Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,088,081	G→T	Q593K (CAA→AAA)	pgaB ←	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase and beta‑1,6 glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,088,081	0	G	T	100.0%	39.6 / NA	14	Q593K (CAA→AAA)	pgaB	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase and beta‑1,6 glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/8);  total (6/8)

ATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCTGTGCAAACCAGGCTTCACTTTCAGGTTGTATTACCGGAAGTGCAAAAATATTTCGTGCAGTTTTAATATGTGGACCGCGAATGGCTTTTACGCGCGCACTAAGTTCTAAAGTGAAGTCAGTTAAC  >  NC_000913/1087842‑1088319                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               aTCATTTTTCGGATAACAGGCTGTTGAACACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGATCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTTATTGGTCAAtt                                                                                                                                                                                                                                     >  2:262503/1‑251 (MQ=255)                                            aGGTCTATTACAGGTTGGTTATGCAGAAAATTGGCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTTAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGGCCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTTATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCGTCCAg                                                                                                                                                                                          <  2:103440/251‑1 (MQ=255)                                                            ggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTTATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATATCCata                                                                                                                                                                          <  1:68065/251‑1 (MQ=255)                                                               tATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTTATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCataat                                                                                                                                                                        <  1:277493/250‑1 (MQ=255)                                                                                    aataaCCATAGTGTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTTATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACGCCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCtttt                                                                                                                                                   <  1:18861/250‑1 (MQ=255)                                                                                                 tttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATGGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTGTATTGGTCAAGTGTATTAACCATTGTTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCa                                                                                                                                      <  1:240759/250‑1 (MQ=255)                                                                                                    cACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTTATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAAt                                                                                                                                  >  1:255371/1‑251 (MQ=255)                                                                                                                                   tGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTTATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCTGTGCAAACCAGGCTTCACTTTCAGGTTg                                                                                                   >  1:90882/1‑251 (MQ=255)                                                                                                                                             gttgCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTTATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCTGTGCAAACCAGGCTCCACTTTCAGGTTGTATTACCgg                                                                                          <  1:369843/250‑1 (MQ=255)                                                                                                                                               tgCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTTATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCTGTGCAAACCAGGCTTCACTTTCAGGTTGTATTACCGGa                                                                                         >  1:33769/1‑249 (MQ=255)                                                                                                                                               tgCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTTATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTGCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCTGTGCAAACCAGGCTTCACTTTCAGGTTGTATTACCGGaa                                                                                        <  1:54241/250‑1 (MQ=255)                                                                                                                                                            ccTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTTATTGGTCAAGTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCTGTGCAAACCAGGCTGCACTTTCAGGTTGTATTACCGGAAGTGCAAAAATAttt                                                                          <  2:181293/251‑1 (MQ=255)                                                                                                                                                                                           tgCCTGTAATTCTAAAATAGCTTTCTCTTTATACTGAGGGCTGTTTTTAATTTTACTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCAACACCTTCCAGATAAGGCATAGCCATAATAGCGATCCAGTCATAGCTTTTTAGGAAATCAGCATACTTCTGTGCAAACCAGGCTTCACTTTCAGGTTGTATTACCGGAAGTGAATAAATCTGTCGTGCAGTTTTACTATGTGGACCGCGAATgg                                           >  2:137056/1‑251 (MQ=255)                                                                                                                                                                                                                                    tGTTTTTAATTTTATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCTGTGCAAACCAGGCTTCACTTTCAGGTTGTATTACCGGAAGTGCAAAAATATTTCGTGCAGTTTTAATATGTGGACCGCGAATGGCTTTTACGCGCGCACTAAGTTCTAAAGTGAAGTCAGTTAAc  >  2:175347/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               ATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAGATAAGGCATAGCCATAATAGCGGTCCAGTCATAGCTTTTTAGGAAATCAGCATAATTCTGTGCAAACCAGGCTTCACTTTCAGGTTGTATTACCGGAAGTGCAAAAATATTTCGTGCAGTTTTAATATGTGGACCGCGAATGGCTTTTACGCGCGCACTAAGTTCTAAAGTGAAGTCAGTTAAC  >  NC_000913/1087842‑1088319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑