Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,069,743	C→T	A33T (GCA→ACA)	rutG ←	pyrimidine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,069,743	0	C	T	96.9%	92.4 / NA	33	A33T (GCA→ACA)	rutG	pyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (21/10);  minor base G (0/1);  total (22/11)
Fisher's exact test for biased strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGAC‑GCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGG  >  NC_000913/1069496‑1069989                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       ccGCCCAGCCCAATGCTGATATTCGGGTTAATGCCCGGACCGTAAAAGCCCGTCGCGGCGACCCCCGCGCCGAAAAAAGCCGCGCTGGAGCCGAAATAACTGGGGAC‑GCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGt                                                                                                                                                                                                                                                     <  1:218166/251‑1 (MQ=255)               gCTGATATTCGGGATAATGCCCTGACCGTTAAAGACCGACGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGAC‑GCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGCAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGc                                                                                                                                                                                                                                        >  2:303103/1‑250 (MQ=255)                 ggATATGCGGGTAAATGCCCGGACCGTTAAACCCCGCCGCGGCGACCCCCACGCCGACAAAAGCCGCGCGGGAGCCGAGATAACTGGGGAC‑GCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAAt                                                                                                                                                                                                                                     <  1:90647/250‑1 (MQ=255)                            ttaaggcccggcccGTAAAAGCCCGGCGCGGCACCCCCCACGCCGAAAAAAGCCGCGCTGGAGCCGA‑AAGACGGGGGCCCGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCg                                                                                                                                                                                                                           <  2:94336/238‑1 (MQ=255)                            ttAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGAC‑GCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCgg                                                                                                                                                                                                                          >  1:213165/1‑251 (MQ=255)                                   ccTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGAC‑GCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGAcc                                                                                                                                                                                                                   >  1:1361/1‑251 (MQ=255)                                      gaccgtaaaacccggccggggcaaccaccccGCCGACAAAAGCCGCGCTGGAGCTGAGAAAACGGGGGCC‑GCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCAc                                                                                                                                                                                                                 <  1:22899/225‑1 (MQ=255)                                        ccGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGCGATAACTGGGGAC‑GCGACCGCAGGTAATAAAGACAAACAGTAACGTGCCGATCCCCGCCATAAAAATGGAAAGATTGGGATCCAGCCACATCAGAATCGGCATCAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCCTCCGGCGCGACCAcgcc                                                                                                                                                                                                              >  1:47097/1‑251 (MQ=255)                                              aaGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGAC‑GCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGCGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGctctc                                                                                                                                                                                                        >  2:162844/1‑251 (MQ=255)                                                            gATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGAC‑GCGCCCGCCGGTAATAAAGCAAAACAGTAACGTGCCGATCCCCGACATTCAAATGGAAAGATTGGGATCAAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGCGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCgctt                                                                                                                                                                                          >  1:461286/1‑248 (MQ=255)                                                                accacGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGAC‑GCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTa                                                                                                                                                                                       >  1:114157/1‑250 (MQ=255)                                                                                       ggagcggagaaagggggggAC‑CCGCCCCCCGGAAAAAAAGAAAAACAGTAACGGGCCGATCCCCGACATAAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCAAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTTTGAGCAAACGGCAATCGTTCATCCGGCGCGCCCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACa                                                                                                                                                               <  1:208119/236‑1 (MQ=255)                                                                                                                      ggTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGACATCAGCTGTCTCCTTAAGGAGGtt                                                                                                                                  >  1:205909/1‑250 (MQ=255)                                                                                                                        taataccgaaacacaataccgcgCCGATCCCGGACATTAAAATGGAAAGATAGGGATACAGCCCCATCAGCCTCGGCATCAACACACTCGCGCCCAAAATCGCCACCACATGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGAGAGGAACACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTacc                                                                                                                               >  2:305913/23‑249 (MQ=255)                                                                                                                        taataaAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGCGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAAc                                                                                                                               >  2:186392/1‑251 (MQ=255)                                                                                                                                   aaCAATAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTAGACACAAATATCGCAACCGCGTGTTGAACCCCAATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACAACGCCGCTCTCTGAAGAGGTCGATTTTAACTGTCAGACCGGGAAAACGAACAGTGCCATCAGCTGTCTCCTTAAGGAGGTTAAAAAgacaggagg                                                                                                                    >  1:256263/1‑243 (MQ=255)                                                                                                                                             tGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGt                                                                                                          >  1:214675/1‑251 (MQ=255)                                                                                                                                                    ccccGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCCCCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACt                                                                                                   <  1:439079/251‑1 (MQ=255)                                                                                                                                                             tAAAATGGAAAGCTTGGAATCCAGCCCCATCAGAATCGGAATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGCGGAAAACCGAACATTGCCATCCGCTGTCTCATTACGGAGGTTAACATGCAGGGCGCATCAGCGCGTGATAACTGCGCTCgc                                                                                           >  2:367906/1‑249 (MQ=255)                                                                                                                                                             aaaaaTGGAAAGATTGGGATCCCGCCCCAGCAGAACCGGCCTTAACACCGTCGCCCCAAACACCTCCCCCGCTTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGaa                                                                                          <  2:395054/250‑1 (MQ=255)                                                                                                                                                                   ggAAAGATTGGGATCCAGCCCCATCAGAACCGGCATTACCCCCGTCGCCCCAAACATCGCCACCGCGTGTTAACCGCCCATTACTGGCGTCGGAGCAAGCGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCATGGCGCATCAGCGCGTGATAACTGCGATCGAACCacac                                                                                    <  2:407978/251‑1 (MQ=255)                                                                                                                                                                            gggatcacgccacATCAGAATAGGCATTAACACCGTCGCGCCAAACATCGACACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGt                                                                            >  2:67334/13‑250 (MQ=255)                                                                                                                                                                              gATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCAGTAgg                                                                         >  2:250287/1‑251 (MQ=255)                                                                                                                                                                                      cccATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAag                                                                                                                                        >  1:365863/1‑180 (MQ=255)                                                                                                                                                                                            aGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGCACGCCCATTACTGTCGTCTGAGCCAACGCCAATAGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGACAACCGAACATTGCCATCAGCTGTCTCCTTCAGGAGGTTAACAAGCAGGGCGCATCCGCGCGTGATACCTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGa                                                              >  1:23850/1‑248 (MQ=255)                                                                                                                                                                                             gAATCGGCATTAACACCGTCGCGCCAAAAATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCCTCCGGCGCGAACACGACGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGATGTCTCCTTAAGGAGGTTACAAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGAt                                                          >  2:449720/1‑251 (MQ=255)                                                                                                                                                                                                 cGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCACACGGCAATCGTTCATCAGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGACTCCTTAAGGAGGTTAACAAGCAGGGCGCCTCAGCGCGTGATACCTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAAt                                                      >  1:464860/1‑251 (MQ=255)                                                                                                                                                                                                    cATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGc                                                   >  2:211913/1‑251 (MQ=255)                                                                                                                                                                                                     aTTAACACCGTCGCGCCCAACATCGCCACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCACACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAAc                                                                                                                                                                >  2:268204/1‑141 (MQ=255)                                                                                                                                                                                                     aTTAACACCGTCGCGCCAAACATCGCCCCCGCGTGTTGAACGCCCATTACTGTCGGCGGAGCAAACGGCAAGCTTTCATCCGGCGCGTCCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAAc                                                                                                                                                                <  1:268204/141‑1 (MQ=255)                                                                                                                                                                                                                         cATCGCCCCCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTCCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAAt                              <  1:303103/251‑1 (MQ=255)                                                                                                                                                                                                                                 ccGCGTGTTGACCTCCCATTATGGTCGTCTGAGCAACCGGCAATCGTTCAGCCGTCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTggg                      <  2:240393/251‑1 (MQ=255)                                                                                                                                                                                                                                                     aCTGTCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTgg  >  2:362064/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       CCGCCCAGCGCAATGCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGAC‑GCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCCACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCATCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACGCTCACCACCTGG  >  NC_000913/1069496‑1069989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑