Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,122,908	G→A	I24I (ATC→ATT)	cpsG ←	phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,122,908	0	G	A	100.0%	83.5 / NA	26	I24I (ATC→ATT)	cpsG	phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/14);  total (12/14)

GCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCGATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCGAGATAAGAGCCGG  >  NC_000913/2122662‑2123145                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              gccattATAAGCCATCGGATTATGGCTGGCGGTAACTGCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAatat                                                                                                                                                                                                                                           <  2:106190/247‑1 (MQ=255)   cGTTATAATCCACCTGGTTATGGATGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGGTCTCTTCGGTGCCGGACATACCAATATCCAGCACGGCAACGCCCGCATCCTGTAAACCTGTCGCCAGCGCCAGTTTTAAGGTTTCGGGGGTGAGGCGGACATCACCGCCTAACACGATGGTTGTCGGGGTGAGAAAGTCGCCATAGGCGCGACCTATGCGCCAGGCAATATCt                                                                                                                                                                                                                                         <  2:209212/251‑1 (MQ=255)       ataATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCttcat                                                                                                                                                                                                                                     >  2:68170/1‑251 (MQ=255)                                                         cGAGATGGATCGTGGCGAACTCGATCTCTTCGGTGCCGGACATCCCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGACTATCATAGGCTTTAAAGCAGGtt                                                                                                                                                                                   >  2:73652/1‑251 (MQ=255)                                                           agaTGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTaa                                                                                                                                                                                 <  1:424744/251‑1 (MQ=255)                                                             aTGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAAtt                                                                                                                                                                               <  1:464861/251‑1 (MQ=255)                                                               ggAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAAtttt                                                                                                                                                                             >  2:352382/1‑251 (MQ=255)                                                                        cGAAATAGATCTCTTCGGGGCCGGACATACCAATATCCAGCACGTCAACGCCCGTATCCTGTAAACCTTTCGCCAGCGCCAGTTTGAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCGAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCg                                                                                                                                                                    <  1:15942/251‑1 (MQ=255)                                                                                         gTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCa                                                                                                                                                   <  2:160216/251‑1 (MQ=255)                                                                                           gCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTGTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCaa                                                                                                                                                  <  2:404405/250‑1 (MQ=255)                                                                                                   tACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGcc                                                                                                                                         >  1:257225/1‑251 (MQ=255)                                                                                                    aCCAATATCCAGCAAGTCAACGAACGAATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAAGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCACCCAATGCGCAAGGCAATATCTTCTTTCAGTTCTTCGCCTAATTTCCCGCGAAGATCCTAGGCTTTAAAGCAGTTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTggacc                                                                                                                                        >  2:306945/1‑248 (MQ=255)                                                                                                              aGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGc                                                                                                                              <  2:392372/251‑1 (MQ=255)                                                                                                                                        cccTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTAttt                                                                                                    <  2:133651/250‑1 (MQ=255)                                                                                                                                            ttCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTacac                                                                                                >  2:132182/1‑251 (MQ=255)                                                                                                                                                        agTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACCATGGTTTTCGGTTTGAGAACTTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTCTTTTTGTCGTTATTTACACCGCGGTTTCGc                                                                                     >  2:43275/1‑250 (MQ=255)                                                                                                                                                        agTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGca                                                                                    <  1:342540/251‑1 (MQ=255)                                                                                                                                                              aaGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCAGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGcattcatt                                                                              >  1:94561/1‑251 (MQ=255)                                                                                                                                                                 gTTTCGCTGGTGAGGCGGACAGCACCGCCTAACACAAGGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTACATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGcc                                                                           <  2:240997/251‑1 (MQ=255)                                                                                                                                                                      gCTGGTGAATCGGCCATCATCGCCTAGAACACTAGTTTTCGTTTTGAGAAATTAGCCATAGCCTCAACCAATGCGCCAGGCAATATCATAATTCAGTTCTTCGCCTAATTCACAGCGAATATCATCGACATTAAACTAGGTTATTTCTTTACTATCGTTACCCTTTTTCAGGCAATAGTTGGACCTGACCGAAGCGGACAATTTTACTTTTGTCGCTATTTACACCCCGGTTTCCCATTCATTGCCTAATg                                                                      >  2:305296/1‑251 (MQ=255)                                                                                                                                                                               gCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTa                                                             <  1:73652/251‑1 (MQ=255)                                                                                                                                                                                            ccgaaCACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAg                                                <  2:446324/248‑1 (MQ=255)                                                                                                                                                                                                                   gagaAATTCGCCATAGGCGCGACCAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCAcatc                         >  2:174398/1‑251 (MQ=255)                                                                                                                                                                                                                                          cAATGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTACCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCGAGATAAGAGACgg  >  2:133256/1‑251 (MQ=255)                                                                                                                                                                                                                                            aTGCGCCCGGCAGTATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATCGGCTTTAAAGAAGGCTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATCGTTGGCCCTGACCGAAGCGGCCa                                                                                                                         >  2:202740/1‑130 (MQ=255)                                                                                                                                                                                                                                            aTGCGCCAGGCAATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCa                                                                                                                         <  1:202740/130‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              GCCGTTATAATCCATCGGATTATGGCTGGCGGTAACTTCAATGCCGCCATCCACGCCGAGATGGAACGTGGCGAAATAGATCTCTTCGGTGCCGGACATACCAATATCCAGCACGTCAACGCCCGCATCCTGTAAACCTTTCGCCAGCGCCAGTTTTAAGGTTTCGCTGGTGAGGCGGACATCACCGCCTAACACAATGGTTTTCGGTTTGAGAAATTCGCCATAGGCGCGACCAATGCGCCAGGCGATATCTTCATTCAGTTCTTCGCCTAATTTCCCGCGAATATCATAGGCTTTAAAGCAGGTTAATTTTTTCATATCGTTACCCTTTTTCAGGCAATAGTTGGCCCTGACCGAAGCGGCCAATTTTATTTTTGTCGTTATTTACACCGCGGTTTCGCATTCATTGCCTGATGCGACGTTTACACCCGTCCGTAGCGATCCGCGAAACGCACCACATCATCCTCTTCGAGATAAGAGCCGG  >  NC_000913/2122662‑2123145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑