Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,332,570	G→A	G508D (GGC→GAC)	topA →	DNA topoisomerase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,332,570	0	G	A	97.6%	141.1 / NA	42	G508D (GGC→GAC)	topA	DNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (16/25);  minor base T (0/1);  total (16/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGC  >  NC_000913/1332324‑1332811                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTAc                                                                                                                                                                                                                                               <  1:119526/251‑1 (MQ=255)      aTTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCg                                                                                                                                                                                                                                          <  2:48303/251‑1 (MQ=255)             gTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTACAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGa                                                                                                                                                                                                                                    >  2:238412/1‑250 (MQ=255)             gTTTTGAGGGCTGGACAAAAGAGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGTACTTACACCAGCACAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCTACCATTCAGGATCGTGACTACGTGCGAGTAGaa                                                                                                                                                                                                                                   <  1:379904/251‑1 (MQ=255)                ttGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCTATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGTATCGTGACTACGTGCGAGTAGaaaa                                                                                                                                                                                                                                 <  2:457276/250‑1 (MQ=255)                   aTGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTAAGGATCGTGACTACGTGCGAGTAGAAAAtcgt                                                                                                                                                                                                                             >  1:123427/1‑251 (MQ=255)                            cAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGTCCATTCAGGATCGTGTCTACGTGCGAGTAGAAAATCGTCGTTTCTAt                                                                                                                                                                                                                    <  1:418051/251‑1 (MQ=255)                                     tGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGaaaa                                                                                                                                                                                                            >  2:133657/1‑250 (MQ=255)                                                cgtAAAGGCGATGAAGATCGCATCTGTCCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAATCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTTCGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAAt                                                                                                                                                                                                <  1:367867/251‑1 (MQ=255)                                                 gtAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATc                                                                                                                                                                                               <  2:160188/251‑1 (MQ=255)                                                     aGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGCACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTc                                                                                                                                                                                            >  1:24746/1‑250 (MQ=255)                                                             aaGATCGCATCTTACCAGCAGTTAAGAAGAGCGATGCTCGGACGCTCGTAGAACTTACTCCAGCCCAACACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGTATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGTTCGTCCTTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTTACTACGTGCGATTAGAAAATCGTCGGTTCTATGCGGAAAAAATGGGCGAAATCGTCAGCGATCGc                                                                                                                                                                                   <  1:248737/251‑1 (MQ=255)                                                                 tCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTg                                                                                                                                                                               >  1:195581/1‑251 (MQ=255)                                                                              gcagGTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCTCCGATCGCCTTGAAGAAAATTTCcg                                                                                                                                                                  <  2:197530/251‑1 (MQ=255)                                                                                     ataaAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTAGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGtt                                                                                                                                                            >  1:278516/1‑250 (MQ=255)                                                                                             taTGCTCTGACGCTCGTTGACCTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGACAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACt                                                                                                                                                    >  2:99673/2‑250 (MQ=255)                                                                                             gATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTa                                                                                                                                                   <  2:166708/251‑1 (MQ=255)                                                                                                           cGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCCGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGACGCCCTGTGCGTCGATCATTTCGACCATTAAGGATCGTGACTACGTGCGCGAAAAAAATCGCCGTTTCTATGCGGGAAAAATGGGCGAAATCGACACCGATCGCCTTACAGAAAATTACCGCGCGTTAATGAACTACAACTTTACCgcgc                                                                                                                                     >  1:344601/1‑251 (MQ=255)                                                                                                            gTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCgcgc                                                                                                                                     >  1:146730/1‑250 (MQ=255)                                                                                                                    tACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGaa                                                                                                                             <  2:295681/250‑1 (MQ=255)                                                                                                                      cacCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAAGTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAAc                                                                                                                          <  1:68061/251‑1 (MQ=255)                                                                                                                               agcACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGTCGAAAGCGTCACCGATCGCCTTGAAGAAAATTTCCGCGATTTAAt                                                                                                                                                         <  2:163890/211‑1 (MQ=255)                                                                                                                               agcACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGCGTTAAt                                                                                                                                                         >  1:163890/1‑211 (MQ=255)                                                                                                                                  ccTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTTCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCTACCAg                                                                                                              <  2:302832/250‑1 (MQ=255)                                                                                                                                       aCCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGc                                                                                                         >  1:356789/1‑251 (MQ=255)                                                                                                                                           atccgccAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAAGCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATTAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAAt                                                                                                     <  1:297312/249‑1 (MQ=255)                                                                                                                                                             gTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACAGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGaa                                                                                    >  1:367696/1‑250 (MQ=255)                                                                                                                                                                         tGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGAt                                                                       >  1:214766/1‑251 (MQ=255)                                                                                                                                                                          ggTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATc                                                                      >  1:164839/1‑251 (MQ=255)                                                                                                                                                                              aaaGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCATCGACCAGGTGGCAAATCAAGAAGCAGAGTGGCAAGCTGGACTGGATccatt                                                                  >  1:295831/1‑247 (MQ=255)                                                                                                                                                                                agagCTGGAAAAACGCGGTATCGGGCGGCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCActtct                                                                <  2:356789/251‑1 (MQ=255)                                                                                                                                                                                   gCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACTAGGTGTCAAATCACGAAGCAGAGTGGAAAGCTGTACTTGATCACTgcttct                                                             <  2:24746/251‑1 (MQ=255)                                                                                                                                                                                   gCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTtcttct                                                             <  1:363416/251‑1 (MQ=255)                                                                                                                                                                                          aaaCGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGAttt                                                      <  2:214766/251‑1 (MQ=255)                                                                                                                                                                                             cgcgGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGTCCATTCAGGAGCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCTATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACGGGATCACTTCTTCTCGGATTTCAc                                                   <  2:278516/251‑1 (MQ=255)                                                                                                                                                                                                     cGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGCCTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGt                                           >  2:101513/1‑251 (MQ=255)                                                                                                                                                                                                            ccgtcTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTGTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATa                                     <  2:302450/250‑1 (MQ=255)                                                                                                                                                                                                              gtcTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAg                                  <  1:81449/251‑1 (MQ=255)                                                                                                                                                                                                                          gTCGATCATTTCGACCATTCAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATc                      <  1:129754/251‑1 (MQ=255)                                                                                                                                                                                                                                    tCGACCATTCAGTATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAgg             <  1:299565/250‑1 (MQ=255)                                                                                                                                                                                                                                             cAGGATCGTGACTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCTGAAGAGGGTGGTATgc    <  2:108749/250‑1 (MQ=255)                                                                                                                                                                                                                                              aGGATCGTGACTACGTGCGAGTAGAAAATCGGCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGGTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATgcgc  <  1:232188/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGGTTAAAGAGCTGGAAAAACGCGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCGTGGCTACGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATTTCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGGAAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTATGCGC  >  NC_000913/1332324‑1332811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑