Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,046,506	G→T	V523V (GTG→GTT)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,046,506	0	G	T	100.0%	74.9 / NA	23	V523V (GTG→GTT)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/9);  total (14/9)

GAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCG  >  NC_000913/2046260‑2046743                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              gAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCg                                                                                                                                                                                                                                           <  1:248820/251‑1 (MQ=255)         gagaAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAAATAGTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCGGGCCGAGGGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCa                                                                                                                                                                                                                                   <  1:193762/251‑1 (MQ=255)            aaGTGAAATCACTGGTTTGGTCGCTACAAACAAAATATGCCCTGAAAGGATATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGGGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACAGATAAAACCTGGCCGATTGAAGTCACCGCAGAAGATGTCAAAGACAATTTGTCGAATCGTGACCAGAGCTTGGTTGAAGTTCAGGcccc                                                                                                                                                                                                                                 >  2:259312/1‑247 (MQ=255)             aGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTa                                                                                                                                                                                                                               <  1:433302/251‑1 (MQ=255)                             tcgtcgCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGaa                                                                                                                                                                                                                  >  1:263200/1‑248 (MQ=255)                                                             cTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAACCATTg                                                                                                                                                                                >  1:262053/1‑250 (MQ=255)                                                              tataACGTAGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGACACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGACCCCACACATTGa                                                                                                                                                                               >  2:193260/1‑250 (MQ=255)                                                               ataACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGa                                                                                                                                                                               <  1:158994/249‑1 (MQ=255)                                                                    gTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCgg                                                                                                                                                                         >  1:353819/1‑250 (MQ=255)                                                                           ccACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATGCCCAt                                                                                                                                                                 <  2:432126/251‑1 (MQ=255)                                                                                gCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACCTTGAACGCGGATTCCCATTCAAc                                                                                                                                                            >  1:167279/1‑251 (MQ=255)                                                                                 cACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAAcc                                                                                                                                                           <  1:290539/251‑1 (MQ=255)                                                                                               ggTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGCAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGcc                                                                                                                                                                                                                      >  2:234185/1‑178 (MQ=255)                                                                                               ggTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGGCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGcc                                                                                                                                                                                                                      <  1:234185/178‑1 (MQ=255)                                                                                                                                   gttacgctgccaactaaCCGGTTCACCAGTACGCCAGCAACCGATAACACCTCGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGATCAGCCAAAAAGATTCCTCGGTATCGTTCAGTACCCAAAAATTGAACGCGGATTCCCATTCAACCGCCACACTCACTTTTATTGCACATGATGCAGCAGGTAATCCtgttgcagg                                                                                                         >  2:264691/17‑247 (MQ=255)                                                                                                                                                                                   cccTGGCCGTTTGAAGTCCCCGCCGAAGATGTCAAAGGAAATTTGTCGAATCGTAAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAAAAAGATTCCTCGGTATCGTTAAGTACCAAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTc                                                         <  1:351003/250‑1 (MQ=255)                                                                                                                                                                                     cacgccgttcttccgatcgtccgAAGATGGAAAGGGCATTTTGTCAAATCGGAAACAGAGCTTGGTTGTGTTTCAGGCCCCTACGCAAAGCCAGAAATATCCCGCGTTATCGTAAAGAACCCAAACATTGAACGCGGATTCCCATTCACCCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCTCCCTTTCTg                                                       <  2:387199/231‑1 (MQ=255)                                                                                                                                                                                           gATTGCAGTCACCGCCGAAGATGTCAAAGGCAATTTGTAGACTCGTGAACAGCGCATGGTTGTCGTTCAGGCACCTACGCTAAGCAAGAAAGATTCCTCGGAATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGGGCTCTCGACGAGTCACGAAGGCGTTCAGGACATCACCCTTTCTGCCTgg                                                  >  2:409934/1‑250 (MQ=255)                                                                                                                                                                                                     aCCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATgg                                       >  1:263082/1‑251 (MQ=255)                                                                                                                                                                                                       cgccgAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAA‑CATTGAACGCGGATTCACATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGAATGGAAAGATAATGGTgc                                    >  1:62041/1‑250 (MQ=255)                                                                                                                                                                                                        gccgAAGATGTAAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTg                                                       >  1:387199/1‑232 (MQ=255)                                                                                                                                                                                                                       ggCAATTTGTCGAATCGTGAACAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATAccc                      >  1:222355/1‑250 (MQ=255)                                                                                                                                                                                                                                           aCAGAGCATGGTTGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCg  >  2:206761/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              GAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGTAAAGATATTCTGGTTACCCTGCCGGCTTACCGGTTCACCAGTACGCCAGAAACCGATAACACCTGGCCGATTGAAGTCACCGCCGAAGATGTCAAAGGCAATTTGTCGAATCGTGAACAGAGCATGGTGGTCGTTCAGGCACCTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCG  >  NC_000913/2046260‑2046743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑