCTGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTg                                                                                                                                                                                           <  1:150093/251‑1 (MQ=255)                                                                  aaCGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAAc                                                                                                                                                                           <  1:269822/250‑1 (MQ=255)                                                                   aCGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAActt                                                                                                                                                                         <  1:156132/251‑1 (MQ=255)                                                                     tacgaagCGAAAAAACTGCTGGATGAAGCGGGTTATACCGCAGAAAAACCGTTGACAATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTATCCAGGAGTGGAAA‑CGGTACTCGACACCCGTCCACAGGGAACTTTTGATGAGGCCCGTGCAGGCTGGAGTGCTGACTACAACGAACCAActtcc                                                                                                                                                                       >  2:69834/4‑251 (MQ=255)                                                                                   aCTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACTCCTCCGATCTGCATAAAAAGCGTGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACAGGGGTGTAAACGTCAATCTGGTTAACCAGGAGTGGAAA‑CGGTCCTCGACATCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCATGCTGTTGTGCTGACTACAACTAACCAACTTCCTTCCTGAGCACCAt                                                                                                                                                         <  2:170001/251‑1 (MQ=255)                                                                                                       ataCCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGa                                                                                                                                     <  1:311502/251‑1 (MQ=255)                                                                                                                                                                                           tgtgGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAC‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGCGCACAGctc                                                 >  1:41303/1‑251 (MQ=255)                                                                                                                                                                                                        cATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAAAGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCTCAGCGCACAGCTCTGTACACTAAAGc                                    <  2:189761/251‑1 (MQ=255)                                                                                                                                                                                                                           cTGGTTAACCAGGAGTGGAAA‑CGATCCTCGACACCCGTCACCAGGGTACTTTTGATATGGCCCGTGCAGGCTGGTGTGCTGACTACAAAGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGAGCACAGCTCTGTACCATAAAGCAGAACAAAAGGTGGATa                   >  2:97759/1‑249 (MQ=255)                                                                                                                                                                                                                             ggTTAACCAGGAGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGCGCACAGCTCTGTACACTAAAGCAGAACAACAGCTGGATAAgg                >  2:3656/1‑250 (MQ=255)                                                                                                                                                                                                                                        gagtggcaaCGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGGAAGGGACTGACGGGGCGCAGCGCACCGCTCTGTACACTAAAGCAGAACAAAAGCTGGATAAGGATTGGGCCAtt     >  2:155648/8‑251 (MQ=255)                                                                                                                                                                                                                                        aGTGGAAA‑CGTTCCTCGACACCCGTCACCAGGGGACTTTTGATGAGGCACGTGCAGGCGGCTGGGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGATTTCGAACAGCTCGTACAATACAGCGCCTTATAAGAGCCCGGCCTTTGACAGCATTACGGCGGAAACACTTAAAATGACTGACACGGCGAAGCGCACAGCTCTGGAAAATAAAGACGAACCACGGCTGGATacgacttgggcaattt    >  2:39482/1‑236 (MQ=255)                                                                                                                                                                                                                                            ggaaaCGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGCGCACAGCTCTGTACACTAAAGCAGAACAACAGCTGGATAAGGATTCGGCCATTGtt  <  1:298096/248‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      CACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGCGCACAGCTCTGTACACTAAAGCAGAACAACAGCTGGATAAGGATTCGGCCATTGTT  >  NC_000913/1302215‑1302699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑