Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	480,295	2 bp→AA	coding (13‑14/219 nt)	hha ←	hemolysin expression‑modulating protein Hha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	480,295	0	G	A	100.0%	45.1 / NA	17	P5F (CCT→TTT)	hha	hemolysin expression‑modulating protein Hha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/6);  total (11/6)
*	NC_000913	480,296	0	G	A	100.0%	52.5 / NA	17	P5F (CCT→TTT)	hha	hemolysin expression‑modulating protein Hha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/6);  total (11/6)

ATAAACAGCCGGTTATAGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATT  >  NC_000913/480052‑480528                                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                         ataaACACCCGGTTATAGCTCCGAAAGCGGCTTATTTAATAGCGAATAAATTTCCATACTGCCGACGGGATCTTGTCGTACAGCTTATTCATGGTCAATTCGGCGCGGCGGTGATCTGCGGCTGAGTAAAATACCGCCCGTTCCTTATCCGATAATTCGTATTTATTTTTCTCGCTAACACGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAACCGCATTAAATAATCGGTTTTCGTTAAAAATTTTTc                                                                                                                                                                                                                                    >  2:63458/1‑251 (MQ=255)                                            gAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGT‑CAATTGTCTGGCCACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATAAGTCTACCTATGGTTGTAATAATTCTAACAAGATa                                                                                                                                                                                          >  1:202331/1‑250 (MQ=255)                                                         tACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGCTCTGCGGCTGAGTAAAATAACGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAAATCGCAGGAttt                                                                                                                                                                            >  2:450730/1‑250 (MQ=255)                                                                                   gTTTATTAATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACg                                                                                                                                                  <  1:124833/251‑1 (MQ=255)                                                                                                              gttgatcggaggCTGTGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGGTGTAATAATTCTAACAAGATAAACTCGCAGGATTCGCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCa                                                                                                                       <  2:58362/241‑1 (MQ=255)                                                                                                                          cTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGa                                                                                                                >  1:264904/1‑246 (MQ=255)                                                                                                                              gTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTGTCGTCGCATTGACAAAACAACTGAATAGTCGATTACCTGAGTTCCGCCATTGCTGAAGATCCTGc                                                                                                       <  2:272073/251‑1 (MQ=255)                                                                                                                                                aTTATCTGCTAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAact                                                                                      >  2:121332/1‑250 (MQ=255)                                                                                                                                                       gATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                                                                                <  1:289596/249‑1 (MQ=255)                                                                                                                                                               gTATTTATTTTTCTCGATAACACGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTAGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATCACTACTGACCAACATaa                                                                        >  2:230107/1‑249 (MQ=255)                                                                                                                                                                       ttttCTCGATAACACGCTCCAGC‑TGTTCAATTGTCTGTCAACTACGTGAACGTATTAAAGAATCGTTTTGCGTTAAAAATTTTTCGGAGATACTTCTACCTATGGTTGTAATAATTCTAACAATATAAACTCGCAGTATTCTCCTTCGTGGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATGTCTGAAGTTTCTGCATATTAAAACTATCACTTCTGAACCACAtttaggtatcat                                                               <  2:211941/250‑12 (MQ=255)                                                                                                                                                                              gATAACACGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGatat                                                       >  1:200208/1‑251 (MQ=255)                                                                                                                                                                                    acGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAAAAGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTTGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATtcgtcg                                                 >  1:4608/1‑251 (MQ=255)                                                                                                                                                                                                                  gacgTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATACTTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCAtt                    >  1:200951/1‑251 (MQ=255)                                                                                                                                                                                                                  gacgTAAACGCATTAAATAATCGGTTTTCGTTAAAAAGTTTTCGGACATACTTCGACCTATGGTTGTAATAATTCTAACAAGATAAACGCGCAGGATTCTCTGTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCAtt                    <  1:207354/251‑1 (MQ=255)                                                                                                                                                                                                                         aCGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCCTTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACtt              >  2:353554/1‑250 (MQ=255)                                                                                                                                                                                                                                    aatCGGTTTTCGTTAAAAATTTTTCGGACCTACTTATACCTATGGCTGTAATAATTATAACAAGATAAACTAGCAGGCTTCTCTTTAGTCGCATTGACAAAACAACGGCAGAGTCGATAACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATACAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGATTATTGTCTTAATTCTACTTAATTTTGTAAtt  >  2:409756/1‑251 (MQ=255)                                                                                                                                                                                                                                                     ||                                                                                                                                                                                                                                         ATAAACAGCCGGTTATAGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGT‑CAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATT  >  NC_000913/480052‑480528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑