Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,827	C→T	L152F (CTT→TTT)	evgS →	sensor histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,827	0	C	T	100.0%	31.3 / NA	13	L152F (CTT→TTT)	evgS	sensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/8);  total (5/8)

AGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTA  >  NC_000913/2484600‑2485057                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       agcaACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCtt                                                                                                                                                                                                                 >  1:348713/1‑251 (MQ=255)                                           tAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAACCCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                                                       >  1:44386/1‑251 (MQ=255)                                            aaaaaGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGTTTGCAGAAGGTGAAGTCTATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCTCCTTTCACGACTCAATGCGACCGCTTACCTCTTCAAAACCAGTAAATATTGCACGGTTAGCAATTTAc                                                                                                                                                                       <  2:387257/250‑1 (MQ=255)                                                                                           atcaAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTAtc                                                                                                                       <  1:196317/251‑1 (MQ=255)                                                                                                                         gTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGc                                                                                          >  1:399958/1‑250 (MQ=255)                                                                                                                         gTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACAGTTCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCTCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCATCAATTAGCTCTTTTATAAATTGATATCAGGCATTAGCa                                                                                         <  1:344486/251‑1 (MQ=255)                                                                                                                                    ataGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAg                                                                               <  1:130886/250‑1 (MQ=255)                                                                                                                                         ggtATCACATTTAGTTACTTCGCCGCCACTTAATAATGACATTGCTGCAACCAAACCATTTATAATGACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGGAAACATTGCTCGGGTATCAAATTACCCCCCAGTCGATGTAATTCATCATTCATTGCCAAAAGCATCAATGATCTCTTTGACAAATTTATAGCAGGGATTaccacccggctatgctggtc                                                                         <  2:296778/249‑20 (MQ=255)                                                                                                                                                                              gACATTGCTGCAACCAAACCATTGATAATTACCTTTCCTGCGCTGGAAACCACCTTTCACGACTCAAAGCGACCTCTTACCTCACCAAAACCACTAAATATTTCTCGTGTAGCAAATTACCCCCCATACTAGGTAATTCATCATTCATTGCCAAAAGCAACAATTAt                                                                                                                        <  2:456263/167‑1 (MQ=255)                                                                                                                                                                              gACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCAACTTTCACGACTCAATGCGACCGCTTACCTCACCAAAAACAGTAAATATTGATAGGGTAGCAAATTACCCCCCAGACGAGGTAATTCTTCAATCATTTCCAAAAGCAACAATTAt                                                                                                                        >  1:456263/1‑167 (MQ=255)                                                                                                                                                                                 aTTGCTGCAACCAACCCATTGATAATTACCTTTCCAGCGCTGGTAACCACCTTTCACGACTCAATGCTACCGCTTACCTCACCAGAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAATTTTATATCAGGCATTAGCATCCGTCTAAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcagc                                   >  2:58646/1‑249 (MQ=255)                                                                                                                                                                                                    tGATAATTACCATTCCGGCGATGGTAACCACCTTTCTCGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTTCTCGGGTAGCAAATTACCCCCCAGACTAGGTAATTCATCAAACATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCCCAGCTGGGCACAATTATTACTTTATTGGTAGTAACATCATTACCATCAGTATGATTTCCCGCTAttt               <  2:271889/250‑1 (MQ=255)                                                                                                                                                                                                                 tCCGGCGCTTGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCTCTCACTCCTTa  <  2:403810/250‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       AGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTA  >  NC_000913/2484600‑2485057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑