Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,137,543	G→A	intergenic (‑300/‑359)	wza ← / → yegH	outer membrane polysaccharide export protein Wza/inner membrane protein YegH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,137,543	0	G	A	100.0%	59.4 / NA	18	intergenic (‑300/‑359)	wza/yegH	outer membrane polysaccharide export protein Wza/inner membrane protein YegH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/12);  total (6/12)

CGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGTTTTATTATTACCACTTTTAATTCAGGG  >  NC_000913/2137299‑2137775                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         cGCCATGCACATTTCCCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCAGCCGCAGGCTATTGACAGAATAATTCAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACa                                                                                                                                                                                                                                    <  1:391650/251‑1 (MQ=255)     aTGCACATTTACCATGCGGTTAATTAAATAAAAAATTAACTGGCATCAGGCCAAAAAAAATTGATTTCACCCTAAGCCTCGTGACAGAATAATTCAGACTGGTCTTTCATGCATCCGGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTTACTTCCGAATATAAACTGACATTAt                                                                                                                                                                                                                                <  2:166108/251‑1 (MQ=255)               cccTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGaa                                                                                                                                                                                                                      <  1:391081/250‑1 (MQ=255)                                               gCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAgtgt                                                                                                                                                                                      >  1:129284/1‑251 (MQ=255)                                                                              cAGGCTATTGACAGAATAATTAAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAAc                                                                                                                                                        >  1:105635/1‑250 (MQ=255)                                                                                    aTTGACAGAATAATTCAGCCTGGGTTTTCAGGCATCCAGCCACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTc                                                                                                                                                   <  1:218961/249‑1 (MQ=255)                                                                                                                                  aCCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTGTTCACACCCTCTCAGCAAGCAGTCGTTGATGAGAAAGGGTTATTACGTAAATTAACTTCCGAATAGAAAGTGACATTATGGTAATTGAACATTGGCTTTCCAATAATGCAGGAGGAAGTGTTTCAGCTAACGGAATAGCAGGGAAGAGAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATgg                                                                                                    <  2:448442/250‑1 (MQ=255)                                                                                                                                                tttAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTc                                                                                      <  2:105635/250‑1 (MQ=255)                                                                                                                                                    gCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAg                                                                                 <  1:278544/251‑1 (MQ=255)                                                                                                                                                                cTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATAtt                                                                     >  1:280596/1‑251 (MQ=255)                                                                                                                                                                            aaTTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAgtgt                                                          <  2:407797/250‑1 (MQ=255)                                                                                                                                                                              tttttCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGtctc                                                       >  1:404649/1‑251 (MQ=255)                                                                                                                                                                                 ttCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTTACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTATTAGGAGTTTCTTTAGGTTGACAATATTTAATATAAGGTCTCca                                                     <  2:432433/250‑1 (MQ=255)                                                                                                                                                                                               gCATGCAGTCGATGATGAGACAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATCTAGTGTCTCCACATGCGATATTTCtt                                      >  2:55747/1‑251 (MQ=255)                                                                                                                                                                                                        cGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATg                              <  1:91853/250‑1 (MQ=255)                                                                                                                                                                                                         gTTGATGAGAAAGGGTTATTTCGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGtt                            <  2:327211/251‑1 (MQ=255)                                                                                                                                                                                                                             aCGGAAATTAACTTCCGAATATAAAGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAAGATGGTGTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCGAGATGCGATATTTCGTAAATAATGTTTTATTATTACCACTTTTaa         <  1:194632/250‑1 (MQ=255)                                                                                                                                                                                                                                    ttAACTTCCGAATATAAAGTGACATTATGGCAATTGAATATTTGCTATCCCATAATGAAGGAGGAAGTGTTACAGCTAACGGAAAAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGAGTAATTCAATATGGTTTTTCGGAGTTTCTTTTGGTTGACAATATTTAATATAGTGTCTCCACCTGCGATATTTCTTAAATAATGTTTTATTATTACCACTTTTAATTCAggg  >  2:214070/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         CGCCATGCACATTTACCTTGCAGTTAATTGAATAAAAATTTAACTGGCATCAGTCCTAAAAAAATTGATTTCATCCGCAGGCTATTGACAGAATAATTCAGACTGGTCTTTCAGGCATCCAGACACGCTACCGCCCCTGGCTTTTTAGCTACCAATACACTGATTTAGTTTAATTTTTCACACCCTCTCAGCATGCAGTCGTTGATGAGAAAGGGTTATTACGGAAATTAACTTCCGAATATAAGGTGACATTATGGTAATTGAATATTGGCTTTCCAATAATGCAGGAGGAAGTGTTACAGCTAACGGAATAGCAGGCAAGATAACAATTCGGTAATTGGCTATTTTTAAGAATTATATTAAGTGTCATTCAATATGGTTTTTAGGAGTTTCTTTAGGTTGACAATATTTAATATAGTGTCTCCACATGCGATATTTCTTAAATAATGTTTTATTATTACCACTTTTAATTCAGGG  >  NC_000913/2137299‑2137775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑