Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 88,571 G→A V182M (GTG→ATG)  cra → DNA‑binding transcriptional dual regulator Cra

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091388,5710GA98.3% 183.8 / NA 60V182M (GTG→ATG) craDNA‑binding transcriptional dual regulator Cra
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (27/30);  minor base C (1/0);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACA‑GCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGCGACGGCA  >  NC_000913/88327‑88814
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
agaTCAGCCAGACAACGAAATGCGGTGCATTTATCACTTTTTACA‑GCGTCAGGTTGATGCCATTATTGTTTCGCCGTCTTTCCCCCCTGAGCATCCTTTTTATCACCGCGGGGAT‑AACGACCCTTTCCCGATTGTCGCGCTGGCCCGCGCCCTCGATCGTGAACAC‑TTCCCCAGGGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGGGTTACGTAAGTTTCCCGCCGAGACGATGCttt                                                                                                                                                                                                                                               <  2:325022/251‑1 (MQ=255)
  aTCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACA‑GCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTAt                                                                                                                                                                                                                                             >  2:67617/1‑251 (MQ=255)
               cgacaTGCGGTGCATTGAGCACCTTTTACA‑GCGTCAGGTTGATGCAATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGGTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTAc                                                                                                                                                                                                                                >  2:303221/5‑251 (MQ=255)
               cGAAATGCGGTGCATTGAGCACCTTTTACA‑GCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGCTCAGGCTGATGCCGAAATGCAGGCGGAAGAGTTACGTAAGTTTCACGCCGAGACGCTGCTTTATCTTGGTGCGCTAc                                                                                                                                                                                                                                >  2:303209/1‑251 (MQ=255)
                gaaattcggtgaatggacccccTTTTCCA‑GCGACAGGTAGATGCCATTATTGTTCCGACGTCGTTGCCTCCTGATCATCCTTTTTAGCAACCCTGGGCT‑AACGCCCCGTCCCCGATTGTCGCTCTGGACCGCGCCTTCGACCGTAAACGT‑TTCACCAGAGTGGTTGGTGCCGACCAGGATGATGCCGAAATGCTGGCGGAAGAGTTCCGGAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCttcc                                                                                                                                                                                                                               <  2:146985/231‑4 (MQ=255)
                   agtgggtactgtgggCACCTTTTACG‑GGGTCAGGTTGATGCCTTTATTGTTTCGCCGTCGTTGCCCCCTGAGCTTCCTTTTTATCAACGCTGGGCT‑AACGTCCCTTTCCCGTTTGTCGCTCGGGACCGCGCCCTCGATCGGGAACCC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGCTTTACGTAATTTCCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGa                                                                                                                                                                                                                            <  2:353658/237‑1 (MQ=255)
                          gCATTGAGCACCTTTTACA‑GCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCt                                                                                                                                                                                                                     >  1:402857/1‑251 (MQ=255)
                               gAGCACCTTTT‑CACGCGTCAGTTTGATGCAATTATTGTTCCGTCGTCGTGGCCCCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGCCCCGTTCCCGATTGTCGCGCTGGCCCGCGCCCTCGACCGTGAACAC‑TTCCCCGGCGTGGTTGGTGCCGATCAGGAGGATGCCGAAAGGCTGGCGGAAGAGTTACGAAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAg                                                                                                                                                                                                                <  2:356098/251‑1 (MQ=255)
                                aGCACCTTTTACA‑GCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGc                                                                                                                                                                                                               >  1:162839/1‑251 (MQ=255)
                                aGCACCTTTTACA‑GCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGGTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGGTGCTTTATCTTGGGGCGCTACCGGAGCTTTCTGTCAgg                                                                                                                                                                                                               >  2:77691/1‑250 (MQ=255)
                                  cACCTTTTACA‑GCGTCAGTTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTGTTAACAACGCGGGGCA‑AACGCCCCGTCCCCGATGGGCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGTTGGGTGCCGAGCAGGATGAGGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTCCCCGCCGATACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCtt                                                                                                                                                                                                             <  1:137586/251‑1 (MQ=255)
                                        ttACA‑GCGTCGGGTTGATGCCTTTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGAGGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGc                                                                                                                                                                                                        <  1:58376/250‑1 (MQ=255)
                                                     gTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGttt                                                                                                                                                                                           >  1:359770/1‑251 (MQ=255)
                                                       tGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGATCTTCCTTTTTACCACCGCTGGGAA‑AACGACCCGTTCCCGATTGTCGCGTTGGACGGCGCCTGCGATCGTGAACAC‑TTCCCCAGCGTGGTTGGTGCCGATCAGGATGTTGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTCCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGtgtc                                                                                                                                                                                          <  2:355786/250‑4 (MQ=255)
                                                        taTGCCATTATTTGTTCAACGTTTTTGCCCCCTGTGCATCCTTTTTATCACCGCTGGGCT‑CACGACCCGTTCCCGTTTGTCGCGCGGGACCGCGCCCTCGTTCGTGAACTC‑TTCACCAGCGTGGTTGGTTCCGATCAGGATGATGCCAAAATTCTGGCGAAAGAGTTACGAATGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTcc                                                                                                                                                                                         <  2:449656/249‑1 (MQ=255)
                                                         aTGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGCCCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGt                                                                                                                                                                                       >  2:23526/1‑251 (MQ=255)
                                                                tattTTTTCGACGGCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGcc                                                                                                                                                                                 <  1:404993/250‑1 (MQ=255)
                                                                tattGTGTCGACGGCTTTGCCTCCTGAGCATCCTTTTTATCACCG‑TGGGCTTAACGACCCTTTCCCGATGGTCGCGCTGGACCGCGCCCTCGACCGTGAACTC‑TCCCCCAGCGTGGTTGGTGCCGACCAGGATGATGGCGAAATGGTGGCGGAAGAGTTCCGTAATTTTCCCGCCGAGACGATGCTTTAGCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGcc                                                                                                                                                                                 <  2:230980/250‑1 (MQ=255)
                                                                                                               gggCT‑AACGACCCGTTACCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGCGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGACGGGCATTTCCTGTATGCCAACAGCTAt                                                                                                                                 >  2:281867/1‑251 (MQ=255)
                                                                                                               gggCT‑AACCCCCCGTCCCCGGTTGTCGCGCTGGCCCGCGCCCGCGATCGTGA‑CACCTCCCCCAGCGTGGTGGGTGCCGATAAGGATGATGCCGAAATGTTGGCGGAAGAGTTACGTAATTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTAt                                                                                                                                 <  2:408410/251‑1 (MQ=255)
                                                                                                                 gCT‑AACGACCCGTTCCCTATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCCCCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGa                                                                                                                               <  1:286308/251‑1 (MQ=255)
                                                                                                                                   gATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGAGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGATTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATACGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGccc                                                                                                              >  1:296318/1‑251 (MQ=255)
                                                                                                                                     ttGTCGCGCTGTACCGGGCCCTCGATCGTGAACAT‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACTTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCa                                                                                                             <  1:219329/250‑1 (MQ=255)
                                                                                                                                      tGTCGCGCTGGACCGCGCCCTCGAGCGTGACCAC‑TTCCCCAGCGTGGGTGGTTCCGATCAGGAGGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAg                                                                                                            <  2:388799/250‑1 (MQ=255)
                                                                                                                                        tCGCGCTGGACCGCGCCCTCAATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAACTGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAACGATGATCCGAGCGGAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGtt                                                                                                          >  2:290127/1‑250 (MQ=255)
                                                                                                                                           cgcTGGACCGCGCCCTCGATCGTGAACAC‑TTCAACAGCGTGGTTGGTGCCGATCAGGATGATGCCGAACTGCTGGCGGAAGCGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGttt                                                                                                                                                                                           >  2:26985/1‑166 (MQ=255)
                                                                                                                                                   cgcgCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATg                                                                                              >  1:375220/1‑251 (MQ=255)
                                                                                                                                                          tCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGa                                                                                        >  2:361004/1‑250 (MQ=255)
                                                                                                                                                           cgttcggaaaCAC‑TTCACAGGCGTGTTTGGTGCAGATCAGGATGATGCCGAAATGTGGGCGGAAGAGTTCCGAAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGa                                                                                        <  2:252677/241‑1 (MQ=255)
                                                                                                                                                           cGATCGTGAACAC‑TTCACCAGCGTGGTTGTTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGaaa                                                                                      <  1:216514/251‑1 (MQ=255)
                                                                                                                                                                gTGAACAC‑TTCACCAGTGTGGTGGGGGCCGATCAGGAGGAGGCCGAAATGCTGGGGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCAt                                                                                 <  2:162839/251‑1 (MQ=255)
                                                                                                                                                                gTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGCTGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGGGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTAATCGAAAAATGGCTGGAAACGCAt                                                                                 >  2:13033/1‑251 (MQ=255)
                                                                                                                                                                    accc‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGATTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGa                                                                             <  1:358145/248‑1 (MQ=255)
                                                                                                                                                                    acac‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCg                                                                              >  1:335529/1‑250 (MQ=255)
                                                                                                                                                                    acac‑TTCACAAGCGTGGTTGGTGCCGATCAGGATGCTGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCg                                                                              >  1:129765/1‑250 (MQ=255)
                                                                                                                                                                          tcaccaaggtgggtggTGCGGAGCGGGATGATGCCGAAAGGCTGGCGGAAGAGTTACTTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGcc                                                                         <  1:67617/242‑1 (MQ=255)
                                                                                                                                                                               aGCGTGGTTGGGGCCGATCAGGAGGATCCCTAAATGGTGGCGGAAGATTTACTAAATTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTGCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCACCAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGgc                                                                   <  2:262237/251‑1 (MQ=255)
                                                                                                                                                                                     gttggtGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCCGGCGCTGtt                                                             >  2:101842/1‑251 (MQ=255)
                                                                                                                                                                                        ggtGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGCAACGCATCCGATGCCGCAGGCGCTGTTcac                                                          >  2:240460/1‑251 (MQ=255)
                                                                                                                                                                                              gATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGGACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGCAAAATGGCTGGAAACGCAACCGATGCCGCAGGCGCTGTTCACAAcgtc                                                    >  2:351688/1‑251 (MQ=255)
                                                                                                                                                                                                tCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAAcgtcgt                                                  >  1:105629/1‑251 (MQ=255)
                                                                                                                                                                                                 cAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGTCGCTGTTCACAACGTCGtt                                                 >  1:466022/1‑251 (MQ=255)
                                                                                                                                                                                                  tggATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGCGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGttt                                                >  2:21052/2‑251 (MQ=255)
                                                                                                                                                                                                    gatgagGCCGAAATTCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGc                                              <  1:314515/251‑1 (MQ=255)
                                                                                                                                                                                                          gCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCgttgtt                                        >  1:9752/1‑251 (MQ=255)
                                                                                                                                                                                                          gCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCgttgt                                         <  1:101842/250‑1 (MQ=255)
                                                                                                                                                                                                               aaTGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAg                                   <  1:235482/251‑1 (MQ=255)
                                                                                                                                                                                                                aTGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAgg                                  >  1:29802/1‑251 (MQ=255)
                                                                                                                                                                                                                        ggAAGTGTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTgatgg                          <  1:296347/251‑1 (MQ=255)
                                                                                                                                                                                                                        gaaagtgttatggaatttccccGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTTCGTGAACAAGGGTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCAGTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTgatgg                          <  2:385263/232‑1 (MQ=255)
                                                                                                                                                                                                                         gaagaaTTACGAACGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTgatggg                         >  2:188736/1‑250 (MQ=255)
                                                                                                                                                                                                                              gtttggTAATTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCa                    <  1:351688/246‑1 (MQ=255)
                                                                                                                                                                                                                              gTTACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCa                    <  1:64521/251‑1 (MQ=255)
                                                                                                                                                                                                                               ttACGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCAc                   >  1:261377/1‑251 (MQ=255)
                                                                                                                                                                                                                                 ccttAAGTTTCCCGCCGACCCGTTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGGGAACAAGGGTTCCGTACGGCCCGGAAAGATGATCCGCGCGAATTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGGCCAGTTAATCTAAAAATGGTTGTAATCGCAACCGATGCCGCAGGCGCTGTTCACAACTGCGCTTGCCTTGTTGCAAGTAGTGATGGTCGTCACg                  <  2:45065/247‑1 (MQ=255)
                                                                                                                                                                                                                                  cGTAAGTTTCCCGCCGAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCt                <  1:281867/251‑1 (MQ=255)
                                                                                                                                                                                                                                            ccgccgAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGCGAc      <  2:335529/251‑1 (MQ=255)
                                                                                                                                                                                                                                             cgccgAGACGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAATATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGCGACg     <  1:87030/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 gagaCGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGCGACGGCa  >  1:336066/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 gagaCGATGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCATGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAGGGAGCGATGGATGTCAAGCTGCGTCGCGACGGCa  >  2:101696/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
AGATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACA‑GCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCT‑AACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACAC‑TTCACCAGCGTGGTTGGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAGGCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGCGACGGCA  >  NC_000913/88327‑88814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑