Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,510,609 C→T P327L (CCG→CTG)  yfeO → putative transport protein YfeO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,510,6090CT96.8% 89.1 / ‑3.5 32P327L (CCG→CTG) yfeOputative transport protein YfeO
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (23/7);  total (24/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01

CCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAG‑TCG‑TTGT‑ACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGG  >  NC_000913/2510362‑2510845
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               
ccgcggtggccgtcgATTATGCATCAAATGAAAAATCCGGTGCTCGTGGTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCCTCAGCGATTACTTTTTGCTGGCGGTAATTAATCTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGTTAATCTTCCTGGc                                                                                                                                                                                                                                              <  2:11777/238‑1 (MQ=255)
     gTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCCGTTTCGCTGTTTAAAGGACTGGATGAGATGCAGCAAATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTTAAATTTCCCCCCTGGTCGTTTCTTCCGCCCGTGGCTTTTGCGGTGTGCCCATCTTCCTGGCCgtgt                                                                                                                                                                                                                                         >  1:409737/1‑251 (MQ=255)
              gatgatGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGATATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTTGTCGGTGCTGCCGCCAGTGGCTTTTGCGGGGGGCGGATCTTTCTGGCAGGGTTTGTCGGcc                                                                                                                                                                                                                                >  1:185351/1‑250 (MQ=255)
                  atgCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTAGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGc                                                                                                                                                                                                                            <  1:436730/251‑1 (MQ=255)
                   ggCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCGGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCa                                                                                                                                                                                                                           <  2:358631/250‑1 (MQ=255)
                            tGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTgg                                                                                                                                                                                                                             <  1:49821/240‑1 (MQ=255)
                              aaaaaTCCGGTGCTCGGGCTGGGTGTTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGAGGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGTCCTGGTCGTTGCTGCCGCCAGTGGCTTGCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGAt                                                                                                                                                                                                                <  2:222094/251‑1 (MQ=255)
                                aaaTCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCGGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGAtgc                                                                                                                                                                                                              <  2:116557/251‑1 (MQ=255)
                                       gTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGa                                                                                                                                                                                                       <  1:170268/251‑1 (MQ=255)
                                            cGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCCGCTGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTTAACTTGCCGCCCCGGGCGTTACTGCCGCCAGTGGCTTTTGCGGGGGGCGAATCTTCCTGGGAGATTTTGTTGGCGGGGGATTAGGGGTGagtctggcagctcccg                                                                                                                                                                                                  >  1:280786/1‑236 (MQ=255)
                                                cTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGGCGTTTCTGCCGCCAGTGGCTTTCGCGGGGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGGATTAGGGGTTATGCTGCATGAGCACGtttc                                                                                                                                                                                              >  1:444225/1‑249 (MQ=255)
                                                    ggaTTGGCGGATTTAGTCTCGGTATTCGGGGGGTGTTTGGTGGACCAGTTTCGCGGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAATTCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCTCGGTGGGCGAATCTTCCTGGCAGGGTTGGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCccgcc                                                                                                                                                                                          <  1:412088/249‑1 (MQ=255)
                                                                    tctcGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGGGCACGTTCCCGCCGTACCAGCGGCAATa                                                                                                                                                                           >  1:349521/1‑250 (MQ=255)
                                                                               tGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCCGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTgc                                                                                                                                                               >  1:154569/1‑251 (MQ=255)
                                                                                        ttGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGGTGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCCGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATACTGCATGAGCACGTTCCCGCCGGACCAGCGGCAATAACCGTTTCTTGCGCTATtctc                                                                                                                                                      >  1:392200/1‑251 (MQ=255)
                                                                                                       cGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGACAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAGTTAAACTCGCCGCCCTCGTCGTTGCTTCCGCCAGTGGCTTTCGCCGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGTTGCTTCAAGAGGACGTTCCCGCCGTACCAGCGGCAATAACCGGTTCTTggtctattttacgcacactgctgggg                                                                                                                                       >  2:455822/1‑226 (MQ=255)
                                                                                                          tGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGGGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTggtggt                                                                                                                                     >  1:198955/1‑250 (MQ=255)
                                                                                                             ttAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCCCCCTGGCCGTTGCTGCCCCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCCTCGTGCTGGTGGTAac                                                                                                                                  >  1:157390/1‑250 (MQ=255)
                                                                                                                      tGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGc                                                                                                                        >  1:87184/1‑251 (MQ=255)
                                                                                                                             gaTGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGGGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGGTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTa                                                                                                                  >  1:391524/1‑250 (MQ=255)
                                                                                                                                  agcagATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGGCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCtt                                                                                                            >  1:32225/1‑251 (MQ=255)
                                                                                                                                  agcagATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGGCGTTGCTGCCGCCAGTGGCTTTCGCGGGGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGAGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCtt                                                                                                            >  1:242199/1‑251 (MQ=255)
                                                                                                                                  agcagATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGACGTTGCTGCCGCACGTGGCTTTCGCGGAGGGAGAATATTCCTGGCAGTGTTTGTCGGCGGGGCATTAGGGTTGATGATGCCTGAGCACGGTCCCGCCGGACCAGCGGCAATAACCGTTTTTTGCGCTTTTCTCGGGATCGTGCTGGTGGGAACACGCGATGGCTGGTTAAGtatt                                                                                                            >  1:232115/1‑248 (MQ=255)
                                                                                                                                              cAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCCGTGTTTGTCGGCGTGGCATTAGGGTTGATGGTGCATGAGCACGTTCCCGCCGTACCAGCGGCCATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATtgcggcc                                                                                                >  1:25368/1‑250 (MQ=255)
                                                                                                                                               aaaTCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTCAAATTGCCGCCCTGGGCGGTGCTCCCCACACCCGCCTTCCCGGAGGGCCAAACTTCCTGGCACTGTTTGTCCGCGTGGCATTAGGGGTGATGCTGCATGAGCAAGGTCCCGCCGTACCATCGGCAATACCCGGTTCTTGCGCTATTCTCGGCATCCGGCTGGTGGGAACACGCGATAGATGGTTTAGTCTTTTTTTggcggtac                                                                                               >  1:412056/1‑248 (MQ=255)
                                                                                                                                                    aGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTTAACTTGCCCCCCTGGGCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGGGTGGCCTTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTCTTCTCGGCATCGTGGTGGTGGTAACCCGGGGTGGCTGGTTAAGTCTTTTTTTggcgggcgatgtt                                                                                          >  1:190037/1‑243 (MQ=255)
                                                                                                                                                                  aGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGGAACACGCGCTGGCTGGTTAAGTCTTTTTATGGCGGCCG‑TCG‑TTTT‑ACCCCATAacac                                                                         >  1:406282/1‑251 (MQ=255)
                                                                                                                                                                                        tAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGAGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAG‑TCG‑TTGT‑ACCCAATACCACATTGCTACCGCCGCTCTGTATc                                                   >  1:389561/1‑251 (MQ=255)
                                                                                                                                                                                                cTTGCCGCCCTGCTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGACTCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAG‑TCGGTTGT‑ACCCAA‑ACCACCTTACTACCCCTTCTCTTTATCCGCATGCt                                           >  2:43260/1‑251 (MQ=255)
                                                                                                                                                                                                                                       ggTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAG‑TCG‑GTGT‑ACCCAATACCACATTGCTACCGCCGCTCTGTATTGTCATGATTCCCGCCGGGCTGTTTTTAGCAGGTAAGcccaagaggat    >  1:108694/1‑242 (MQ=255)
                                                                                                                                                                                                                                        gTGGTCGAATCTTCCTGGCAGAGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGGGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAGGTGA‑TTG‑‑ACCCAATAACACATTGCTAACGCTGGTATGGATCGTCAGGCTTCCGGCGTGGCTGttattaagagggagaccgcagatgttt   >  2:339941/1‑230 (MQ=255)
                                                                                                                                                                                                                                         tGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCCCGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAG‑TCG‑TTGTTACCCA‑TACCACATTGGTACCCCTGCTCCTTATCGTCATGCTTTCGGCATGGCTGTTGTTAACAGGGAAACCGAAGGTGATgg  >  2:119771/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               
CCACGGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAG‑TCG‑TTGT‑ACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGG  >  NC_000913/2510362‑2510845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑