Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	59,968	C→T	V127I (GTC→ATC)	rluA ←	23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	59,968	0	C	T	100.0%	104.4 / NA	33	V127I (GTC→ATC)	rluA	23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/16);  total (17/16)

GCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTC  >  NC_000913/59730‑60206                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               gCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCgg                                                                                                                                                                                                                                    >  1:233414/1‑251 (MQ=255)  ccGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTTGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCATCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTGGCCGTGTTATCCGCCGCATACTCCACCACTTCATATGCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATGTTCTGTTTCggg                                                                                                                                                                                                                                   <  2:321666/251‑1 (MQ=255)     gATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCgg                                                                                                                                                                                                                                <  1:27690/251‑1 (MQ=255)                             cATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGGGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCTCACGCAATTGATGCGAACGCCCGGTAATCGGGTTTAACACCACTCTTGCCGTGTTATCCGCCGCATGCGCCACCACTTCATATTCCGTCTGCGCAGGTTTGCCCGTTTCGGAGCAGATTTTCTGGTGCGGGCTGTTTGGCCATGCGCAAATCAGCGGc                                                                                                                                                                                                        <  2:71910/251‑1 (MQ=255)                                 tAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGc                                                                                                                                                                                                        >  1:460014/1‑247 (MQ=255)                                      acaaACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATAGGCACACGCAATTGATGCGAACGTCCGTTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGTTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATccacc                                                                                                                                                                                               <  1:168017/251‑1 (MQ=255)                                                 gCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTg                                                                                                                                                                                     <  1:373613/250‑1 (MQ=255)                                                            cGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGggatggat                                                                                                                                                                         <  2:233414/251‑1 (MQ=255)                                                            cGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGggatgga                                                                                                                                                                          <  1:101665/250‑1 (MQ=255)                                                                         gCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATCCTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTGCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGTCCTTCTGCGGGGGATTGATGCCCCCAGAcgcg                                                                                                                                                            <  2:102902/251‑1 (MQ=255)                                                                          cATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCg                                                                                                                                                                                                          >  1:147194/1‑204 (MQ=255)                                                                          cATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCg                                                                                                                                                                                                          <  2:147194/204‑1 (MQ=255)                                                                                 aCGATCGCCGGGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGAGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCAACAGACCTTCTGCGGGGGATGGCTGCCCCCAGACGCGGGCCACAt                                                                                                                                                    >  2:371833/1‑251 (MQ=255)                                                                                  cGATCGCCGAGAATCGGATGACACAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGCCTGCGCAGGTTTACCCGATTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGACAGTCGCAAATCAGCGGCAGATCCACCAAACCTTCTGCGGGGGATGGACGCCCCCCGACGCGGGCcacct                                                                                                                                                    >  1:241473/1‑248 (MQ=255)                                                                                   gATCGCCGAGAATCGGATGACCCCGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCcacagac                                                                                                                                                  >  1:117851/1‑248 (MQ=255)                                                                                         cGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCttt                                                                                                                                            <  1:146484/251‑1 (MQ=255)                                                                                                      aCCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcgct                                                                                                                               <  1:19934/251‑1 (MQ=255)                                                                                                                        cacaCGCAATTGATGCGACCGCACGGTCATCGGTTTTAAAACCACTCCTGCCGGGATAGCCACCGCATACTCCACCCCTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCCGCGGCAGATCCACCAGACCTTCTGCGGGGGGTGTATTCCCCAAGACGCGGGCCACATACTGCTTTTTCGGCACGCGCTCGAGGGACTGGCGtttt                                                                                                              >  2:200289/1‑250 (MQ=255)                                                                                                                         acacGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCGCGCGGAACTGGCGTTTTa                                                                                                             >  1:295911/1‑250 (MQ=255)                                                                                                                                 ttGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGGTTTAACTCCCGcc                                                                                                    >  2:81091/1‑250 (MQ=255)                                                                                                                                  tGATGCGAACGCCCGGTACTCGGCTTTAACCCCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGCCGCGGGCCACATCATGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTACCTCCCGCTc                                                                                                   >  2:65956/1‑251 (MQ=255)                                                                                                                                                      cGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGGCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCTCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCTGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGcc                                                                                <  1:43857/250‑1 (MQ=255)                                                                                                                                                          tttAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCATCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCg                                                                                                                                        <  1:429535/190‑1 (MQ=255)                                                                                                                                                          tttAACACCAATCTTGCCGTGTTATCCGCAGCATACTCAACCACTTCATCTTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCg                                                                                                                                        >  2:429535/1‑190 (MQ=255)                                                                                                                                                            taactcctctctTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGGCTGCGCAGGTTTACCCGTTTCGTAACAGATTTGCTGTGGCGGGCGGGTTGGCCAGTCGCAAAGCAGCGGCAGATCCACCAGACCTTCTGCGGGGGAGGGATGCCCCCAGACGCGGGCCACATACTGCTGTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTGCGCTGCTTTGGGCTGCGCCACTACa                                                                          <  2:191243/242‑1 (MQ=255)                                                                                                                                                                  cacTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACACCCGCTCCACGGCTTTCGTCAGCGCCACTACAATCAcgc                                                                   >  2:244533/1‑251 (MQ=255)                                                                                                                                                                           cGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTgg                                                                                                                    >  2:402373/1‑193 (MQ=255)                                                                                                                                                                           cGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTgg                                                                                                                    <  1:402373/193‑1 (MQ=255)                                                                                                                                                                                     gccgcATACTCCACCACTTCATATTCCGTCTGCGCCGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGCTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGATCGTGGAACTGGCGTTTTAACTCCCGATCCCCTGCTTTGGTCCACCCCACTACAATCACGCCGCTCGTAGCCatattc                                                  >  2:156917/1‑247 (MQ=255)                                                                                                                                                                                       cgcATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCCGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGAc                                              >  1:71298/1‑251 (MQ=255)                                                                                                                                                                                                 accacTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGa                                    <  1:231003/251‑1 (MQ=255)                                                                                                                                                                                                                  cTGCGCAGGTTTACACGTTTCCTAACAGATTTTCTGTTTCGGGCGGCTTGGCCAGTCGCAATTCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGACAGCGCCACTACAATCACGCCGCTGGTAGCCATACCCAGACGAGGCCCCGCTTCTGCCTGCggctcat                   >  2:318486/1‑246 (MQ=255)                                                                                                                                                                                                                                    ttCGTAACAGATTTTCTTTTTCGGGCGGTTTGGCCAGTCGCAAATCCGAGGCAGATCCACCAGACCTTCTACGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGCATAATCACGCTGAATGCGCGtc  >  1:402126/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               GCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTCTGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTC  >  NC_000913/59730‑60206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑