Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,111,259	Δ1 bp	coding (718/900 nt)	rfbD ←	dTDP‑4‑dehydrorhamnose reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,111,259	0	G	.	100.0%	130.5 / NA	29	coding (718/900 nt)	rfbD	dTDP‑4‑dehydrorhamnose reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (19/10);  total (19/10)

TCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTA  >  NC_000913/2111028‑2111497                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               tCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCct                                                                                                                                                                                                                            >  1:211261/1‑251 (MQ=255)   cTTTTAATTCATCTTGCTCAACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCctct                                                                                                                                                                                                                          >  1:107366/1‑251 (MQ=255)          ttCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGCGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCaaaa                                                                                                                                                                                                                    >  1:64966/1‑250 (MQ=255)               cgtGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGGTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAAGTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAg                                                                                                                                                                                                               <  1:146179/248‑1 (MQ=255)                 tGCTCCACCATCACTATCAAGATGCAAAAACTATTAAAGTGCTGTAGTCGTAAATAATTCATTGAGCATTCGGTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGTCGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAgcg                                                                                                                                                                                                             <  2:141595/250‑1 (MQ=255)                                               cTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACt                                                                                                                                                                              >  1:61540/1‑251 (MQ=255)                                                         gCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCaa                                                                                                                                                                     >  1:81400/1‑250 (MQ=255)                                                            gTAGTCGTAAATAATTCATTGAGCATTCGTTTAACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATgg                                                                                                                                                                 >  1:290490/1‑251 (MQ=255)                                                                  gTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGAAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACa                                                                                                                                                             >  2:287/1‑249 (MQ=255)                                                                   tAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTCCCACTGGCTACCAAATGGAACAAGc                                                                                                                                                          >  1:328972/1‑251 (MQ=255)                                                                    aaaTAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTGCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTTCAAGcc                                                                                                                                                         <  2:290490/251‑1 (MQ=255)                                                                                gAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCGTCAAAAACCAGCGCAGCATAATCGTTCCAGGTGGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCg                                                                                                                                              <  1:346515/250‑1 (MQ=255)                                                                                       cGTTTCTCGCCAAACTGCCAGACAGGCAAGAAACGCGCCAAGTTCTCCTGAAATTTTTCTGTATTACGGCAAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCACAAACCAGCGCCGCATAATCGTACCAGGTTGTGGTACCACTGGATACCAAATGGTACACGCCTTCGACATCCGGTTTAtt                                                                                                                                       >  2:370525/1‑250 (MQ=255)                                                                                              cGCCACCCGGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGt                                                                                                                                   <  2:276107/247‑1 (MQ=255)                                                                                              cGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGt                                                                                                                                   >  1:276107/1‑247 (MQ=255)                                                                                                        ccAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGc                                                                                                                     >  1:216566/1‑251 (MQ=255)                                                                                                          aGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCAt                                                                                                                   >  1:144911/1‑251 (MQ=255)                                                                                                               ggCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCt                                                                                                              <  2:81400/251‑1 (MQ=255)                                                                                                                    gACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTAc                                                                                                          >  2:94621/1‑250 (MQ=255)                                                                                                                                                        aaGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTCCTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATc                                                                                                      >  2:23638/1‑218 (MQ=255)                                                                                                                                                        aaGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATc                                                                                                      <  1:23638/218‑1 (MQ=255)                                                                                                                                                              agagTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCaa                                                                                                                                                                     <  1:391688/149‑1 (MQ=255)                                                                                                                                                              agagTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATc                                                               >  1:269855/1‑251 (MQ=255)                                                                                                                                                              agagTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGATTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCaa                                                                                                                                                                     >  2:391688/1‑149 (MQ=255)                                                                                                                                                                             acgaGCTGGTGTAGGATAGGATGTTGTTGGTACTGCGTTGAGCTTGTTGGGGGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGGTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATACGGATTATTCAGTGCGACACGAATGGCATGTGCTGTACAAGCAGCCAGCAGTTCAGCACCTGTTGGAGCACCAAACTGATCGTTAATACCCGCTa                                                 >  2:35317/1‑250 (MQ=255)                                                                                                                                                                               gaGCTGTTGTAGGATAGGCTGTTTTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTCCCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAAtt                                              <  1:198089/251‑1 (MQ=255)                                                                                                                                                                                                               tcGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGtt              >  2:113389/2‑251 (MQ=255)                                                                                                                                                                                                                  ttGAGCTTGTTGCGTGCAAGGG‑CGCGCCTGCTTTGCCCGCCTGTTCAAAACCCAGGGCAGCAAGATCGTACACGGTTGTGCGAAAAGTGGCTACCAAATGGTACAAGACTGCGACCTCCGGTTTATTAAGTGCGACCCGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCCCCTGTTGGCGCACCATACTGATCGTTAATAACCGCTAATTGTTCACCCTCTTTTGCCAGACTTAACATCTTTTTg           >  2:156960/1‑251 (MQ=255)                                                                                                                                                                                                                            tgAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTa  <  1:94621/250‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               TCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTA  >  NC_000913/2111028‑2111497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑