Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,867,454	T→A	Q33L (CAG→CTG)	rpoS ←	RNA polymerase sigma factor RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,867,454	0	T	A	100.0%	152.9 / NA	46	Q33L (CAG→CTG)	rpoS	RNA polymerase sigma factor RpoS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (19/27);  total (19/27)

GCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGT  >  NC_000913/2867222‑2867700                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       gCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCttt                                                                                                                                                                                                                                       <  1:276650/250‑1 (MQ=255)            gggCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAAcc                                                                                                                                                                                                                           >  2:173922/1‑251 (MQ=255)             ggCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTGTCGTCAAAAACCt                                                                                                                                                                                                                          <  1:123521/251‑1 (MQ=255)                aaTTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCACATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCa                                                                                                                                                                                                                        >  2:178586/1‑250 (MQ=255)                     ttACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg                                                                                                                                                                                                                  >  1:370255/1‑251 (MQ=255)                      tACCCCCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAATCCTCATCTCCg                                                                                                                                                                                                                  <  2:393114/250‑1 (MQ=255)                       accaccAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGtt                                                                                                                                                                                                                <  1:397779/251‑1 (MQ=255)                         caccaGACGCAAGTTTCTCTCCATCATCCGTCGGCGATAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATCAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGGCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCTCTGGGGTCCAGTTCTATTAAGTCTTTTTCGTCaaaaatatcatcgccgttct                                                                                                                                                                                                              <  2:297947/251‑16 (MQ=255)                                  cAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTTAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTGCGTCAAAAACCTCAACTCCGTTCTCATCAAAtt                                                                                                                                                                                                     <  2:151795/251‑1 (MQ=255)                                     gTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCg                                                                                                                                                                                                  <  1:242652/251‑1 (MQ=255)                                        aCTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCGTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGTCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCAt                                                                                                                                                                                               <  1:425705/251‑1 (MQ=255)                                                ccatcCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTTGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCAttt                                                                                                                                                                                       <  2:191614/250‑1 (MQ=255)                                                    ccGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAAt                                                                                                                                                                                   <  2:14495/251‑1 (MQ=255)                                                        cggcgAGAGGCGACATCTACACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGCAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTCACTCATg                                                                                                                                                                               >  2:303341/1‑251 (MQ=255)                                                           cgAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAAc                                                                                                                                                                            <  2:2799/251‑1 (MQ=255)                                                               agGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGGCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACGCCGTGCTCAGCAAATTCCGCATCTTCATTTAAATCATGATCTTTc                                                                                                                                                                        <  2:323647/251‑1 (MQ=255)                                                                ggCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTcc                                                                                                                                                                       >  2:272185/1‑250 (MQ=255)                                                                ggCGACATCTCAACGGAGTGAGCGCCGCGCAAAATACACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTCTCACTGGGTTCAAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCAGTTCTCATCAAATTCCGCATCATCATTTAAATCATGAACTTTc                                                                                                                                                                        >  2:37520/1‑250 (MQ=255)                                                                     cATCTCCTCGCATTGCGCGACGCGCAAAATAAACTTCTTCTACGGCCGTAAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCGGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTTCGATAACATTTCCTCTTCGGCCAATTCGTTATCACTGGGTTCCAGTTCTACTAAGTCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTTCGCATCTTCATTTAAATCATGCACtcgcagcgga                                                                                                                                                                  <  2:281287/251‑10 (MQ=255)                                                                          ccACTCAGTGCGCGACGCGCAAAATAAACGTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTTGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACGTCAACGCCGTTCTCAGCAAATTCCTCATCTTCATTTAAATCATGAACTTTCAGCGTAt                                                                                                                                                                 <  2:207157/247‑1 (MQ=255)                                                                                aGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCAt                                                                                                                                                       >  1:104643/1‑251 (MQ=255)                                                                                  tGCGCGACGCGCAACATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATa                                                                                                                                                      >  1:70327/1‑250 (MQ=255)                                                                                   gcgcGACTCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTAGCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTGCTCATCAAATTCCGCATCTTCAGTTAAATCATGAACTTTCAGCGTATTCGGACTCagaa                                                                                                                                                     <  2:421809/250‑4 (MQ=255)                                                                                            gcAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCt                                                                                                                                           <  2:118417/251‑1 (MQ=255)                                                                                             cAAAATAAACTTCTTCTGCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTa                                                                                                                                          <  1:228781/251‑1 (MQ=255)                                                                                                                 gcctttAACAGTGATGAATAGCCAATCTCACCACGGTAAAGCTTATTCTCTTCCAACACACGCTGATTGGCTACCTTCGATAACAGTTCCTCTTCGGCCACAACTTTATCTCTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTTCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATTTACTTTCAGCGTATACTGACTCATAAGGTGGCTCCTACCCGTCATCCCTTGCCGGaa                                                                                                                      <  2:91197/247‑1 (MQ=255)                                                                                                                   cGTTAACAGTGGTGAATAACCACTCTCACCAAGGTAAAGCTGCGTCGCGTCCAACACACGATGTGAGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAACTCGTTATCACTGGGTTCCAGTTCTACTAAGCCCTTTTCGTCAAAAACCTCAACTCTGTTCTCATCAAATTCCGCATCTTCATTTAAATCCTGAACTTTCAGCGTATTCTGCATCATAAGGTGGCGACTACTCGTGATCCCTTGCCggcac                                                                                                                     >  2:410628/1‑247 (MQ=255)                                                                                                                     ttagaagcggtgAATAACCAATCTCACCAAGGTAAAGCTGAGGCGAGTCCAACACAAGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAAGCGTTATCACTGGGTTCCAGTACTACTAAGGCCTTTTCGTCAAAAACCTCAAATCCGTTCTCCTCAAATTACGCATCTTCATTTAAATCATGAACTTTCAGCGTATTATGACTCATAAGGTGGCACCTACCCGTGATCACTTGACGGAACAtt                                                                                                                  >  2:315294/9‑251 (MQ=255)                                                                                                                     ttAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACAtt                                                                                                                  >  1:365376/1‑251 (MQ=255)                                                                                                                                  aTAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCt                                                                                                     <  1:48206/251‑1 (MQ=255)                                                                                                                                          tctcACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGCTAACAGTTCCTCTTCGGCCAAATCGATATCACTAGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATATTCATTTAAATCATGAACTTTCAGCGTATTCTGACTAATAAGGTGGCTCCTACCCGTGATCCCTTGCCGGAACATTCAAGCAAAAGACTGGTTccgc                                                                                             >  1:56458/1‑251 (MQ=255)                                                                                                                                           ctcACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTccgcc                                                                                            >  2:77202/1‑251 (MQ=255)                                                                                                                                                    gTAAAGCTGATTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTGCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCg                                                                                   <  2:128086/251‑1 (MQ=255)                                                                                                                                                     tAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTACGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGc                                                                                  >  2:381126/1‑251 (MQ=255)                                                                                                                                                         gCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCg                                                                              <  1:326196/251‑1 (MQ=255)                                                                                                                                                                cgcgTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTTCCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAAt                                                                        <  1:173922/250‑1 (MQ=255)                                                                                                                                                                              gctttgtgtcgcccTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTa                                                         <  2:136067/240‑1 (MQ=255)                                                                                                                                                                                tgtgtgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACg                                                       >  2:200850/1‑251 (MQ=255)                                                                                                                                                                                       cTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTcc                                                >  1:9402/1‑251 (MQ=255)                                                                                                                                                                                          ccTGCGATAACATTTCCTCATCGGCCAAATCGTTATCACTGGGTCCCAGTTCTTCTAAGACATTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTCCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGACCCCTTGACGTAACATTCAAGCAACAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGAGGTTTTACGGATTTCCCCt                                             <  2:368638/251‑1 (MQ=255)                                                                                                                                                                                          ccTGCGATAACAGTTCCTCTGCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCt                                             <  2:167800/251‑1 (MQ=255)                                                                                                                                                                                          ccTGCGATAACAGTGACTCGTCGGCCAAATCGTTATCACTGGGTTCCAGGTCAACGAAGGTCTTTTCGTCAAAAACCTCAACGCAGTTCTCATAAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTACTACCCGTGATCCCTGGACGGAATATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAAGAACGCAGCGGGTTTACGGATTTCCCCt                                             <  2:167818/251‑1 (MQ=255)                                                                                                                                                                                             gCGATAGCAGTTCCTCGTCGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGt                                          <  1:56901/251‑1 (MQ=255)                                                                                                                                                                                                               cGGCCAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGc                         >  1:362786/1‑250 (MQ=255)                                                                                                                                                                                                                   cAAATCGTTATCACTGGGTTCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTAATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGCGATCCATTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCg                    >  2:458555/1‑251 (MQ=255)                                                                                                                                                                                                                                     ttCCAGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGGTTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCggg  >  1:420262/1‑250 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       GCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCGGT  >  NC_000913/2867222‑2867700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑