Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,085,319	G→A	A69A (GCC→GCT)	tsuA ←	thiosulfate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,085,319	0	G	A	100.0%	92.3 / NA	30	A69A (GCC→GCT)	tsuA	thiosulfate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/17);  total (13/17)

AATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCA‑TGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCGGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATG  >  NC_000913/2085091‑2085547                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     aaTAGAGTTATGTTCAGTACTGTAGTGCTGCAAGTTTTGATTTAAACCACTGGCA‑TGTGGAGAACGCTTCCCCGCACCCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCg                                                                                                                                                                                                                <  1:1195/251‑1 (MQ=255)   tagagcaaTGTTCACTCCTGAAGTGCTGAAAGGTTTGATTTAAACCACTGGCC‑TGTGGCGAACGCATCACCGCACTCATCAACATATAAGTGAAAAGCGCGATCCAACTGCCGATCACTCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAACTCAGTAGGCCCGcc                                                                                                                                                                                                              >  2:435835/8‑251 (MQ=255)                 gTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCA‑TGTGGAGCACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCaa                                                                                                                                                                                                 >  1:47955/1‑250 (MQ=255)                   aCTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCA‑TGTGGAGAACGCATCCCCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGACCGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGATTTAACGCaaaa                                                                                                                                                                                               >  1:55670/1‑250 (MQ=255)                            ctgcAAGGTTTGATTTAAACCACTGGCA‑TGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGGGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACACACCTATAACAGTACCACGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCAGCCTGACTTAACGCAAAAACCCCCACGc                                                                                                                                                                                     >  1:262586/1‑251 (MQ=255)                                     ttcaTTTAAACCACTG‑CAGTGGGGAGAACCCATCCCCCCATCCATCACAATATAAGTGAAAAGCGCGACCCAACGGCCGATCACCCCCTCCCCCGCGCGATACCAGGTCCCGGTGCCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTAGAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTAcc                                                                                                                                                                             <  2:348204/247‑1 (MQ=255)                                                               gaacgcatccccgcttccacccccATATATGTGAAAAGCGCTATCCAACGGCGGTCCGTTCCCTCACCCGCTCGATACAAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCCCCTATAACAGTACCTAGCCACGGGAACGCCCCATCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACACAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTaaaa                                                                                                                                                   <  1:193839/229‑1 (MQ=255)                                                                   gCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGACGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATg                                                                                                                                               >  1:18270/1‑251 (MQ=255)                                                                   gCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATCACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTCGGCCCGCCTGACTTCACACAAAAACCCCCACGCTTTGTACCGACATTGCACTCAGCAAGGCGTAAAACAt                                                                                                                                                >  1:313850/1‑250 (MQ=255)                                                                    cATCACCGCACTCATCACCATATAAGTGAACAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGc                                                                                                                                              >  1:407094/1‑251 (MQ=255)                                                                              ataatcaCCTTATAAGTAAAAAGCGCGATCCAACGTCCGTTAAACCCCTCACCAGCGCGATACCAGGTCCCGGTGCCCCATCCTCCCGCCAGAACAATTCCCAGCCCTAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATTCGATTAttttt                                                                                                                                    <  1:320473/248‑1 (MQ=255)                                                                                catccCCATATAAGGAAAAAGCGCGATCCAACGGCCGAACACTCCCCCCCCCGCCCGATACCGGGTCCCGGTGGCACATCCACCCGCCAGAACATTTCCCATCCTGAAGATAGCCCCACCTAGAACAGTACCAAGCCACGTGAACGCCTCAGCTTCGTAAGTCAGTAGGCCCGCCTTAATTAACGCATAAACCCCCGCGCTTTGTAGCGAAATTGCAATCAGCAAGGCGTAAGACagtctttgattgtt                                                                                                                                    <  2:217444/249‑14 (MQ=255)                                                                                 acccctatatAAGTGAAAAGCGCGACCAAACTGCCGATAACACCACCACCCGCGCCAAACCAGGACCCGGTGCCACATCCTCCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCGTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAATGCGTAAAACATGCGATTATTTTTCAc                                                                                                                                 <  2:206841/245‑1 (MQ=255)                                                                                        tataAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATAc                                                                                                                          >  2:383581/1‑251 (MQ=255)                                                                                                   aGCGCAATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGAAAGTCAGTAGGCCCGCCTGAATTAACGCAAACACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGACGTAAAACATGCGATTCTTTTTCACGATATACATATCGCGac                                                                                                                >  2:328817/1‑249 (MQ=255)                                                                                                         aTCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAaccacc                                                                                                         >  2:254869/1‑251 (MQ=255)                                                                                                            cAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGt                                                                                                      >  1:437738/1‑251 (MQ=255)                                                                                                                             cccccccgcccgATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATCCCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCAc                                                                                     <  1:383581/247‑1 (MQ=255)                                                                                                                               cccccGCGCTCTACCAGGTCCCGGTGCCACCTCCCCCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAATTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCAc                                                                                     <  2:407094/247‑1 (MQ=255)                                                                                                                                       cgATACCAGGTCCCGGTGGCACCTCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCg                                                                                                                                                                                                                >  2:420880/1‑118 (MQ=255)                                                                                                                                       cgATACCAGGTCCCGGTGGCACACCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCg                                                                                                                                                                                                                <  1:420880/118‑1 (MQ=255)                                                                                                                                        gatacatagccccgttggccccaccACCCTCCAGAAAAATTCCCAGCCGTAAGATATACCCACCTATACCAGGACCAAGCCCAGGAAAATCCCCAGCGTCGTAAGTCAGTAGGGCCGCATGAATTAACGCAAAAACCCCCAAGCTTTGGACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATAATTTTTCACGATATACATAGCGCGAAAACCACCTGTCAGGCAAAAACTCCCACGCTGCATGACa                                                                          <  2:215369/228‑1 (MQ=255)                                                                                                                                         aTACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACAAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCttt                                                                                                                                                                                  >  1:42564/1‑146 (MQ=255)                                                                                                                                                       ggcccaatccctccaccAGAACAATCCCCAGCCCTAAGATTGTCCCGCCTATACCAGTCCCAAGCCCCGGGAACGCCCCAGCTTCGTAAGATAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCACTCATCAAGGCGTAAAACATGCGATTATTTGTCGCGATATACATATCGCTAAAACCACCTGTCAGGCAAAAACGCCTACGCTGCAGGACAAATccctggatagca                                                           <  2:172699/236‑10 (MQ=255)                                                                                                                                                       cggcccaTCCCCCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGca                                                           <  2:174508/246‑1 (MQ=255)                                                                                                                                                                           aCAATTCCCAGCCAGAAGAGAGCCCCACCTATAACAGTACCAAGCAACGGGAACGCCCCAGCTGCGTAAGTCAGTAGGCCCGCCTGAATGAACGCAAAAACCCCCACGCTGTGTACCGAAATTGAAATCAGCAAGGAGTAAAACATGCGATGATTTGTCACGATATACAGAGCGAGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCTCGCtgaa                                       <  2:313850/251‑4 (MQ=255)                                                                                                                                                                                   cAGCCCGAAGATATACCCACCTAGACCAGTACCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGTCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCGCGCTGCATGATAAAGCCCAGCAGATCACCACAAATTAGCCCGCTTAATATCATTg                               <  2:437738/251‑1 (MQ=255)                                                                                                                                                                                         gaagacaaaCCCACCTAAAACACAACCAAGCCACTGGAACGCCCCAGCTTCGTAAGGCAGTAGGCCCGCCAGAATTAACGCAAAAAGCCCCACTCTTTGGACCGAAATTGCAATCAGCAAGGCGTATAACACGCGATTAATTTTCACGATATACATATCTCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTTGCCCGCTTAATATCATTGAAAACa                         <  2:383150/243‑1 (MQ=255)                                                                                                                                                                                                                 aCCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACTCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATg  <  2:20315/250‑1 (MQ=255)                                                                                                                                                                                                                 aCCAAGCCACGGGAACGCCCCAGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACTAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACAGAAATTAATTAACCAGATGAATg  <  2:47955/250‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     AATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCA‑TGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTGGCACATCCACCCGCCAGAACAATTCCCAGCCCGAAGATATACCCACCTATAACAGTACCAAGCCACGGGAACGCCCCGGCTTCGTAAGTCAGTAGGCCCGCCTGAATTAACGCAAAAACCCCCACGCTTTGTACCGAAATTGCAATCAGCAAGGCGTAAAACATGCGATTATTTTTCACGATATACATATCGCGAAAACCACCTGTCAGGCAAAAACGCCCACGCTGCATGACAAATCCCAGCAGAGCACCACAAATTAGCCCGCTTAATATCATTGAAAACATAAATTAATTAACCAGATGAATG  >  NC_000913/2085091‑2085547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑