Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	31,683	C→T	N289N (AAC→AAT)	carB →	carbamoyl‑phosphate synthetase large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	31,683	0	C	T	100.0%	215.7 / NA	68	N289N (AAC→AAT)	carB	carbamoyl‑phosphate synthetase large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/37);  total (31/37)

CGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAAC‑TTCGAAAAATTCGCCGGTGC  >  NC_000913/31442‑31925                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      cGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGAGGTTCCAACGTTCAGTTTGCGGTGAATCCGaaaaa                                                                                                                                                                                                                                               >  1:258137/1‑250 (MQ=255)        cGGCTGGAAAGAGTACGAGATGGAAGTGGTTCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCATCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATTGCGGTGCTGCGTGAAATCTGCTTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGgtcgt                                                                                                                                                                                                                                        <  2:458793/250‑1 (MQ=255)          gCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCCCTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTg                                                                                                                                                                                                                                     <  2:101838/251‑1 (MQ=255)           cTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTg                                                                                                                                                                                                                                     >  2:23607/1‑250 (MQ=255)                aaGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACGGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTAGTCTGATTGtt                                                                                                                                                                                                                               <  1:252758/251‑1 (MQ=255)                   agTACGAGATGGACGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATc                                                                                                                                                                                                                            >  2:436917/1‑251 (MQ=255)                      aCGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGGCTGATTGTTATCGa                                                                                                                                                                                                                          <  1:376858/250‑1 (MQ=255)                       cGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACGTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGAGTGTTATCGaaa                                                                                                                                                                                                                        <  2:57267/251‑1 (MQ=255)                          gaTGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGGCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCTGGGAATCCGAAAAACGGGCGTCTGATTGTTATCGAAATGa                                                                                                                                                                                                                     <  2:178143/251‑1 (MQ=255)                            tGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAAc                                                                                                                                                                                                                   >  1:102855/1‑251 (MQ=255)                             ggAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAAcc                                                                                                                                                                                                                  <  1:100076/251‑1 (MQ=255)                             ggAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAAc                                                                                                                                                                                                                   <  2:134752/250‑1 (MQ=255)                              gAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGGGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAAccc                                                                                                                                                                                                                 <  1:364099/251‑1 (MQ=255)                               aaGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACAGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAACTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGTAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGACTGTTATCGAAATGAAcac                                                                                                                                                                                                                 >  1:163988/1‑248 (MQ=255)                                   ggtacGTGAAAAAAACGAGAACTGCAGCATCATCTGCTCTATCGAAAACGTCGAGTCGATGGGCAGCCACACCGGTGACTCCATCTCTGTCGCGCCAGCCCGAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCGCGATGGCGGTGCTGCGTGAAAACGGCGTTGAAACCGGTGGTTCCAACGTTCAATTGGCGGTGAATCCGAAAAACTGTCGTCTGATTGTAATCCAAAGGAAACCTcgcg                                                                                                                                                                                                            <  2:265172/247‑1 (MQ=255)                                    gtgCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCACCGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCgt                                                                                                                                                                                                           >  2:137829/1‑251 (MQ=255)                                      gCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGTGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGAGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCCCGCgtgt                                                                                                                                                                                                         >  2:216999/1‑251 (MQ=255)                                       cGTGATAAAAACGACAACTGCATCAGCGTCTGCTCTATCGAAAACTGCGATGCGATGGGCATCCACACCGGTGACACCATCTCTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCTGTGCTGCGTTAAATCGGCGGTGAAACCGGTGGTGTCAACGTTCAGGTTGCGGTGAATCCGAAAAACGGTCGTCGGATTGTTATCGAAATGATCCCACGCGTGTc                                                                                                                                                                                                        <  2:446654/251‑1 (MQ=255)                                              aaaaCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACTCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttct                                                                                                                                                                                                 <  2:122969/251‑1 (MQ=255)                                                aaCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGttcttc                                                                                                                                                                                               >  1:220158/1‑251 (MQ=255)                                                 aCGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCg                                                                                                                                                                                              >  1:450771/1‑251 (MQ=255)                                                          gCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTgg                                                                                                                                                                                       <  1:414778/249‑1 (MQ=255)                                                          gCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCg                                                                                                                                                                                     >  1:345485/1‑251 (MQ=255)                                                            atcatcGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTc                                                                                                                                                                                   <  1:261087/251‑1 (MQ=255)                                                            atcatcGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTc                                                                                                                                                                                   >  1:338750/1‑251 (MQ=255)                                                              catcGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCg                                                                                                                                                                                  >  1:209704/1‑250 (MQ=255)                                                                 cGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                                                                                               <  1:216999/250‑1 (MQ=255)                                                                  gTCTTCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGTCGTTGAAAGc                                                                                                                                                                             <  2:434344/251‑1 (MQ=255)                                                                    cTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCg                                                                                                                                                                            >  1:441835/1‑250 (MQ=255)                                                                     tGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAc                                                                                                                                                                          >  1:247510/1‑251 (MQ=255)                                                                     tGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAc                                                                                                                                                                          >  1:209970/1‑251 (MQ=255)                                                                          tATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGtt                                                                                                                                                                     >  1:4889/1‑251 (MQ=255)                                                                             cGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGTTTTcc                                                                                                                                                                  >  2:8423/1‑251 (MQ=255)                                                                              gAAAACGTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCGCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTccc                                                                                                                                                                 <  2:209704/251‑1 (MQ=255)                                                                                aaaCTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCg                                                                                                                                                                >  1:94772/1‑250 (MQ=255)                                                                                               gggCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGTTTGCTAAAGTggcgg                                                                                                                                                >  2:35764/1‑251 (MQ=255)                                                                                                          ccGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAAATGGCg                                                                                                                                     >  1:95414/1‑251 (MQ=255)                                                                                                           cGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACt                                                                                                                                         <  1:414461/246‑1 (MQ=255)                                                                                                           cGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCgg                                                                                                                                    <  2:209970/251‑1 (MQ=255)                                                                                                           cGGTGAATCCATCACTGTCGCGCCAGACCAAACGCTGACCGACAAAGAATATCAAATCATGAGTAACGCCTCGATGGCGGTGCTGCGTGAACTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGGGaaaatagatt                                                                                                                                    >  2:414461/1‑244 (MQ=255)                                                                                                            ggTGACTCCATCCCTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCggg                                                                                                                                   >  2:241369/1‑250 (MQ=255)                                                                                                                    cATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCTGTTCTGCGGCGCTGGCGTCGAAAGCTACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTaca                                                                                                                          <  1:238363/251‑1 (MQ=255)                                                                                                                          tGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATAAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGGAAATGGCgggg‑gggttaaccctcc                                                                                                                    >  2:446384/1‑240 (MQ=255)                                                                                                                           gTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGGTTCCCGATTGCTAAAGTGGCGGCGAAAATGGCGGTG‑GGGTGCACCCTcgg                                                                                                                   >  1:328364/1‑250 (MQ=255)                                                                                                                             cgcgCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTcgac                                                                                                                  <  1:82260/250‑1 (MQ=255)                                                                                                                               cgcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGaa                                                                                                               <  1:23607/251‑1 (MQ=255)                                                                                                                                gcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAAc                                                                                                              <  2:461480/251‑1 (MQ=255)                                                                                                                                  cAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCt                                                                                                                                                                                         >  1:398689/1‑175 (MQ=255)                                                                                                                                  cAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGGCTGATTGTTAGCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCt                                                                                                                                                                                         <  2:398689/175‑1 (MQ=255)                                                                                                                                       cAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCCAAACTGGCGGTGTGGTTA‑ACCCTCGACGACCtgatgg                                                                                                        >  1:58219/1‑249 (MQ=255)                                                                                                                                                 ccGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCACt                                                                                             <  1:12015/251‑1 (MQ=255)                                                                                                                                                  cGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGG‑GGGTTCACCCTCGAGGAACtgatggaaggcctcactg                                                                                            >  1:168382/1‑238 (MQ=255)                                                                                                                                                  cGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTGCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCAcgg                                                                                            <  2:120946/251‑3 (MQ=255)                                                                                                                                                      aaaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCACTGGCgg                                                                                        <  2:345485/251‑1 (MQ=255)                                                                                                                                                        aGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGAc                                                                                      <  1:137829/251‑1 (MQ=255)                                                                                                                                                         gAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACg                                                                                     <  1:355618/251‑1 (MQ=255)                                                                                                                                                            tatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGG‑GGGTACACCCTCGACGAACTGATGAAccaaatccccggcgggcggac                                                                                  >  1:56510/1‑231 (MQ=255)                                                                                                                                                               cAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGG‑GGGTTCACCCCCCACGAACTGATGAACGACATCACTGGCgggcgggccac                                                                               >  1:194490/1‑242 (MQ=255)                                                                                                                                                                              gCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGcc                                                               <  1:51263/251‑1 (MQ=255)                                                                                                                                                                                  cGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAACCGGTCGTCTGATTGTTATCGAAATGATCCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGt                                                             <  1:4000/249‑1 (MQ=255)                                                                                                                                                                                                gggtaaaTCGGCTGTGGAACCGGTGGCTCCATCGATCAGTTTGCGGGGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCGAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGTACTGATGAACGACATCACTGGCGGGCGGGCT‑CCGGTCTCGGTCGAGACGTCCATCGACTATGTGGt                                             <  2:196698/247‑1 (MQ=255)                                                                                                                                                                                                     aaTCGGCGTTGAAACCGGTTGTTCCAACGATCAGGTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCCCGCGTGTCCCGTTCTTCGGCGCTGGCGTCTAAAGCGACCTGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATTAACGACATCACTGGCTGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGTTTACTa                                        <  2:48250/251‑1 (MQ=255)                                                                                                                                                                                                      aTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCTTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCACTGTCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTAcgaa                                       <  2:37383/251‑4 (MQ=255)                                                                                                                                                                                                          gCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAGCGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAACCTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCACTTGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAAtt                                   <  2:168382/251‑1 (MQ=255)                                                                                                                                                                                                            gTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTcc                                 <  1:8423/251‑1 (MQ=255)                                                                                                                                                                                                                         gtTCCAACGTTCAGTTTGCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTT‑GGTTTCACCCTCGACGAACTGCTGAACGCCATCACTGGCGGGCGTACT‑CCCGCCTCCTTCTGGCCCACCCCCCACTATTTTGGTACTACAATTCCTCGCTTCAAC‑TTc                   >  1:291457/1‑251 (MQ=255)                                                                                                                                                                                                                                          gCGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑c                                                                               <  1:399937/175‑1 (MQ=255)                                                                                                                                                                                                                                           cGGTGAATCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGG‑GGTTACACCCTCGACGAACTGATGAACGACATCACTGGGGGGCGGACTTCCGCCCCCCTT‑GAGCCGTCCCTCGACTATGTGGTTTCTTAAATTTCTCGCTTCAACCTTCGaaaatttgcccgggcc  >  1:461461/1‑239 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      CGCTGATCGGCTGGAAAGAGTACGAGATGGAAGTGGTGCGTGATAAAAACGACAACTGCATCATCGTCTGCTCTATCGAAAACTTCGATGCGATGGGCATCCACACCGGTGACTCCATCACTGTCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTG‑GGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAAC‑TTCGAAAAATTCGCCGGTGC  >  NC_000913/31442‑31925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑