Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,732,992	G→A	I394I (ATC→ATT)  I394I (ATC→ATT)	clpB ← clpB ←	chaperone protein ClpB chaperone protein ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,732,992	0	G	A	100.0%	161.6 / NA	45	I394I (ATC→ATT)  I394I (ATC→ATT)	clpB clpB	chaperone protein ClpB chaperone protein ClpB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/16);  total (29/16)

GCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCGATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTC  >  NC_000913/2732757‑2733232                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 gCGTACCAGAACGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCGTCATCAATCAGGTCGATg                                                                                                                                                                                                                                      <  1:80229/248‑1 (MQ=255)   gTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTTTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCttt                                                                                                                                                                                                                                 >  1:69690/1‑251 (MQ=255)       cAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATcc                                                                                                                                                                                                                             >  1:51943/1‑251 (MQ=255)              cGATGCCTTCTCTGCTTTACACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGCCTCTTTCCTTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCt                                                                                                                                                                                                                      >  2:399119/1‑251 (MQ=255)                  gCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTATGGTTTTGAGTCACTCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACg                                                                                                                                                                                                                  >  2:439903/1‑251 (MQ=255)                   ccTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACgg                                                                                                                                                                                                                 <  1:137623/251‑1 (MQ=255)                                ccACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGc                                                                                                                                                                                                    <  1:225724/251‑1 (MQ=255)                                 cACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCg                                                                                                                                                                                                   >  2:53613/1‑251 (MQ=255)                                       tcttctAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATg                                                                                                                                                                                                >  1:277105/1‑248 (MQ=255)                                             aaCTCGGAGTACTGACGTTATTTGTCGATCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTACAGTTTGAGCTGGCTGATACGACGAACGAGTCGGTCGAGATCTTCTGGGTTTGAGTCAATCTGCATACGAATGCGGGATGCTGCTTCATCAATCCGGTCGATGGCTTTATCAGGCAGTTGACGGTCAGCAATTTAGCGGTGAGACacagt                                                                                                                                                                                       >  2:399345/1‑247 (MQ=255)                                                      tACTGACGATCTTTGTCGCTCAGTTCTTTGTTGAGCATATACAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCCGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGCGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTTTCCGGCAGCTGCCGGTAATCAATGTAGCGATGAGACAACGTCGCCGCTGc                                                                                                                                                                              >  2:383925/1‑251 (MQ=255)                                                          taCGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGGCGATGGCTTTATCCGGCCGCTGACGGTGAGCAATGTAGCGATGAGACAACGTcgccgacgcaac                                                                                                                                                                           <  2:330607/249‑8 (MQ=255)                                                           aCGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGcaaca                                                                                                                                                                          >  1:24323/1‑250 (MQ=255)                                                                  ttGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTGCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTTACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAATAATTGCCg                                                                                                                                                                   <  2:444152/250‑1 (MQ=255)                                                                    gTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCgggg                                                                                                                                                                >  1:352914/1‑250 (MQ=255)                                                                                ttcGTTGAGCATATCACGACGTTTTTTGCTGGCTTCATCAGACTCTTTCATCAACGCCTGTCGTTCCAGTTTGCGCTGGCTGATCCGACGATTGAGTCGGTCGAGTTTTTCTGGGTTTGAGTCAATCTGCATTCGAATGCTGGATGCTGCTTCCTCAATCCGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGCCAACGTCGCCGCTGCACCAATTGCCGGATCCATAATTTGc                                                                                                                                                     >  2:461566/1‑250 (MQ=255)                                                                                   gTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACTATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGGTGAGACAACGTCGCCGCTGCAACAATTGCCGGGCTAGAAATTTGCACAt                                                                                                                                                 >  2:259133/1‑251 (MQ=255)                                                                                                aGACGTTTTTTACTGGCTTCATCAGACTCTTTAATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGATGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTACTTTGCACATGGTGGTGCAATTc                                                                                                                                    >  1:380209/1‑251 (MQ=255)                                                                                                      tttttCCTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGCTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCCGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCCGTAATTTGCACATGGTGGTGCAATTCgacacg                                                                                                                              >  2:67811/1‑246 (MQ=255)                                                                                                          tACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGGGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGAt                                                                                                                                                                                                                                       >  2:77664/1‑142 (MQ=255)                                                                                                          tACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGAt                                                                                                                                                                                                                                       <  1:77664/142‑1 (MQ=255)                                                                                                          tACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTc                                                                                                                           <  1:315380/250‑1 (MQ=255)                                                                                                           aCTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCtt                                                                                                                         >  2:233937/1‑251 (MQ=255)                                                                                                           aCTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCt                                                                                                                          <  1:53613/250‑1 (MQ=255)                                                                                                                  tcatcaGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAgg                                                                                                                    <  2:98967/249‑1 (MQ=255)                                                                                                                    atcaGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCAc                                                                                                                >  1:213356/1‑251 (MQ=255)                                                                                                                            ctTTCATTAACGCCTGTTGTTCCAGATTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGAGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATc                                                                                                        >  2:298060/1‑251 (MQ=255)                                                                                                                                 aTTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCCCGCAGAATCGCAAt                                                                                                   >  1:229718/1‑251 (MQ=255)                                                                                                                                    aaCGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGt                                                                                                >  1:113202/1‑251 (MQ=255)                                                                                                                                          tgttgtTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTc                                                                                          >  1:361552/1‑251 (MQ=255)                                                                                                                                          tgttgtTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTc                                                                                          >  1:254952/1‑251 (MQ=255)                                                                                                                                                tCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAg                                                                                     <  2:254952/250‑1 (MQ=255)                                                                                                                                                             ggATGATACGACGATCGAGTCGGTCGAGTGCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGcaac                                                                        <  1:439903/250‑1 (MQ=255)                                                                                                                                                                            cGAGTCGGTCGAGTTATTATGGTTTTGAGTAAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGaa                                                         >  1:395547/1‑250 (MQ=255)                                                                                                                                                                                        gTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTGTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAgc                                            <  1:280043/251‑1 (MQ=255)                                                                                                                                                                                         ttcttcTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAgcg                                           >  1:184593/1‑251 (MQ=255)                                                                                                                                                                                          tcttctGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGATTCATCAATCAGGTCGATTGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGGGGTTCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACTACTTTCCAgcg                                           <  2:181348/250‑1 (MQ=255)                                                                                                                                                                                                    ttGAGTCAATCTGCATTCGGATGCTGGATGCTGCTTCAGCAATCAGGTCGATGGCTTGATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTGCTGGAAACGACGTTCCAGCGCAGCATCttt                                 <  1:114824/250‑1 (MQ=255)                                                                                                                                                                                                     tGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCAGGAAACGACGTTCCAGCGCAGCATCttttt                               >  1:320157/1‑251 (MQ=255)                                                                                                                                                                                                      gAGTCAATCTGCATACGAATTCTGGCTGCTGCTTCATCACTCAGGTCGATGGCTTTATCCGACAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCCACAATTGACGGGTAAGTAATTAGCACATGGTGGTGCAATTCGTCACGTTCTTTCAGGCCACGAGGAATCGCACTGGTATCTTCAACAGCAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCttttt                               >  2:157703/1‑250 (MQ=255)                                                                                                                                                                                                          cAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGGCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAAt                           <  2:277105/250‑1 (MQ=255)                                                                                                                                                                                                               tGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGCGACAACGTCGCCGCTGCAACACTTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTg                     >  2:297696/1‑251 (MQ=255)                                                                                                                                                                                                                             ggATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGGGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCa        >  2:20319/1‑250 (MQ=255)                                                                                                                                                                                                                              gATGCAGCTTCATCAATCAGGTCGCTGGCTTTATCCGGCAGCTAACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCCATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCa        >  2:337938/1‑249 (MQ=255)                                                                                                                                                                                                                                    gctTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGcgtc  <  1:297696/249‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 GCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCGATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTC  >  NC_000913/2732757‑2733232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑