Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,329,209	A→G	W197R (TGG→CGG)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,329,209	0	A	G	100.0%	171.5 / NA	53	W197R (TGG→CGG)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/28);  total (25/28)

GCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCG  >  NC_000913/2328965‑2329454                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGtttt                                                                                                                                                                                                                                                 <  1:141582/251‑1 (MQ=255)   cgtcgtACTCTTTGCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCTCCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCa                                                                                                                                                                                                                                               <  1:427401/251‑1 (MQ=255)               ttCCGATGTGAATTTCTTCTGCGGATAACGGCCAACCAGCTACACCCCTACACATTGCCCACTGGCAACCTGCGACGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGACTTGCTGGTGCAAATGCCAGTAACCCACAGCGGTGTACCATCGGTTACCCAGCCAGCAAAACCACCCTGACGAATTTCCTGTTTCTCATCGGCAAAACAGCTCCGGGTTTTCACCCGAACCCGAc                                                                                                                                                                                                                                   >  2:301524/1‑251 (MQ=255)                    cTGTGATTTTCTTAAGCGGATAGCGGGCAAACACCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGGTTTCACCCGTACCCGACTACCg                                                                                                                                                                                                                              >  1:376046/1‑251 (MQ=255)                      gggatgtgcttcAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAg                                                                                                                                                                                                                            <  1:383061/243‑1 (MQ=255)                         aTTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCAt                                                                                                                                                                                                                         >  1:294133/1‑251 (MQ=255)                                     ggATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACAcc                                                                                                                                                                                                             <  2:4905/251‑1 (MQ=255)                                         aGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGAtt                                                                                                                                                                                                         <  2:35718/251‑1 (MQ=255)                                                         cACCTCTCCACATTGCCAACTGGCAACCTGCGTCGGCCCTGGCAAAACCCGATTACGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGAt                                                                                                                                                                                                                     >  2:187746/1‑223 (MQ=255)                                                         cACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGGAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCGCCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGAt                                                                                                                                                                                                                     <  1:187746/223‑1 (MQ=255)                                                         cACCTCTACACATTGCCCACTGGCAACCGGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGGGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCATGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTTCCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGca                                                                                                                                                                                         <  2:274136/251‑1 (MQ=255)                                                                  ccaTTGCCCACTGGCAACCTGCGTCGGCACTGGCAAACCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCTTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCa                                                                                                                                                                                <  2:385333/250‑1 (MQ=255)                                                                   caTTGCCCACTGGCAACCGGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCCCTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACGCCGAGTTTCGCCTCGTCCAGCACCACATCCAg                                                                                                                                                                               <  2:288485/251‑1 (MQ=255)                                                                       gCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCCTAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCACACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATTGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTa                                                                                                                                                                             >  2:357189/1‑249 (MQ=255)                                                                                 aaCCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCta                                                                                                                                                                   >  1:381621/1‑248 (MQ=255)                                                                                      gCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCtt                                                                                                                                                              <  1:266082/249‑1 (MQ=255)                                                                                           ggCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTg                                                                                                                                                       >  2:297213/1‑251 (MQ=255)                                                                                              aCTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGCCGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACCTCCAGTAAGACTTCCTGAGCTTTGTTTTgcgc                                                                                                                                                    >  2:58435/1‑251 (MQ=255)                                                                                                 ggCAAACCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCgg                                                                                                                                                  <  2:8774/250‑1 (MQ=255)                                                                                                       aCCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGt                                                                                                                                           <  1:357189/251‑1 (MQ=255)                                                                                                         ccGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCg                                                                                                                                         >  1:185623/1‑251 (MQ=255)                                                                                                                           aTAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTAGCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTACCTGAGCTTTGTTTTGCGCGGGTAACGGCGCTAATGAATCAAGCAGCg                                                                                                                       <  2:457666/251‑1 (MQ=255)                                                                                                                              aCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTa                                                                                                                                      <  1:144478/233‑1 (MQ=255)                                                                                                                              aCGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTa                                                                                                                                      >  2:144478/1‑233 (MQ=255)                                                                                                                                gggTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGTGAttt                                                                                                                   >  1:370960/1‑250 (MQ=255)                                                                                                                                        aCCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTg                                                                                                                                                       >  2:317880/1‑206 (MQ=255)                                                                                                                                        aCCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTg                                                                                                                                                       <  1:317880/206‑1 (MQ=255)                                                                                                                                        aCCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTAc                                                                                                          <  1:346714/251‑1 (MQ=255)                                                                                                                                                gCTGGTGCCACTGCCCGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGCTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGttt                                                                                                  >  2:66624/1‑251 (MQ=255)                                                                                                                                                gCTGGGGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACGCCGATTTTCGCCTCGTCCAGCACCACAGCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCTAGGtt                                                                                                   <  2:231901/250‑1 (MQ=255)                                                                                                                                                 cTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTc                                                                                                 >  2:373419/1‑251 (MQ=255)                                                                                                                                                              cAGTAACCCACAGCGGAGTACCATCGGTTAATCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGAATACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTCACGTCGCTAATGAATCCAGAAGCGTTTTAACCGTTACCGAGGTTTATGGCTTCAGCGt                                                                                     >  2:10814/1‑250 (MQ=255)                                                                                                                                                               aGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAg                                                                                   <  1:232705/251‑1 (MQ=255)                                                                                                                                                                   aCCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTGCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTGTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCTATTTATCCGTTTCCGATGTTTCTTGCTTCAGCGTAGTCag                                                                               <  2:294133/251‑1 (MQ=255)                                                                                                                                                                         aGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCCGCACCACATCCAGTAAGACTTCCTGAGCTGTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTGAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGc                                                                         >  2:329104/1‑251 (MQ=255)                                                                                                                                                                                  ccATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGc                                                                >  1:74625/1‑251 (MQ=255)                                                                                                                                                                                       ggttaaaaagcaagcACAACCACCCTGACGGCTTTCCTGCTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGAATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTAGCTAATGAATCAAGCAGCGATTTAACAGTTACCGAGGTTTCTGGCTTAAGCGTAGACAGAGACGCTAACCACGCAGGTg                                                           >  2:284366/13‑251 (MQ=255)                                                                                                                                                                                             ccagccagCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGAc                                                     <  2:185623/251‑1 (MQ=255)                                                                                                                                                                                                    gcAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTg                                              <  1:297213/251‑1 (MQ=255)                                                                                                                                                                                                        aaCCACCCTGACGGATTTCCTGTGTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCTTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAg                                          <  1:226176/251‑1 (MQ=255)                                                                                                                                                                                                                      aTTTCCTGTTTATCATCGGCAAACCAGCTACGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGCCACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCcacac                            >  1:367924/1‑251 (MQ=255)                                                                                                                                                                                                                         tCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAAt                                                                                                                                    >  1:108102/1‑144 (MQ=255)                                                                                                                                                                                                                         tCCTGTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAAt                                                                                                                                    <  2:108102/144‑1 (MQ=255)                                                                                                                                                                                                                             gTTTATCATCGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACCTCTgcg                     >  2:124313/1‑251 (MQ=255)                                                                                                                                                                                                                              tttATCATCGGCAAACCAGCTCCGGGTTTTCAACCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTgg                                                                                              >  1:338687/1‑177 (MQ=255)                                                                                                                                                                                                                              tatATCAACGGCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGTGCTTGGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCTATTTAACCTTTACCGAGGTTTCTgg                                                                                              <  2:338687/175‑1 (MQ=255)                                                                                                                                                                                                                                 atcatcGGCAAACCAGCTCCGTGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCa                  <  1:45206/250‑1 (MQ=255)                                                                                                                                                                                                                                       ggCAAACCAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCTCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGGTGCCAGTATTGCCCCCACACATCTGCGTCGATATGCa           <  2:190644/251‑1 (MQ=255)                                                                                                                                                                                                                                              cAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTg    <  1:318413/251‑1 (MQ=255)                                                                                                                                                                                                                                              cAGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTg    >  2:379554/1‑251 (MQ=255)                                                                                                                                                                                                                                               aGCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTgc   >  1:86634/1‑251 (MQ=255)                                                                                                                                                                                                                                                gCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCGCCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTgcg  <  1:379022/251‑1 (MQ=255)                                                                                                                                                                                                                                                gCTCCGGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTgcg  >  2:17146/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGCACTGGCAAAACCCGATTAAGCACCGTCGCATAACGGGTTAGTACCGTTTTGCTGGTGCCACTGCCAGTAACCCACAGCGGTGTACCATCGGTTAACCAGCCAGCAAAACCACCCTGACGGATTTCCTGTTTATCATCGGCAAACCAGCTCCAGGTTTTCACCCGTACCCGACTACCGAGCATGGATGCGACACCGATTTTCGCCTCGTCCAGCACCACATCCAGTAAGACTTCCTGAGCTTTGTTTTGCGCGGGTAACGTCGCTAATGAATCAAGCAGCGATTTAACCGTTACCGAGGTTTCTGGCTTCAGCGTAGTCAGAGACGCTAACCACGCAGGTGCCTGACGCTGTTGCCAGTATTGCCCCCACACATCTGCGCCAATATGCAACCGCTGCG  >  NC_000913/2328965‑2329454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑