Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	46.8 / NA	13	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/8);  total (5/8)

TCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATG‑ACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCC  >  NC_000913/1652114‑1652571                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 tCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAAGACATAGATCTGAAAAATGGAACAATGATGATCAAGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAGCCAGCAATCGACTCTCTCCGGAGTCTGATG‑ACATTAACGATACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAcacaccat                                                                                                                                                                                                                  <  2:460572/250‑5 (MQ=255)     ccTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAAACGGG‑CAATTATCTTATTAAGCCAGCAAACGACGCTCTCCCGGGTCCGATGGAC‑TTAACGCGAATTAGAAAAAAGCATAACATTGTCGGTCACCTCAGATAGAATGGAAGACCAGAAAAAAAAAAATCCAc                                                                                                                                                                                                             >  2:255798/1‑251 (MQ=255)        ggTGAGCTGTGTGCACGTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATG‑ACATTAACGAGACTGAGCAAAGAGCATATAATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCtt                                                                                                                                                                                                          <  2:16898/251‑1 (MQ=255)                                                            tgatgaTCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGGCATTCATCTTATTAAGCCAACAAAAGCCGCTCTCCGGAGGCAGATT‑ACATTAAAGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCCGAGAGTATGGCAGAACAGAAAAAAAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGGAAAAAAATATGGTGAACAtttt                                                                                                                                                      >  2:104632/1‑251 (MQ=255)                                                               tgatCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGGCATTCATCTTATTAAGCCAGCACTCGACGCTCCCCCGCGTCAGATG‑CCATTTACGAGACTGAGCAAAGAGCCTATCATTGAGGTTCACCTCAGAGAGAATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAAACCGAAGTGTCAGCGAGAGGAAAAAATTAttgggtacattttacc                                                                                                                                                   >  2:453033/1‑236 (MQ=255)                                                                      gagaAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATG‑ACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGTTGACCATTTTACCGTTGACt                                                                                                                                            <  1:2063/251‑1 (MQ=255)                                                                             ttAGCAAAAGACCGTTTCACGGGACCAAAAACCCAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAGCCAGCAAGCGACGCTCTCCGGAGTCAGATG‑CCATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCTCCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTGCCGTTGACTtaataa                                                                                                                                      <  2:152295/250‑1 (MQ=255)                                                                                                                          aaT‑CGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATG‑ACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCg                                                                                        <  1:61057/251‑1 (MQ=255)                                                                                                                               gggTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATG‑ACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGaaaa                                                                                    <  1:267439/251‑1 (MQ=255)                                                                                                                                  tcattaatCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATG‑ACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATc                                                                                  >  2:196644/1‑250 (MQ=255)                                                                                                                                                                                                               cATTGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATg      <  1:196644/250‑1 (MQ=255)                                                                                                                                                                                                                  tGATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAAGCATATCAGTCGAGACATTCTTATGCCTGCGGGTCGCTGACAGCTGGTGCTAACCCTGCATTTATACCAAACCAGATGGGc   <  2:291850/250‑1 (MQ=255)                                                                                                                                                                                                                   gATGTTCACCTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGcc  >  2:273889/1‑250 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 TCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAAT‑CGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATG‑ACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCC  >  NC_000913/1652114‑1652571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑