Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,041,411	G→A	G13S (GGT→AGT)	zinT →	metal‑binding protein ZinT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,041,411	0	G	A	100.0%	59.2 / NA	18	G13S (GGT→AGT)	zinT	metal‑binding protein ZinT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/8);  total (10/8)

ATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAAC  >  NC_000913/2041175‑2041634                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                 aaccGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTAAAAGCACCAAAATAATTTAATTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAGCTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGGTGCTTTAAGTGTCTTTATT‑g                                                                                                                                                                                                                     <  2:57261/247‑1 (MQ=255)                                                    aaaTCACCAAAATAATTTATTTTATGTCAATAAGAAAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACAGTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAGCCCTTAACa                                                                                                                                                                <  2:369548/251‑1 (MQ=255)                                                                              tCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTTGTTAGCGCTCCTGCCTTTTCGCATGGTCAtcactca                                                                                                                                                                                  <  1:272999/208‑1 (MQ=255)                                                                              tCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATTTGTTAGCGCTCCTGCCTTTTCGCATGGTCAtcactca                                                                                                                                                                                  >  2:272999/1‑208 (MQ=255)                                                                               cAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATggg                                                                                                                                     >  2:131391/1‑250 (MQ=255)                                                                                  tAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAACGATGCTAATGGTGtt                                                                                                                                  >  2:386187/1‑251 (MQ=255)                                                                                   aaGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAAc                                                                                                                                                      <  1:323639/230‑1 (MQ=255)                                                                                   aaGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAAc                                                                                                                                                      >  2:323639/1‑230 (MQ=255)                                                                                        atataAGTATAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATAGAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCGTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGGCTTTTCGCATTGTCAGCACTCACACGTCAAACCCTTAACAGAGTTCGAACAAAAAGCTGCTAATGGTGTTTTtgat                                                                                                                            <  2:273836/249‑1 (MQ=255)                                                                                                                                          aTTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCAGCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCa                                                                          <  2:338900/251‑1 (MQ=255)                                                                                                                                                             tataACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTAGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTg                                                       >  2:242441/1‑251 (MQ=255)                                                                                                                                                               taACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGaa                                                                                                      <  1:38428/202‑1 (MQ=255)                                                                                                                                                               taACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGaa                                                                                                      >  2:38428/1‑202 (MQ=255)                                                                                                                                                                                               ggaAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACACTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGAGGCAAACTTGACCCCGTCTTTCagc                        >  1:244871/1‑247 (MQ=255)                                                                                                                                                                                                          tGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGc           >  1:42730/1‑250 (MQ=255)                                                                                                                                                                                                              gATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGAt       >  1:251262/1‑250 (MQ=255)                                                                                                                                                                                                              gATTCGTCTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGAt       >  1:38376/1‑250 (MQ=255)                                                                                                                                                                                                                  cggcTTTACAAACTGGCTGTTGCTTTAAGTGTCTTTATG‑GTTAGCGCTCCTGCCTTTTCTCATGGGCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGAtaaaac  <  2:244871/248‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                 ATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAAAGCCATGCTCTCGTTTCCTAAGAGTTGTTGCATTTTGCTATATGTTACAATATAACATTACACATCATATACATTAACTCTGGAGGAAACTGTTTTGGCGATTCGTCTTTACAAACTGGCTGTTGCTTTAGGTGTCTTTATT‑GTTAGCGCTCCTGCCTTTTCGCATGGTCATCACTCACACGGCAAACCCTTAACAGAGGTCGAACAAAAAGCTGCTAATGGTGTTTTTGATGATGCCAATGTACAAAACCGAACGCTCAGTGACTGGGATGGAGTCTGGCAATCCGTTTATCCTTTACTGCAAAGTGGCAAACTTGACCCCGTCTTTCAGAAGAAAGCGGATGCAGATAAAAC  >  NC_000913/2041175‑2041634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑