Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,736	T→G	S325A (TCC→GCC)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,736	0	T	G	100.0%	74.5 / NA	27	S325A (TCC→GCC)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/14);  total (13/14)

TGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACAT  >  NC_000913/1306500‑1306972                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             tGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTg                                                                                                                                                                                                                                >  1:53133/1‑251 (MQ=255)           tgatgaGGTCTCCCACAATCCACTACATCCTTACACCAGGGCATTGAGGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACGGAAAGGGGAATTACCGCCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTAGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGGCCTGTGCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGAt                                                                                                                                                                                                                       <  2:69332/250‑1 (MQ=255)            gatgaGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGGCGGCAGTCCCCATACCTGATCCGGATCTGGAAAAGAACAAAACCATCCGGTTACTGGAAGGGGAATTACCGGCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCGGATTGCCGGTCCAGGGTGCGCCAAAACACGTCCTGTTGGGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATc                                                                                                                                                                                                                      <  1:32492/250‑1 (MQ=255)                          caATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCCGTTACTGGCAGGGGAATTACCGTCGCCGATCAACCCGCCTTACGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTAGATCCGCTTTAAAAACAAt                                                                                                                                                                                                       >  1:463492/1‑251 (MQ=255)                                   aCATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCt                                                                                                                                                                                                <  1:206911/249‑1 (MQ=255)                                           acacCAGGGCATAGATGTCGGCAGTCCCCCTACCTGCTCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCATGTTCTGGCGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTc                                                                                                                                                                                      >  2:323382/1‑251 (MQ=255)                                                    cATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCtttt                                                                                                                                                                             >  2:414456/1‑251 (MQ=255)                                                      atgatgtctgtatgccccATACCTTATCCGGATCTGGACACGAACAAACCCCTCCATTTGCTTTAAGTGGAGGTTCCGACGCCGAGCAATCCTCCGTTCGGTTGTTTTTTCCTGCCCCTTTGCCCTATTGCCGGTCCAGATTGCGCCAATCCACTGCCTGTTCTGGAGTGGATTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTATAATCATTAAGGGCTGACAGTTGTTATCTCTTTTTc                                                                                                                                                                           <  2:324522/237‑1 (MQ=255)                                                                                      tCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGcc                                                                                                                                               >  1:16308/1‑247 (MQ=255)                                                                                         ggAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCCGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAgatgat                                                                                                                                        >  1:75302/1‑251 (MQ=255)                                                                                                  cAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGAGATTCTTTATTCCCGCCAGATGATATAGCaa                                                                                                                                 >  1:393997/1‑249 (MQ=255)                                                                                                    aaaCCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGtt                                                                                                                             >  2:72145/1‑251 (MQ=255)                                                                                                                 aCTGGAAGGGGAATTTCCTTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGAACCCGTTGCCCGATTGCCGTTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTTTTCTTTTTc                                                                                                                <  1:465881/251‑1 (MQ=255)                                                                                                                             aTTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAg                                                                                                    <  1:414456/251‑1 (MQ=255)                                                                                                                                                    cTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTg                                                                              >  1:203689/1‑250 (MQ=255)                                                                                                                                                                 tttCCGTCCCCGTGGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCtt                                                                 <  1:52489/250‑1 (MQ=255)                                                                                                                                                                       tACCCGTCGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTCTCTTGCATGAACGTCGAACCGCTTTAAAACCAATAAGGGCGGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGCTTATTTATTCCCGCCCGATGATATGGCACAGTTTGTGCTCTTTTTAGCGGCATAACAGAATGCGGGCACAAACATCGTTGCTTTCACCATCTTCACTGTc                                                          >  2:288176/1‑251 (MQ=255)                                                                                                                                                                        aCCCGTTGCCCGCTTGCCGGTCCATATGGCGCCAAAACACGGCCTGTTCTGGAGGGGAGTTTAAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGCCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCACAAACATCGTTGATTTCACCATCTTCACTGTcc                                                         <  2:319987/251‑1 (MQ=255)                                                                                                                                                                          cctttGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGc                                                       <  2:341859/248‑1 (MQ=255)                                                                                                                                                                                                            acccGTCCTGTTTTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCggg                     <  2:203689/248‑1 (MQ=255)                                                                                                                                                                                                                gTCCTGTTCTGGAGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTc                  >  1:57143/1‑250 (MQ=255)                                                                                                                                                                                                                         gacgctctcccgatttacacGCCGGTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAAt        <  2:256600/235‑1 (MQ=255)                                                                                                                                                                                                                           gAGGGGATTTTAAGACACGCCGTTTCTTTCGTGAAAGTCGATCCGCTTTAAAAACAATAATGGCTGACAGGTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGAGTTCACCAGCTTCACTGTCCGCCAGACCAAGCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGaaagca      <  2:58283/251‑4 (MQ=255)                                                                                                                                                                                                                            aGGGGAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCa      >  1:156249/1‑250 (MQ=255)                                                                                                                                                                                                                               ggAGTTTCAGGCACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGGCAGTCGTTTTTCACGCTAAAAGCGATTATGTATTCCCGCCAGAGGTTATGGCAAAGTTTGCGAGCTTGTTCGCGGCATAACAGAATGGGGGCAAAAATATCGTTGATTGCACCACCATCGCGGTCCGCCAGACCAAACACCTCTGCGGCAAAAAAGCCCGGCTTGAATTCGAAATGAACAt  <  2:463492/251‑1 (MQ=255)                                                                                                                                                                                                                               ggAGTTTCAGACACGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACAt  <  1:72145/251‑1 (MQ=255)                                                                                                                                                                                                                                         acacGCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAAt        >  1:256600/1‑235 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             TGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAGGGGAGTTTCAGACACTCCGTTTCTTGCCTGAAAGTCGATCCGCTTTAAAAACAATAAGGGCTGACAGTTGTCAGCCCTTTTTCACGCTAAAAGCGATTATTTATTCCCGCCAGATGATATGGCAAAGTTTGTGATCTTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCCGCCAGACCAATCACCACTTCGGCAAAAAAGTCCGGGTTCAAATCGAAATCAACAT  >  NC_000913/1306500‑1306972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑