Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,103,891	G→A	P207S (CCA→TCA)	wbbK ←	putative glycosyltransferase WbbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,103,891	0	G	A	100.0%	40.5 / NA	14	P207S (CCA→TCA)	wbbK	putative glycosyltransferase WbbK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/10);  total (4/10)

CCTAGAGGGAAAACAAACTATATCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATA  >  NC_000913/2103664‑2104106                                                                                                                                                                                                                                    |                                                                                                                                                                                                                        ccTAGAGGGAAAACAAAGTATATCTGAACTATTATAACAACGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCTTTCTGCAAGACTGATAATATATTTTGCATAAGCATTTTCTGTACAACTGATCGTAAGCAGAAATTTAATATTGGATTGTTCTTTCATTTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTc                                                                                                                                                                                                   >  2:466362/1‑250 (MQ=255)             acaaacTATATCTGAAATATTATAACAATGAGCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTTCCACTGAGAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTTCTGCACTAATAATAAGCTCGTAAGTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGt                                                                                                                                                                                      <  1:466362/251‑1 (MQ=255)              caaacTATATCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCGTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGtt                                                                                                                                                                                     <  1:2423/251‑1 (MQ=255)                                       aaTGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAAtcatcatca                                                                                                                                                            <  1:157370/251‑1 (MQ=255)                                        attatctaTTTTTTCTTTATCCAAGTTCCCGAGGAAATCAACATTACCCACGCCTTCGGGAAGACTGATAATATATTTTTCATACTCATTTTCTGTACCTCTTTTAGTAAGCAGAAATTTAATATTTGATTTTTCTTTCAATTTCCTTACTGCACTAATAATAAGCTCGTAATTTTTAAATTATCGAGAAACAGCAGGGTAAAATATTGTTATCTCAGAAGGTGTGTTCGTAAATTGACGCTCATCATCAg                                                                                                                                                           <  2:258475/244‑1 (MQ=255)                                          gATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAAcag                                                                                                                                                                                                                    >  1:215366/1‑192 (MQ=255)                                          gATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAAcag                                                                                                                                                                                                                    <  2:215366/192‑1 (MQ=255)                                           aTCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTaa                                                                                                                                                        <  2:140719/251‑1 (MQ=255)                                                                               aaCATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGAc                                                                                                                    <  1:18751/251‑1 (MQ=255)                                                                                                                                         ccACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAAttg                                                          >  1:18890/1‑251 (MQ=255)                                                                                                                                              gATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGCTAAATTTTTCTTTCATCCAGAATTGTTGa                                                      <  2:353436/250‑1 (MQ=255)                                                                                                                                                                aaTATTGGATTGTTCTTTCCATTTCCTTGCTGCACTAATATTAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAACTATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTAttt                                    >  2:406769/1‑250 (MQ=255)                                                                                                                                                                aaTATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGAGAAATTTTTCTTGCATCCAGAATTGTTGAACAAACTCTGCAGTAttt                                    <  1:406769/250‑1 (MQ=255)                                                                                                                                                                                                 aCTAATAATAAGCTCGTAATTTTTAAATACTCGTGAAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCTGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCTCGtata  <  2:324957/251‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                        CCTAGAGGGAAAACAAACTATATCTGAAATATTATAACAATGATCGATTTTTTCTTTATCCAAGTACCCGAGGAAATGAACATTATCCAGTCCTTCTGCAAGACTGATAATATATTTTGCATACGCATTTTCTGTACCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATA  >  NC_000913/2103664‑2104106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑