Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,357,202	C→T	intergenic (‑92/+221)	sapA ← / ← ymjA	putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,357,202	0	C	T	100.0%	83.4 / NA	24	intergenic (‑92/+221)	sapA/ymjA	putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/14);  total (10/14)

CGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATT  >  NC_000913/1357016‑1357439                                                                                                                                                                                            |                                                                                                                                                                                                                                              cgGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTctct                                                                                                                                                                               >  1:104016/1‑251 (MQ=255)            aTGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCAAGCCATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATAAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTAGCCATCTGTTCAACATTCGTCACCGTGATTTCTCtataactataac                                                                                                                                                                    >  1:43483/1‑251 (MQ=255)                          cAGGCGCGGCGCTTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACtt                                                                                                                                                      >  1:135717/1‑251 (MQ=255)                                       tGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACATAACAACTTAGCGAGGAGCag                                                                                                                                          <  2:442879/250‑1 (MQ=255)                                                   cacaAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGAt                                                                                                                               <  1:275343/249‑1 (MQ=255)                                                      aaGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACTATCGTga                                                                                                                          <  1:345734/251‑1 (MQ=255)                                                             gCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAAccc                                                                                                                   <  1:437032/251‑1 (MQ=255)                                                                           agaCGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTg                                                                                                     <  2:135717/251‑1 (MQ=255)                                                                                       ctgccgcctattatTAAGGGATTTTATGTAAAGCGGCTATCTCACTAATATTTAATGACATTTGCCATTATCGTTTGTG‑TTCAGGCGTCGTAATGAGAGCTGTGTGGCTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCACCATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCCGCAGTGCGTGTc                                                                                          >  2:307476/12‑250 (MQ=255)                                                                                            gcATAATATTAAGGGATTTAATGTAAATAGGCTATCTTACTAATAGTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGGTGAACATGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTAGAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGtt                                                                                    <  2:43034/251‑1 (MQ=255)                                                                                                      aaGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATgc                                                                          >  1:28968/1‑251 (MQ=255)                                                                                                      aaGGGATTTTAAGTAGAGATGCGATCTGACGAAAATTTAATGATATTTGCCATTACC‑TGTGTGGTTCAGGTGTCGTAATGAGGGCTGTGTGGTTTGTCGTTGAACAGGGGACGTCGCCAGCTTTGCAGCATTCGTACCCGTGATTTCTCTATAACTATATGTCACAGATCATGTGGTCGAGGATCAGAGCTGTACGATCGTGAGAAACTCATTAAAGCCCGCTTCATTGCCTGTCATTGttttgcagatg                                                                           <  2:195567/250‑10 (MQ=255)                                                                                                               tATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTa                                                                                   <  1:290045/233‑1 (MQ=255)                                                                                                               tATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTa                                                                                   >  2:290045/1‑233 (MQ=255)                                                                                                               tATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGaa                                                                  <  2:118168/250‑1 (MQ=255)                                                                                                                 tGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTa                                                                                                               >  1:85751/1‑203 (MQ=255)                                                                                                                 tGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTa                                                                                                               <  2:85751/203‑1 (MQ=255)                                                                                                                         ggCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACTCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCg                                                       <  2:272673/251‑1 (MQ=255)                                                                                                                          gCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTCCGATCGTGAGAAACCCATTAACGCACGCTGCAGTGCGTGTCATTGTTATCCAGATGCGGCGTGAACGCTTTTTCCgg                                                      >  2:326060/1‑251 (MQ=255)                                                                                                                             aTCTTACTAATATTTAAGGACATTTGCCATTACCGTTTGGG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTTTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGa                                                     <  2:51407/249‑1 (MQ=255)                                                                                                                                         tttAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGAcc                                       >  1:444429/1‑251 (MQ=255)                                                                                                                                                 cATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCt                               >  1:274498/1‑251 (MQ=255)                                                                                                                                                                        tCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGATCGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAtcattactcatt          <  2:120730/250‑1 (MQ=255)                                                                                                                                                                                cGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCAtt  <  1:31786/250‑1 (MQ=255)                                                                                                                                                                                            |                                                                                                                                                                                                                                              CGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTG‑TTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCATTACCCCATT  >  NC_000913/1357016‑1357439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑