Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,240	G→A	L21L (TTG→TTA)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,240	0	G	A	100.0%	105.0 / NA	31	L21L (TTG→TTA)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/18);  total (13/18)

AAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGC  >  NC_000913/4637993‑4638486                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       aaaGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATGGCTGTTGTTAAtt                                                                                                                                                                                                                                                     <  2:217896/251‑1 (MQ=255)                gggtGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTTCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGAtt                                                                                                                                                                                                                                      <  2:323275/248‑1 (MQ=255)                    ggCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCgg                                                                                                                                                                                                                                   <  2:179709/250‑1 (MQ=255)                                  gggTCGCACGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGaa                                                                                                                                                                                                                    <  1:11940/250‑1 (MQ=255)                                  aGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGaa                                                                                                                                                                                                                    <  2:405353/251‑1 (MQ=255)                                            gttgtttAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGTTGTCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATGCTTCCCACAGAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGTCTGGTTGGTGCAGTATTGCGGTTGTTAATTCCGATAATGCTGATTCGACAGGTGATTGTCGAACGGGCTGAtt                                                                                                                                                                                                          <  2:158534/250‑1 (MQ=255)                                                          aaGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGAtgtg                                                                                                                                                                                            <  1:153045/251‑1 (MQ=255)                                                                cGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCAGTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGAt                                                                                                                                                                                      <  1:196588/251‑1 (MQ=255)                                                                          cGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACCTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGcc                                                                                                                                                                            <  2:95171/251‑1 (MQ=255)                                                                                  aaGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTAcc                                                                                                                                                                    <  1:411654/251‑1 (MQ=255)                                                                                       ggCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATACTACTGCCATTGCAACGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAACATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATACTGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTCCCGTAGCGATGTGGAAGATGCGATTCGCCACAGTACCAGCgg                                                                                                                                                               >  1:231401/1‑251 (MQ=255)                                                                                                gCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTGCCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTGCCAGTGGGCCGCaaaa                                                                                                                                                      <  2:290810/251‑1 (MQ=255)                                                                                                                                     aaaTTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGACGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTACTTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCg                                                                                                                   >  1:213488/1‑249 (MQ=255)                                                                                                                                                acaTAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGt                                                                                                      >  2:324283/1‑251 (MQ=255)                                                                                                                                                     gTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGAtt                                                                                                                                                                                                          <  2:154438/146‑1 (MQ=255)                                                                                                                                                     gTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGAtt                                                                                                                                                                                                          >  1:154438/1‑146 (MQ=255)                                                                                                                                                                ctgctgCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGAGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATAGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATaa                                                                                       >  1:466676/1‑250 (MQ=255)                                                                                                                                                                       cATTGCAAAGGAGAAGCCTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTTGTGCAGTATTGCTGTTGTTAATTCCGATAATGGTGATTCGGCAGGTGATTGTCGATCGTCCTGATTCCCGTAGCGATGTGGGAGATGCGATTCGCCGAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCGGCTCATCGCTATTCCTGTTACCGAGCGTTATACGGTGCAGGAAGACGATAAACCCGTgg                                                                               <  2:449272/251‑1 (MQ=255)                                                                                                                                                                             aaaGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCgg                                                                          >  1:344342/1‑250 (MQ=255)                                                                                                                                                                                         tATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGtttt                                                              >  1:248966/1‑250 (MQ=255)                                                                                                                                                                                                     cccccTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATACGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGCAGATGCGATTCGCCAAAGTACCAGCGGGCAGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGt                                                 >  1:280261/1‑251 (MQ=255)                                                                                                                                                                                                       cccTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCCTCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGtt                                                >  2:384233/1‑250 (MQ=255)                                                                                                                                                                                                        ccTGTTCTTGAAAATGACTTGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCATGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTAc                                              <  2:18473/251‑1 (MQ=255)                                                                                                                                                                                                             tCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTTCCTg                                           >  1:236080/1‑249 (MQ=255)                                                                                                                                                                                                             tCTGGAAAATGACTAGCCTGTTTGGTGCAGTAATGCTGTGGGTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTGGGGCCGCGCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGTAAGAGGTTAAAACCGTGGAGCGGAAACGAAGGTTGATCCAGGTTTTGTTACCTGa                                          <  2:225916/250‑1 (MQ=255)                                                                                                                                                                                                                                   tGGTGAAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCCGGCGATTTTCGAACGTGCTGATTACCGTAGCGGTGTGGAAGATGCGATTCGCCAACGTACCAGCGGGCCGCAAAAACTAGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGGTTTATCCATTTTTGGTTACCTGGGTCATTGATGGATGATGGCAATc                   >  1:242670/1‑251 (MQ=255)                                                                                                                                                                                                                                    ggTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCa                  <  2:128017/251‑1 (MQ=255)                                                                                                                                                                                                                                           tATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTg           >  2:279859/1‑251 (MQ=255)                                                                                                                                                                                                                                            aTTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTgg          >  1:451661/1‑251 (MQ=255)                                                                                                                                                                                                                                               gCTGTTGTTAATTCCGGTAATGCTGATTCGGCAGGTGAGTGTCGAACGTGCTGATTACCGTAGCGATTTGGAAGATGCGATTCGCCAGAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTTTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGATTCATTGATGGTTGATGGCAATCAGAACGTGgaag       <  1:341465/251‑1 (MQ=255)                                                                                                                                                                                                                                               gCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGATCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGgaa        <  1:339886/250‑1 (MQ=255)                                                                                                                                                                                                                                                    tgttaATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGAGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGc  >  2:114104/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       AAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGC  >  NC_000913/4637993‑4638486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑