Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,156,913	G→A	G46D (GGT→GAT)	ycfH →	putative metal‑dependent hydrolase YcfH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,156,913	0	G	A	100.0%	73.3 / NA	24	G46D (GGT→GAT)	ycfH	putative metal‑dependent hydrolase YcfH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/13);  total (11/13)

TACAGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCC  >  NC_000913/1156670‑1157119                                                                                                                                                                                                                                                    |                                                                                                                                                                                                               tACAGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTAttt                                                                                                                                                                                                          <  2:114734/250‑1 (MQ=255)       cATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACACCATTACCGGATTATTTACATATg                                                                                                                                                                                                   >  2:367160/1‑251 (MQ=255)                   gCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTa                                                                                                                                                                                       <  2:283825/251‑1 (MQ=255)                     ttCTCTTCACCGATCTTTTCCTGCGTATTGAGCATTCCCTGCAACCGGGCGTTGAGCTACCGGTTCCTCATCTTTAAGAGAGACATCCTGTTTTTCGTCTACTCACACTGCCATCTCGATGGTCTGGTTTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGAGAAATTTTGTCTGGAGGTCGCCACACCATTACCGGATTATTTACATATGCGGAATCTGGtcgg                                                                                                                                                                                     >  2:2749/1‑248 (MQ=255)                     ttCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTacg                                                                                                                                                                                     >  2:122534/1‑249 (MQ=255)                                     tttACTGCGTATTGAGCAGTACCTGCAACCGGGCGTTGGGCTACCGGTTCCGCATCTTTAAGAGATACATCATGTTTTTAGTCGACTCACACTGCCATCTCGAGGGTCTGGATTATGAATCTTTGCATAAGGACTTGGATGTCGTTCTGGCGAAAGCCTCCTCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAAc                                                                                                                                                                        <  1:221131/248‑1 (MQ=255)                                               aTTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCtt                                                                                                                                                           <  1:65246/251‑1 (MQ=255)                                                   aGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCTTATTTTCTTGTgg                                                                                                                                                       <  2:329218/251‑1 (MQ=255)                                                                   gggCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCCTGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAAc                                                                                                                                       >  2:304036/1‑251 (MQ=255)                                                                     gCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCa                                                                                                                                     <  1:255847/251‑1 (MQ=255)                                                                        ttGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCAGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCGCAACATTACCGGATTATTTACATATGCGGGATCTGGGAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTAAACCAGa                                                                                                                                   >  1:448615/1‑250 (MQ=255)                                                                                              ttAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGACTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGACGCCACAACCTTACCGGATTATTTCCATATGCGGGATCTGGTAGGCGAACGTGACATCGTCGTATTTTCTTGCGGCGTGCATCCACTCAACCCGAATGATCACTACGTTGTaga                                                                                                                >  2:325030/1‑247 (MQ=255)                                                                                                aaGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGAttt                                                                                                          >  2:206665/1‑251 (MQ=255)                                                                                                                            cacaCTGCCATCTCGCTGGTCTGGATTATGAATCTTTGCATAAGGACGTTGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGgtgt                                                                               <  1:135524/250‑1 (MQ=255)                                                                                                                              cacTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCCGAAGAGGGggttgt                                                                            >  2:365930/1‑251 (MQ=255)                                                                                                                               acTGCCAGCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGGGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGTATTATTTACATATGCGGGATCTGGTAGGCGAACGTTACAACGTAGTATTTTCTTGTTGCGTTCATTCACTCAACCGGAATGCTCCCTATGATGTAGAAGATTTACGCCGCCTGGCGGCAGAAGTGGGTTTTGTa                                                                           <  2:219025/251‑1 (MQ=255)                                                                                                                                   ccATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAgcgc                                                                       <  1:325030/251‑1 (MQ=255)                                                                                                                                            tGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGCCGGCAGAAGAGGGGGTTGTAACGCTGGGTgga                                                               >  1:265519/1‑248 (MQ=255)                                                                                                                                                          aaTCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGattat                                                <  1:206665/251‑1 (MQ=255)                                                                                                                                                                                                   gccgcACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTAAGTCAGCAAGAGTCCtt       >  1:2124/1‑251 (MQ=255)                                                                                                                                                                                                    ccgcACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAagag                                                                                    <  1:4587/173‑1 (MQ=255)                                                                                                                                                                                                    ccgcACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAagag                                                                                    >  2:4587/1‑173 (MQ=255)                                                                                                                                                                                                      gcACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGGACGTCAGCAAGAGTCCTTcat    <  1:98559/251‑1 (MQ=255)                                                                                                                                                                                                        aCGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGATTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTcatcc  <  1:304036/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                               TACAGGCATCAACCGCGAGCTTCTCATCACCGATCTTTTACTGCGTATTGAGCATTACCTGCAACCGGGCGTTGTGCTACCGGTTCCTCATCTTTAAGAGAGACATCATGTTTTTAGTCGACTCACACTGCCATCTCGATGGTCTGGATTATGAATCTTTGCATAAGGACGTGGATGACGTTCTGGCGAAAGCCGCCGCACGCGATGTGAAATTTTGTCTGGCAGTCGCCACAACATTACCGGGTTATTTACATATGCGGGATCTGGTAGGCGAACGTGACAACGTCGTATTTTCTTGTGGCGTGCATCCACTCAACCAGAATGATCCCTACGATGTAGAAGATTTACGCCGTCTGGCGGCAGAAGAGGGTGTTGTAGCGCTGGGTGAAACCGGGCTGGATTATTATTACACGCCGGAAACTAAAGTACGTCAGCAAGAGTCCTTCATCC  >  NC_000913/1156670‑1157119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑