Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,339,162	C→T	D87N (GAC→AAC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,339,162	0	C	T	100.0%	100.1 / NA	31	D87N (GAC→AAC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/14);  total (17/14)

GTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTC  >  NC_000913/2338926‑2339404                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   gTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCAGTCATAGTTATCAACGAACTCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTCTAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTAACAGCATATAACGCAGCGAGACTGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGt                                                                                                                                                                                                                                      >  2:29533/1‑251 (MQ=255)      gAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAATCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCAcc                                                                                                                                                                                                                                  >  1:302063/1‑250 (MQ=255)                            cGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTa                                                                                                                                                                                                           <  1:159544/251‑1 (MQ=255)                                            ccGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGAATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACg                                                                                                                                                                                           >  1:25934/1‑251 (MQ=255)                                               tCATAGTTATCAACGAAATCGAACGTCTCTTTTTAGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATAGTGTTATAGACCGCCGAATCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGc                                                                                                                                                                                        >  2:92025/1‑251 (MQ=255)                                                   aGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTAGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCAATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGc                                                                                                                                                                                        >  2:438898/1‑247 (MQ=255)                                                                      cGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGCGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGACATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGGTGGTATTTACCGCTTACGTCACCAACGATACGGGCAGCTTTTTTCTAGGCTTTGTTcc                                                                                                                                                                 >  2:228899/1‑251 (MQ=255)                                                                                         gCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGtt                                                                                                                                              >  2:382005/1‑251 (MQ=255)                                                                                                                                  ataACGCATTGCCGCCGCAGAGTCGCCGTCGCTAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAt                                                                                                     >  2:56778/1‑251 (MQ=255)                                                                                                                                   taACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCAtc                                                                                                    >  1:308619/1‑251 (MQ=255)                                                                                                                                            gccgccGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCAATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATc                                                                                           >  1:204884/1‑251 (MQ=255)                                                                                                                                                 cgcAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCa                                                                                      <  1:400960/251‑1 (MQ=255)                                                                                                                                                   cAGAGTCGCCGTCGATAGAACCGAAGTTCCCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAgc                                                                                    <  2:353316/251‑1 (MQ=255)                                                                                                                                                    agagTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTTATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAgcg                                                                                   <  2:133579/251‑1 (MQ=255)                                                                                                                                                    agagTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAgcg                                                                                   >  2:10797/1‑251 (MQ=255)                                                                                                                                                           ccGTCGATAGAACCGAAGTTACACTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACAATGGGGATGGTATTTACCGATTACCTCACCAACTACACGGGAAGATTTTTTATAGGTTATGTTCCAGTCATTGCCTAGTACGTTCATGGAGTAAAGTACGTGACGGTGTACCGGCTTCAGGCCGTCTCGGACATCTGGGAGCGCACGGcc                                                                            >  2:253152/1‑251 (MQ=255)                                                                                                                                                                   ataaCCGAAGTTACCCTGACCGACTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGACGGTATTTACCGATTACGTCGCCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATg                                                                     <  1:463417/248‑1 (MQ=255)                                                                                                                                                                       ccGAAGTTACCCTGACCGTCTCCCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCCCCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGa                                                                <  2:181716/251‑1 (MQ=255)                                                                                                                                                                            gTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCg                                                           <  1:29533/251‑1 (MQ=255)                                                                                                                                                                            gTTACCCAGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGCAGGAATTTACAGATTACGTCAACAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTCGAACGTTCATGGCGTAAAGACCGCGACGGTGTACCGGCTTCAGGCAATCTCGGACATCTGGCAGCGCACGGCCAACACTGACCgcaatcg                                                           >  2:409747/1‑245 (MQ=255)                                                                                                                                                                                    gACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCAGGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTGTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAAt                                                     <  2:286090/249‑1 (MQ=255)                                                                                                                                                                                            aCCAGCGTATAGCGCAGCGAGAATGGCTTCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGa                                           <  1:431071/251‑1 (MQ=255)                                                                                                                                                                                            aCCAGCATATAACGCAGCGAGAATGGCTGCGCCCTGCAGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGCTTACGTCACCAACGACCCGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCCGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAgg                                            >  2:433660/1‑250 (MQ=255)                                                                                                                                                                                            aCCAGCATATAACGCAGCGAGAATGGCGGCGCCATGTGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGAGTACGGCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCTACGGTGTACCGGCTTCAGGCCATCGCGGACATCTGTCAGCGCACGGCCAATAATGACCGACATCGCATAATCCAGATAg                                             <  1:409901/249‑1 (MQ=255)                                                                                                                                                                                               aGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTTAGAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagct                                        <  1:67043/251‑1 (MQ=255)                                                                                                                                                                                                             cGAGAAGGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGGCACCAACGACACGGGCAGATTTTGTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGagctcttcagctcttc                            <  2:116236/249‑1 (MQ=255)                                                                                                                                                                                                                gaATGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTTCGGCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATTATCCAGATAGGAGCTCGTCAGCTCTTCCTCaa                       <  2:25934/251‑1 (MQ=255)                                                                                                                                                                                                                 aaTGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAAt                      >  1:386425/1‑251 (MQ=255)                                                                                                                                                                                                                  aTGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATg                     >  1:439915/1‑251 (MQ=255)                                                                                                                                                                                                                  aTGGCTGCGCCATGCGGACGATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATg                     <  1:11928/251‑1 (MQ=255)                                                                                                                                                                                                                                     gATCGTGTTATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTtctc  >  1:421144/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   GTTAGGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTATTTACCGATTACGTCACCAACGACACGGGCAGATTTTTTATAGGCTTTGTTCCAGTCATTGCCTAGTACGTTCATGGCGTAAAGTACGCGACGGTGTACCGGCTTCAGGCCATCTCGGACATCTGGCAGCGCACGGCCAACAATGACCGACATCGCATAATCCAGATAGGAGCTCTTCAGCTCTTCCTCAATGTTGACCGGTGTAATTTCTC  >  NC_000913/2338926‑2339404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑