Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,776,100 G→A E172K (GAA→AAA)  ydiF → putative acetate‑CoA transferase YdiF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,776,1000GA95.5% 72.4 / NA 22E172K (GAA→AAA) ydiFputative acetate‑CoA transferase YdiF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (11/10);  minor base C (1/0);  total (12/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTG  >  NC_000913/1775860‑1776334
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          
ttATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCATCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGACGAGTTTGATAACAAAAAatagttcgg                                                                                                                                                                                                                                  >  2:87423/1‑245 (MQ=255)
ttATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAat                                                                                                                                                                                                                                         <  1:87423/244‑1 (MQ=255)
      ggTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACaa                                                                                                                                                                                                                            <  2:312275/251‑1 (MQ=255)
             ggggACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGAtt                                                                                                                                                                                                                     <  2:89462/251‑1 (MQ=255)
                       gccgcGTATTTCTGCACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCCCGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGACGAGTTTGATAACAACAAATATCTCTATTACAAAGCGATTGCGCCAGat                                                                                                                                                                                                            >  1:108387/1‑250 (MQ=255)
                                            aGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTacc                                                                                                                                                                                       >  1:136203/1‑250 (MQ=255)
                                                                    taACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTCGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATACCAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGccc                                                                                                                                                              >  2:457700/1‑250 (MQ=255)
                                                                               aaGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGa                                                                                                                                                       >  2:20574/1‑247 (MQ=255)
                                                                                aGGTGAACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTATATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCAACAGTGAAGGCTACGCCACTTTTGAAGATg                                                                                                                                                  >  2:375539/1‑251 (MQ=255)
                                                                                               aaCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTCATATTGGCATCGGGACATTTGTCGATCCACGCCAGCATGGCGGCAAACTGAATGAAGTCACTAAAGACGACCTGATTAAACTGGTCGAGTTTGATAACAAACAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCg                                                                                                                                   >  2:151131/1‑251 (MQ=255)
                                                                                                 ccTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGAc                                                                                                                                 <  1:25983/251‑1 (MQ=255)
                                                                                                     aCGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTCCCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCAt                                                                                                                             >  2:118628/1‑251 (MQ=255)
                                                                                                      cgcgCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATGGCCTTCATTCGCGCTACCACCTGCGACAGTGAGGGCTACGCCACTTTGGAAGATGAGGTGATGTATCTCGACGCAtt                                                                                                                            <  1:151131/251‑1 (MQ=255)
                                                                                                                                   tattaGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAAc                                                                                                <  1:20574/250‑1 (MQ=255)
                                                                                                                                   tattaGTGATATTCGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCCATTGCGCCAGATATTGCCTTCATTCGCGCTTCCACCTGCGACAGTGAAGGCTACGCGACTTTTGAAGATGAGATGATGTATCTCGACGCATTGGTTTTTGACCAGGCGGTGCACAATAAc                                                                                                <  2:331434/250‑1 (MQ=255)
                                                                                                                                                    tCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAACCTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGatgatg                                                                              >  1:186820/1‑251 (MQ=255)
                                                                                                                                                                       aCGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTTCCTCCTGCGACAGTTAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCTGTATTGTGATGATGCTGGTGCAGAAAATGGTTa                                                           <  1:337710/251‑1 (MQ=255)
                                                                                                                                                                          ccAGCAAGGCGGCACACTGAATGCAGTCACTAAAGACGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGCTTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAg                                                                                                               >  2:415016/1‑196 (MQ=255)
                                                                                                                                                                          ccAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAAGATCTCTATGACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAg                                                                                                               <  1:415016/196‑1 (MQ=255)
                                                                                                                                                                                                   gTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGGTGATTCAGGTGGCGAAAAAGGTTaaaaaaagcaccacgcatccctaatctgt                               >  2:109596/1‑228 (MQ=255)
                                                                                                                                                                                                                    gATTAAACTGGTCGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATc              <  1:165412/251‑1 (MQ=255)
                                                                                                                                                                                                                                cGAGTTTGATAACAAAAAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGATGATGCAGGTGCAGAAAATGGTTAAAAAAAACACGCTGCATCCTAAATCTTTCCGTATTCCCGGttttttgggggggattt  >  1:219522/1‑236 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          
TTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGGTCGAGTTTGATAACAAAGAATATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTG  >  NC_000913/1775860‑1776334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: