Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,028,546	G→A	A156V (GCC→GTC)	ygfS ←	putative electron transport protein YgfS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,028,546	0	G	A	100.0%	81.0 / NA	25	A156V (GCC→GTC)	ygfS	putative electron transport protein YgfS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/10);  total (15/10)

CGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAATTTGTTGTCGGGTATCCCCCGGCAACGACTGAATGGTGATCATTCCAAACGGGCACGCACTGACGCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCT  >  NC_000913/3028332‑3028786                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 cgtgcTGACTTGTAATAACGGTACCGGGTTTATGCTGGGCTTTGTGACACTGGTCGGAGGCCGCGTTTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGACTTCTTTCAGAGAGGATGCTCACCGCTGACAACAATACGCTGCTTGCGGTCTTGCCTGAGTtct                                                                                                                                                                                                               <  2:58987/246‑1 (MQ=255)             tAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGc                                                                                                                                                                                                   >  1:181240/1‑250 (MQ=255)                tatCGTTACCGGTATTATGCTGAGCTTTGAGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGCGTTCTCTTTCAGTAAGCAAcc                                                                                                                                                                                               >  2:168944/1‑250 (MQ=255)                       aCCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAgcgc                                                                                                                                                                                        <  1:265428/251‑1 (MQ=255)                        ccGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACgctg                                                                                                                                                                                                                                     <  2:81345/205‑1 (MQ=255)                        ccGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACgctg                                                                                                                                                                                                                                     >  1:81345/1‑205 (MQ=255)                                  gCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGc                                                                                                                                                                             <  2:360403/251‑1 (MQ=255)                                          ttGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCCAGATTc                                                                                                                                                                      >  2:267102/1‑250 (MQ=255)                                                cacTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGc                                                                                                                                                                 >  1:406824/1‑249 (MQ=255)                                                       cGTAGGCCGCGTCTTTGCCCGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCCCCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCTCCTGTGTTGGGCAAGAGTCAACGCAGGTTGGcc                                                                                                                                                        <  1:100459/251‑1 (MQ=255)                                                               gcgTCTTTGCACGCGAATGGCAAAAGCTGAAGATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTcc                                                                                                                                                <  2:105524/251‑1 (MQ=255)                                                                        cACGCGAATGGCAAACGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGGGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTc                                                                                                                                        >  2:51934/1‑250 (MQ=255)                                                                           gcgAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTcac                                                                                                                                      >  1:88372/1‑249 (MQ=255)                                                                                             atatTGGTACGGGGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAAtt                                                                                                                  <  2:118904/251‑1 (MQ=255)                                                                                                  ggTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAATttgttt                                                                                                             >  1:245237/1‑250 (MQ=255)                                                                                                       ggTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGTGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAATTTGTTGTCggg                                                                                                        >  2:248344/1‑251 (MQ=255)                                                                                                                  cTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTATTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAATTTGTTGTCGGGTATCCCCCGGc                                                                                             >  1:60333/1‑251 (MQ=255)                                                                                                                     ccGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGGGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAATTTGTTGTCGGGTATCCCCCGGCAAc                                                                                          <  1:51934/251‑1 (MQ=255)                                                                                                                                 cattttcagcgGGTGGTTGGGCACTCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACACCAATACGCTGCTGGCGGACTCGCCTGAATTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAATTTGTTGTCGGGTATCCCCCGGCAACGACTGAATGGTg                                                                              >  2:424552/9‑251 (MQ=255)                                                                                                                                        cgcgGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAATTTGTTGTCGGGTATCCCCCGTCATCTACTGAATGGTGATCATTc                                                                       <  1:316288/251‑1 (MQ=255)                                                                                                                                                                     tcCGCGCCTTTAAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAATTTGTTGTCGGGTATCCCCCGGCAACGACTGAATGGTGATCATTCCAAACGGGCACGCACTGACGCAACTCTg                                           >  2:235657/1‑250 (MQ=255)                                                                                                                                                                                    gcgGAGATTTCTTTAAGAGAGGATTCTCACCGCTGACAACACTACGCTGCTGGCGGACTCGACTGAGTTCTATTTCAGTAAGCAACTGCAGCGCATGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTATTCCCGCTGTTCACAGAGATCGCATTTCACAATTTGTTGTCGGGTATCCCCCGGCCACGACTGAATGGTGATCATTCAAAACGGGAACGAACTGAAGCAACTCTGACAGCCAATACAACgg                           >  2:427988/1‑251 (MQ=255)                                                                                                                                                                                                ttcAGAGAGGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAATTTGTTGTCGGGTATCCCCCGGCAACGACTGAATGGTGATCATTCCAAACGGGCACGCACTGACGCAACTCTGACAGCCAATACAACGGGCAGCATTGGTc               >  2:147258/1‑251 (MQ=255)                                                                                                                                                                                                       agGATTCTCACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTTGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAATTTGTTGTCGGGTATCCCCCGGCAACGACTGAATGGTGATCATTCCAAACGGGCACGCACTGACGCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGcacca        <  1:184932/251‑1 (MQ=255)                                                                                                                                                                                                             ctcACCGCTGACAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGCGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAATTTGTTGTCGGGTATCCCCCGGCAACGACTGAATGGTGATCATTCCAAACGGGCACGCACTGACGCCACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGcc  >  2:238752/1‑250 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 CGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATTCAACGCAGGCTGGCCCCTCTTCCCGCTGTTCACAGAGATCGCATTTCACAATTTGTTGTCGGGTATCCCCCGGCAACGACTGAATGGTGATCATTCCAAACGGGCACGCACTGACGCAACTCTGACAGCCAATACAACGGGCAGAATTGGTCTGCACCACCTGCT  >  NC_000913/3028332‑3028786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑