Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	246,747	G→T	S12S (TCG→TCT)	yafL →	NlpC/P60 family protein YafL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	246,747	0	G	T	100.0%	218.7 / NA	66	S12S (TCG→TCT)	yafL	NlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (42/24);  total (42/24)

TGGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGC  >  NC_000913/246503‑246983                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             tGGGGTATCTCCTGTGCTGTATATTTAGTCAGCTTGAATTGTAGCACAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGAtt                                                                                                                                                                                                                                         <  1:266538/250‑1 (MQ=255)      tATCTCCTGTGCTGTCTATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTATTTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGtt                                                                                                                                                                                                                                   >  2:390065/1‑251 (MQ=255)                gCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGAtttgtttg                                                                                                                                                                                                                         >  2:113602/1‑251 (MQ=255)                 cTGTATATTTGTTCAGCTAGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATATCATAAACGGATGTTTTTACATAATTAAATGAAATCTATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGc                                                                                                                                                                                                                        >  2:296002/1‑251 (MQ=255)                                                        aaCAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATAtct                                                                                                                                                                                 >  1:148533/1‑251 (MQ=255)                                                            gCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCGTCATTTGCCAGCGCCACCACATCACATATCTCttt                                                                                                                                                                             <  2:408612/251‑1 (MQ=255)                                                               aCAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTAATT‑AAGTTTGTTCAGAAACGATCCGGGATACTCTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTTTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAg                                                                                                                                                                          <  2:120537/251‑1 (MQ=255)                                                                       tGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGCTCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTAcgccg                                                                                                                                                                  >  1:220916/1‑251 (MQ=255)                                                                            aaTTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGccgcccgc                                                                                                                                                             >  1:83010/1‑251 (MQ=255)                                                                               ttAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCAATTATTGCGTATGTCCTTGCCGTCGATTCCCTCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCATCGCCACCACCTCACATCTCTCTTTTCGCTACGCcgcccgcctg                                                                                                                                                          >  1:21063/1‑249 (MQ=255)                                                                                taataaACGGATGTTTTTACATAATTAACTGCAATATATATGTTAATTTTATCATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCAATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGc                                                                                                                                                         >  1:276816/1‑251 (MQ=255)                                                                                  cagttaattatgttttACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACTCCGCACGCCAGCgg                                                                                                                                                       >  2:276419/13‑251 (MQ=255)                                                                                    aaaCGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGAt                                                                                                                                                     >  2:123328/1‑251 (MQ=255)                                                                                      actgATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACGTCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGc                                                                                                                                                   <  1:237834/248‑1 (MQ=255)                                                                                        ggATGTTTTTACATAATTACCTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTACTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACTCCGCCCGCCAGCGGATGCa                                                                                                                                                  >  2:75205/1‑250 (MQ=255)                                                                                            gTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAAcc                                                                                                                                             >  1:351446/1‑251 (MQ=255)                                                                                                ttACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCAATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAACCCGTgc                                                                                                                                         >  1:173540/1‑251 (MQ=255)                                                                                                              gAAATATATATTTTAATTTTATAATAAGGGTTTATG‑AAGTGTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTGGTTTGCCTTTTTCTTCATTTGCCAGCGCCACCATATCACATATCTCTGTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTgcg                                                                                                                                        <  2:222713/238‑1 (MQ=255)                                                                                                              gAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTgcg                                                                                                                                        >  1:222713/1‑238 (MQ=255)                                                                                                                   atatatTTAAATTTTATAATAATGGTT‑ATTGAAGTTTGTTAAGAAACGTTCCGGATTACA‑TGCAAACATAAAATCATGACCTGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTTTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGACCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTa                                                                                                                       <  2:41363/250‑1 (MQ=255)                                                                                                                          ttAATTTTATAATAATGGTTTATT‑AAGTTTTTCCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACt                                                                                                                <  2:31203/250‑1 (MQ=255)                                                                                                                             aTTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATc                                                                                                            >  1:460226/1‑251 (MQ=255)                                                                                                                                 tataATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGa                                                                                                         >  1:169497/1‑250 (MQ=255)                                                                                                                                  atactaatGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGCTACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAACCAGTACCAAACTCATCTGaa                                                                                                        >  2:114789/5‑250 (MQ=255)                                                                                                                                  ataataATGGTCTATT‑AAGTTTGTTCCGAAACGATACGGGATACACTACCA‑CATTAAAGCATGACCTGCCCTTATTGAGTCTGTCCTTGCCGTCGTTTCCATCATTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCCGCTACGCCGCCCGCCAGCGGCTGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaa                                                                                                        >  2:193517/1‑250 (MQ=255)                                                                                                                                  ataaaaATGGTTTATT‑AATTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCAGGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGaaa                                                                                                       <  2:106454/251‑1 (MQ=255)                                                                                                                                     ataatTGTTTATA‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTTTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTGGCCAGCGCCACCACATCACAAATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTGTAGTAAAACAGGACCAAACGCAGCTGAAAAAg                                                                                                    <  2:220916/251‑1 (MQ=255)                                                                                                                                      taatGGTTTATT‑AAGTTTGTTCAGAAACGCTCCGGGATACACTGCAA‑CATTAAAGCATGCCCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAACCTCATCTGAAAAAGc                                                                                                   >  2:384513/1‑251 (MQ=255)                                                                                                                                       aatGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATAGCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCa                                                                                                  <  1:276419/251‑1 (MQ=255)                                                                                                                                           gTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGcc                                                                                              >  1:69759/1‑251 (MQ=255)                                                                                                                                            tttATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGcc                                                                                              >  2:246085/1‑250 (MQ=255)                                                                                                                                             tttaatacgTTTGTTCAGCAACGCTCCGGGATACACTGCAA‑CATTAAAGCATGACCAGTCATTATTGAGTATGTCCTTGCCGTAGATTCCCTCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCAACCACATCACATATCTCTTTCAGCTACGCCGCACGCCAGCGGATGCACAACCGTGCGCGTTTATTAATACAGTACCAAACTCATCTGCAAAAGCAggac                                                                                              >  2:291333/9‑248 (MQ=255)                                                                                                                                             ttatt‑aAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAAATCATCTGAAAAAGCAGGcc                                                                                              >  2:331679/1‑249 (MQ=255)                                                                                                                                               att‑aAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCCAACCCGTGCGCGTTTATTAAACCAGTACCAAACTCATCTGAAAAAGCAGGCCAGCt                                                                                          >  2:324806/1‑251 (MQ=255)                                                                                                                                                   aaGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCtat                                                                                        >  1:383531/1‑250 (MQ=255)                                                                                                                                                   aaGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCtat                                                                                        >  1:38897/1‑250 (MQ=255)                                                                                                                                                          ttCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTgg                                                                                 >  1:365092/1‑250 (MQ=255)                                                                                                                                                              gAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGCTTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACCTCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCACACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGc                                                                            >  1:452882/1‑251 (MQ=255)                                                                                                                                                              gAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGc                                                                            >  2:134051/1‑251 (MQ=255)                                                                                                                                                               aaaCGATCCGGAATCCACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCATTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAACAGCAGGCCAGCTATATTGTGGAAGGc                                                                            >  2:411433/1‑250 (MQ=255)                                                                                                                                                                   gATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTACCGTATTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATATGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGc                                                                       >  1:461449/1‑251 (MQ=255)                                                                                                                                                                    aTCCGGGATACACGGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTGTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGcc                                                                      <  2:358296/251‑1 (MQ=255)                                                                                                                                                                        gggATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGaa                                                                   >  1:266289/1‑250 (MQ=255)                                                                                                                                                                         gtaTACACTGCAA‑CAGTAAAGCATGTCTATCCATTAGTGAGTATGTCCTTGCCTTCGATTCCATCCTTTAGATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTGGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAg                                                                 <  2:367905/249‑1 (MQ=255)                                                                                                                                                                         ggATAAACTGCCA‑CATTGACCCATGACCAGCCGTTATTGAGAATGTCCTTGACGTCGCTTTAATCCTTTGTATAGTCTGGATTCCTGTTGATTTGTTTGCCGTTTTCTTCATTTGCACGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCACGCCCGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGACAGCTATATTGTGGAAGGCAATGCCGaa                                                                   >  2:455090/1‑249 (MQ=255)                                                                                                                                                                              cacTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCaaa                                                             >  2:443726/1‑250 (MQ=255)                                                                                                                                                                              aacctgaaacctaaaaGCAGGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCaa                                                              <  2:383531/237‑1 (MQ=255)                                                                                                                                                                                        aTTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATAtc                                                                                                                                                                                  >  2:92666/1‑124 (MQ=255)                                                                                                                                                                                        aTTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATAtc                                                                                                                                                                                  <  1:92666/124‑1 (MQ=255)                                                                                                                                                                                         ttAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACg                                                  >  2:357219/1‑251 (MQ=255)                                                                                                                                                                                          aaaataaTGACCAGCCATTATTGAGTATTTCTTTGCATTCGATCCCATCCTTTGTATTGTCTGTATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCAAGCGCCCCCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACg                                                  <  2:445833/244‑1 (MQ=255)                                                                                                                                                                                                  gACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGcaca                                          <  1:134051/250‑1 (MQ=255)                                                                                                                                                                                                      ggcctttattGAGTATTCCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTCCCGTTTTCTTCATTTGCCAGGGCCACCACATCCCATATTTCTTTCATCTACGCCGCCCGCCAGCGGATGCAAATCCGTGCTCGTTTATTAAAACAGTACCAAACTCATCTGAAAAATCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCgg                                     <  2:426882/246‑1 (MQ=255)                                                                                                                                                                                                      aGCCATTCTTGAGTAAGCCATTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTAACAAACt                                                                                                                >  2:421854/1‑176 (MQ=255)                                                                                                                                                                                                      aGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATACCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACt                                                                                                                <  1:421854/176‑1 (MQ=255)                                                                                                                                                                                                       gCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTa                                                                                                                       >  1:170625/1‑168 (MQ=255)                                                                                                                                                                                                       gCCATTATTGAGTATGTCCTGGCCGTCGATTCCATCCTTTGTATGGTCTGGAGTCCTGTTGATTGGTTTGCCGTTTTCTTCATTTGCCAGCGCCCCCACATCACATATCTCTTACAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTa                                                                                                                       <  2:170625/168‑1 (MQ=255)                                                                                                                                                                                                         aattattGAGTATGTCCTTGCCGTTGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTGTTAAAACAGTACCAAACTCATCGGAAAAAGCAGGCCAGCTATAGTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAg                                  <  1:220221/250‑1 (MQ=255)                                                                                                                                                                                                            tattGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCGCATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCCAAACAGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAACGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGc                                 >  1:228089/1‑249 (MQ=255)                                                                                                                                                                                                                   tATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAAc                        >  1:465981/1‑251 (MQ=255)                                                                                                                                                                                                                            gCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGa                                                                    >  2:146152/1‑198 (MQ=255)                                                                                                                                                                                                                            gCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGa                                                                    <  1:146152/198‑1 (MQ=255)                                                                                                                                                                                                                                 cGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATgg          <  1:443726/251‑1 (MQ=255)                                                                                                                                                                                                                                       cATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCGTCATTTGCCAGCGCCCCCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGc                                             <  1:464753/210‑1 (MQ=255)                                                                                                                                                                                                                                       cATCCTTTGTATTGTCTGGATTAATGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGc                                             >  2:464753/1‑210 (MQ=255)                                                                                                                                                                                                                                          ccTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGc  <  1:438508/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             TGGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATT‑AAGTTTGTTCAGAAACGATCCGGGATACACTGCAA‑CATTAAAGCATGACCAGCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGC  >  NC_000913/246503‑246983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑