Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,337,3940AG96.3% 71.6 / NA 28S522G (AGC→GGC) acnAaconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base G (10/16);  minor base T (0/1);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGT  >  NC_000913/1337153‑1337632
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               
aaGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAACGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGTACTGGGGTTTCACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTa                                                                                                                                                                                                                                         >  2:340862/1‑250 (MQ=255)
                                                gggCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTatcc                                                                                                                                                                                        >  1:365652/1‑251 (MQ=255)
                                                           ggCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGtt                                                                                                                                                                             >  1:262728/1‑251 (MQ=255)
                                                              aaCCATGGGTCAAAGCGGCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTaa                                                                                                                                                                          >  2:329338/1‑250 (MQ=255)
                                                                                          gggTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtgg                                                                                                                                             <  1:340862/251‑1 (MQ=255)
                                                                                                gAAAGTCGTTTCTGCTTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTg                                                                                                                                       >  2:87992/1‑251 (MQ=255)
                                                                                                       gttgcTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTAGTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATgc                                                                                                                                <  2:47252/247‑1 (MQ=255)
                                                                                                               ttatctcacAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTACCCTTGTGGGATACAGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGCTCCTATCGACACGGCAATCAAAAAAAGCGATTTCACCGTCGGTGCGTTGCTGTCCGGCAACCGTAACTTTAAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGGTGCCTATGCGCTGGCgg                                                                                                                        >  2:184039/9‑251 (MQ=255)
                                                                                                               ttATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCgg                                                                                                                        >  2:180953/1‑251 (MQ=255)
                                                                                                                     ggaaaaaGCGAAACGGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTAGCGAAACGGCAATCAAAAAAGGCGATTTACCCGTCGGTGCGGTGCTGTCCGGCAACCGTAACGTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACGGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAata                                                                                                                  <  2:57602/248‑1 (MQ=255)
                                                                                                                     ggCAAAAGCGAAACTGACACCGTGTCTCGACGAACTGGGGTGTAACCTTGTGGGATACGGTTGTCCCACCTGTATTGGTAACTCTGGGTCTCTGCCGGATTCTAGCGAAACGGCAATCAAAAAAGGCGATTGAACCGGCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGTTAGAACCTAACGGGCTGGCCTCGCCCCCGCTGGTGGTTGCCTATGCGCTGGCGTGAAat                                                                                                                   <  2:454813/250‑1 (MQ=255)
                                                                                                                     ggCAAAAGCGAAACTGACACCGGATCTCGGCGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAata                                                                                                                  <  1:250709/251‑1 (MQ=255)
                                                                                                                                       acCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCGGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGGCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCtt                                                                                                <  2:234225/251‑1 (MQ=255)
                                                                                                                                                            gTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATc                                                                           <  1:70279/251‑1 (MQ=255)
                                                                                                                                                                     tgtgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATAATCGCAAAGGc                                                                   <  2:159577/250‑1 (MQ=255)
                                                                                                                                                                       tgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCt                                                                                              >  2:121184/1‑221 (MQ=255)
                                                                                                                                                                       tgGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCt                                                                                              <  1:121184/221‑1 (MQ=255)
                                                                                                                                                                         ggATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCGGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATAGCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATcc                                                              <  2:423601/251‑1 (MQ=255)
                                                                                                                                                                               ggTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGGCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTa                                                        <  2:179595/251‑1 (MQ=255)
                                                                                                                                                                                   gTACCACCTGTATTTGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGTCCGTATCCATCCGCTGGTTAAAACTAACGGGCTGGCCTCGCCGCCGCTAGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGCGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTg                                                    <  2:319076/251‑1 (MQ=255)
                                                                                                                                                                                    tACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTg                                                    <  2:353486/250‑1 (MQ=255)
                                                                                                                                                                                                          gggCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGCTCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGcacc                             >  1:255741/1‑250 (MQ=255)
                                                                                                                                                                                                             ccgctccccgctccTATCGAAACGGCAAACAAAAACTGCGATTTAACCGTCGGTCCGTTTCTGTCCGGCAACCGTAACTTGGAAGGCCGTATCCATCCGCTGGTTAAAACGAACTGGCTGTCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTTAAATATATCTGGCCATCGGCACAAGa                          <  2:252406/251‑1 (MQ=255)
                                                                                                                                                                                                                 tGCCCTATCCTATCGAAACTGCAAGCAAAAAAGGCGATTTAACCGTCGGTGCGTTGCGGTCCGGCAACGGTAACTTTGAAGGCCTTATCCAGCCGCTGGTTAAAACTAACTGGCTGGCCTCTCCGCCGTTGCGGGTTGCCTGTGCGCTg                                                                                                                            <  2:271707/149‑1 (MQ=255)
                                                                                                                                                                                                                 tGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGAGCGGTGCTGTCCGGCAAACGTACCTTTGAACGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTCGTTGCCTATGCGCTg                                                                                                                            >  1:271707/1‑149 (MQ=255)
                                                                                                                                                                                                                 ggCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGAGATCTGGCCATCGGCACAAGAAAtt                      <  2:433858/250‑1 (MQ=255)
                                                                                                                                                                                                                          cgaTCGAAACTGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGGGCGGCCCGGCATCCGTACCGGTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATTCGCTGGCGGGAAATATGAATACCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGGGATCCTGTTTATCTGAAAGATATCTGGCCATCTGCACAAGAAAtttttcgtgca             <  2:447654/249‑10 (MQ=255)
                                                                                                                                                                                                                                     gCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGCACAAGt  >  2:400179/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               
AAGTGTGCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAGT  >  NC_000913/1337153‑1337632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: