Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,086,634	G→A	A143A (GCC→GCT)	plaP ←	putrescine:H(+) symporter PlaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,086,634	0	G	A	100.0%	130.2 / NA	38	A143A (GCC→GCT)	plaP	putrescine:H(+) symporter PlaP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/25);  total (13/25)

CCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAGGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTAAACAGGAT  >  NC_000913/2086390‑2086879                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      ccgacaaattgatGATGCCGTCCACGCCGGTCAATGCGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGACTGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCAACGCGATCAGCACTACCTGCCACACGCCGATTACAGTGTTGAAGTTCGATACGGCTTTCAGACTACGCAGGTTAAAAGCGGTc                                                                                                                                                                                                                                                 >  2:283925/13‑251 (MQ=255)   gACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACAGGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCAt                                                                                                                                                                                                                                               <  1:428823/251‑1 (MQ=255)    aCAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATa                                                                                                                                                                                                                                              <  1:189150/251‑1 (MQ=255)      aGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATaaa                                                                                                                                                                                                                                            <  1:384458/251‑1 (MQ=255)      aGGTTGCTGATGCCGGCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAAAACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTGCAGACTACGCAGGTTAAAAGCGTTCATaaa                                                                                                                                                                                                                                            <  2:288815/251‑1 (MQ=255)                      cAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCATACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGccac                                                                                                                                                                                                                             <  1:448975/250‑1 (MQ=255)                      cAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGACCAGGTAATCAGCGGGATAAAATGCGCATCTCCAGACCAGAATGGACGAGGGCTCGCCAGCGTACCAGCGCCTTCACCTGCAAATACGCCATAACCAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGACTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGTCCACCAGCGccacc                                                                                                                                                                                                                            <  2:284183/251‑1 (MQ=255)                        aaGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCcaccac                                                                                                                                                                                                                          >  2:95755/1‑251 (MQ=255)                                         gCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACgatggga                                                                                                                                                                                                         <  1:6745/251‑1 (MQ=255)                                           acaGGATTGTCGCCCCGGGAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCTACgatgggatg                                                                                                                                                                                                       >  2:60875/1‑251 (MQ=255)                                                    gcGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCAcc                                                                                                                                                                                              <  2:159935/250‑1 (MQ=255)                                                       ccccGGTAATCATCGGGATAACAGGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGTTTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCaga                                                                                                                                                                                           <  2:92909/251‑1 (MQ=255)                                                                      ggATAACATGCGCATCGCCAGACCAGAATCGACGAGTGCACGCCAGCGTTCCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCCCTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACCACGCAGGTTAAAAGCGGTCATAAAGGCCCCCAGCGCCACCACAAACATCCACGATTGGATGGAAGGCACCAGAGCTTCAAAATAGAtt                                                                                                                                                                            <  1:39777/251‑1 (MQ=255)                                                                        aTAACATGCGCATCGCCAGACCAGCATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCTATACCTGCATCTCGACTATTACAGTGTTGAAGTTCGCTACGGATTTCATACCACGCAGGTTAAAAGCGGTCATAAAGGCCACCATCGCCACCACAATCATCCACGATGGGATCGAAGGCACCAGAGCTTCAAAATAGAttta                                                                                                                                                                          <  2:249437/251‑2 (MQ=255)                                                                        aTAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGAtttt                                                                                                                                                                          >  1:457759/1‑251 (MQ=255)                                                                             aTGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCa                                                                                                                                                                     <  1:246040/251‑1 (MQ=255)                                                                                  caccgctataccagaATGGACGAGTGCTCGCCATCGTACCAGCGCCTTCACCTGCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTGGAAGTTCGCTACGGCTTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCCCCAGCGCAACCACAAACATCCACGATGGGATGGAAGGCACCTTAGCGGCAAAAGAGACTTTCGCCAGTAGa                                                                                                                                                                <  2:391772/242‑1 (MQ=255)                                                                                           accagaATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCGTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGGTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCAACACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAAGAGATTTTCGCCAGTAGAATGTTGagc                                                                                                                                                       <  2:446064/251‑3 (MQ=255)                                                                                                                     ggaCCAGCGCCTTCACCTTCAAACACGCCATAAACAACCATGCCCAGAATCACCGCGATCTGCACTTCCTGCAACACTACGATTACAGGGTTGAAGCTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCag                                                                                                                             <  1:325190/249‑1 (MQ=255)                                                                                                                        ccAGCGCCTTCTCCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCag                                                                                                                             >  1:114079/1‑248 (MQ=255)                                                                                                                                  caccttcaAATACGCCATAAACAACCATGCCCAGAATCCCCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGGCGAGCag                                                                                                                             <  2:41186/238‑1 (MQ=255)                                                                                                                                  caccttcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCag                                                                                                                             >  1:41186/1‑238 (MQ=255)                                                                                                                                       tcaAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACccacc                                                                                                             <  1:388351/249‑1 (MQ=255)                                                                                                                                             aCGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGcc                                                                                                     >  1:248343/1‑251 (MQ=255)                                                                                                                                              cGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCg                                                                                                    <  1:177221/251‑1 (MQ=255)                                                                                                                                                    aaaCAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGt                                                                                               <  1:284126/250‑1 (MQ=255)                                                                                                                                                     aacaacCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCACCCCACCATAAAGCCGACAGTCg                                                                                             >  1:183361/1‑251 (MQ=255)                                                                                                                                                         acCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCt                                                                                         <  2:457759/251‑1 (MQ=255)                                                                                                                                                                             gATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCAACAGCGCCACCACAACCATCCACGATGGGATGGAAGGCACAAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCCCCATAAAGCCGACAGTCTGGCTACTGGATTTCTGGGCGTa                                                                       >  1:34373/1‑249 (MQ=255)                                                                                                                                                                              aTCAGCACTACCTGCAACACGACTATTACAGTGTTGAAGTTCGCTACGGAGTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGTCCTCCAGCGCCACCACAAACAGCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGGCGAGCAGATAAGACCAACCCACCATAAAGCCGACAGGCGGTCTAATGGACTTCTGTGCGTAAgt                                                                    <  2:183361/251‑1 (MQ=255)                                                                                                                                                                                 aGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTa                                                                 <  1:95755/251‑1 (MQ=255)                                                                                                                                                                                  gcgcTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCTCAAACATCCACGATGGGATGGAAGCCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTAt                                                                <  2:226203/248‑1 (MQ=255)                                                                                                                                                                                           gCAACACGACGATTACAGTGTTGAAGTTCTCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGc                                                         <  2:248343/249‑1 (MQ=255)                                                                                                                                                                                            cAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTAGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGcc                                                        >  1:461276/1‑249 (MQ=255)                                                                                                                                                                                                     gaTTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGACGACCAACCCACCATAAAGCCGAAAGTCGGGCTAATGGATTTCTGGGAGTAAGTGTATGCAGAGCCAGCAGAAGGAt                                             >  2:413809/1‑251 (MQ=255)                                                                                                                                                                                                           aGTGTTGAAGTTCGCTACGGATGTCAGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGAGCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAGCCCACCATAAAGCCGACAGTCGGGCTAATGTATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAgcggc                                       <  1:239764/251‑1 (MQ=255)                                                                                                                                                                                                                                    aGACTACGCAGGTTAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGc              >  1:88596/1‑251 (MQ=255)                                                                                                                                                                                                                                                ttAAAAGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGGATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCCAAGAGCCGTAAACAGGAt  >  2:372444/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATTTCAGACTACGCAGGTTAAAGGCGGTCATAAAGGCCACCAGCGCCACCACAAACATCCACGATGGGATGGAAGGCACCAGAGCTTCAAAATAGATTTTCGCCAGTAGAATGTTGATCATCGGCGCGAACAGATAGTCGAGCAGAGAAGACCAACCCACCATAAAGCCGACAGTCGGGCTAATGGATTTCTGGGCGTAAGTGTATGCAGAGCCAGCAGAAGGATAGCGGCGAACCAGCTTCCCGTAGCTCAGAGCCGTAAACAGGAT  >  NC_000913/2086390‑2086879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑