Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	100.0%	124.9 / NA	39	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/23);  total (16/23)

AACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGC  >  NC_000913/1301748‑1302231                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                aaCTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATTGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAACACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                            >  1:367993/1‑250 (MQ=255)      cACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATGCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCAt                                                                                                                                                                                                                                       <  2:355944/250‑1 (MQ=255)       aCCGGTGCCAAAAGACGCTATCGAGAAATTCGGCGCAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGc                                                                                                                                                                                                                                     >  2:329787/1‑251 (MQ=255)                      cGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTcc                                                                                                                                                                                                                      <  1:400315/251‑1 (MQ=255)                                     cGGCGAAAAATGGACCCAGCCTGGTAATATCGTAACCAACGGTGCCTATCCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaaaaa                                                                                                                                                                                                         >  1:165473/1‑249 (MQ=255)                                           aaaaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGTCCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCTCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAATCTGAAAAAAgagagcc                                                                                                                                                                                                 <  2:323234/251‑4 (MQ=255)                                                ggACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGAGTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTAGTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGAc                                                                                                                                                                                            <  2:165473/251‑1 (MQ=255)                                                            gTAATAGCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGGCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTAGTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGtt                                                                                                                                                                                <  2:278526/251‑1 (MQ=255)                                                                   cGTCACCAACGGGGCCTATTCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGTCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGTCGTATAACAGCATGCCGAACGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGTCGAAGTTCTCGTCGATCCa                                                                                                                                                                          <  2:190705/250‑1 (MQ=255)                                                                          aaCGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtgtg                                                                                                                                                                  >  1:182827/1‑251 (MQ=255)                                                                                    ataCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTa                                                                                                                                                         <  1:152585/250‑1 (MQ=255)                                                                                                           aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAAcc                                                                                                                                 >  1:356868/1‑251 (MQ=255)                                                                                                                  gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTAt                                                                                                                                                        >  1:369188/1‑221 (MQ=255)                                                                                                                  gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTAt                                                                                                                                                        <  2:369188/221‑1 (MQ=255)                                                                                                                                         aCCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGCAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGACGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGCAACCGCCATTCAACGATGTGCGTGTGCGTACCGc                                                                                                   >  2:322090/1‑251 (MQ=255)                                                                                                                                             aCTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCTCTACCGTAGTGGTGAAACCGACATGACGTATAACAGCATGCCGTTCGAATTGTGCCAGAAGCTGAAAAAAGAGATCCCGGGCGAGGTTCGCGTTGATCCATTCCTGTGGACTTACTATTACTAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACt                                                                                                <  1:440675/250‑1 (MQ=255)                                                                                                                                               tGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGa                                                                                              >  2:187750/1‑250 (MQ=255)                                                                                                                                                        gcgAAAACCGTTATTAACCAGGTAACCTATTTGCCTAGTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTAt                                                                                    <  1:65280/251‑1 (MQ=255)                                                                                                                                                           aaaaCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGCCAACCGCTACCGTAGTGGTGAAATCGACAGGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGa                                                                                 <  2:35521/251‑1 (MQ=255)                                                                                                                                                             acccGTTATTAACCAGGTAACTGATTTGCCTATTGCTGCGGAAGTTACCGATGCCAACCGCTACCGTAGTGGTGAAATCGAAATGACGTACAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGGGCACTTACTATTACAAAATTAACAACCAGAAACCGCCAGTCAACGATGTGCGTGTGCGTACCTCACTGAAACTAGGGATGGAcc                                                                               <  2:39298/249‑1 (MQ=255)                                                                                                                                                               ccGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACcgc                                                                             >  1:383465/1‑251 (MQ=255)                                                                                                                                                                   tattaACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGat                                                                          <  2:163292/250‑1 (MQ=255)                                                                                                                                                                      taACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTCCCGTAGGGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAATTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATc                                                                       <  1:322090/250‑1 (MQ=255)                                                                                                                                                                         caaGGTAACCTATTTGCTTATTGCTTCTGAAGTTTCCGATGTCAACCGCTACCGTAGTGGTGAAACCGACATGACGTATACCAGCATGCCTATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATCCCTGTGCCCTTACTATTACGAAATTAACACCCAGAACCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAACCTAGGTATGGACCGCGATATCATTg                                                                   <  2:338842/249‑1 (MQ=255)                                                                                                                                                                               aaCCTATTTGCCTATGTCTTCGGAAGTTACCGATGTCACCCGCTACCGTAGTTGTGAAATCGACATGACGTATAACAGCATGCCTATCGAATTGTCCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTCCTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTtaata                                                             <  1:166394/251‑1 (MQ=255)                                                                                                                                                                                    aTTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTg                                                        <  1:385858/251‑1 (MQ=255)                                                                                                                                                                                    aTTTGACTATTGCTTCTGACGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGACAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAAAAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGTACCGCGATATCATTGTTAATAAAGTg                                                        >  2:410556/1‑251 (MQ=255)                                                                                                                                                                                      ttGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCAGTGTTAATAAAGTGa                                                       <  1:135240/250‑1 (MQ=255)                                                                                                                                                                                              tGCTTCTGCAGTTACCGATGTCAAACGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAgg                                              >  2:325916/1‑251 (MQ=255)                                                                                                                                                                                                  tCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCa                                           >  2:221599/1‑250 (MQ=255)                                                                                                                                                                                                     gAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACa                                        <  1:325916/250‑1 (MQ=255)                                                                                                                                                                                                                          tACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGc                                            <  2:155086/225‑1 (MQ=255)                                                                                                                                                                                                                          tACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGc                                            >  1:155086/1‑225 (MQ=255)                                                                                                                                                                                                                            ccGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCgg                                                                                                                                                                                              <  2:131952/77‑1 (MQ=255)                                                                                                                                                                                                                            ccGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCgg                                                                                                                                                                                              >  1:131952/1‑77 (MQ=255)                                                                                                                                                                                                                             cGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCAcc               >  2:184758/1‑251 (MQ=255)                                                                                                                                                                                                                                      gAAATCGACATGACGTATAACAGCATTCCGATCGAATGGTCCAAGAAGCTGAAAAAAGAGACCCCGGACGAAGTTCACGTTGATCCATACCGGTGCACTTACTATTACAAAATGAACAACCAGAAACCGCCATTCAACGTTGTTCGTGTGCGGACCGCACTGAAACTAGGTATGGACCGCGATATCATTTTTAATAAAGTAAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGa      <  1:410556/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaTCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCCTTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATg    >  2:376295/1‑251 (MQ=255)                                                                                                                                                                                                                                          ccGACATGACGTATAACGGCAGGCCGATCAAATTGTTCCAGAAGCAGAAAAAAGAGAACCCGGACGAAGTTCACGTTGACCCATCCCTGTGCTCTTACTATTACGAAATTAACACCCAGAACCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGTCCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGgc  <  1:14740/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                AACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGC  >  NC_000913/1301748‑1302231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑