Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,604,517 C→T L120L (CTG→TTG)  bglJ → DNA‑binding transcriptional regulator BglJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,5170CT96.8% 88.9 / ‑6.3 31L120L (CTG→TTG) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (12/18);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTG  >  NC_000913/4604277‑4604757
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                
aCCTATTCCAGTCAGGAGTCATTTCAGGATGCCATGGCGCGGATCTCTTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTTAGCGCAGATAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGGATTAAGTAATGCGTCAACGTTGGAGAtttt                                                                                                                                                                                                                                        <  2:401690/251‑1 (MQ=255)
      aCCAGGCAGGCGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTtcatc                                                                                                                                                                                                                                   <  2:231891/250‑1 (MQ=255)
         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCCGCGAGGGGTTATCTTGGCTGACTGTACTTGCGGATTAAGTT‑CCGCGTACCCGGCGGTTTGTTATTGCGGATGATGGTATTGAAACCCGGCTGATTGGTTTATTGTCGCCATCTACGCTGGAAGGGGGATTAAGGAAAGCGTCAAAGCTGGAGGATTTtcatcacg                                                                                                                                                                                                                                >  1:299891/1‑248 (MQ=255)
                       tCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCa                                                                                                                                                                                                                  <  2:103133/250‑1 (MQ=255)
                                cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTg                                                                                                                                                                                                                     <  1:45292/238‑1 (MQ=255)
                                                                     ttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTg                                                                                                                                                                      <  1:39543/248‑1 (MQ=255)
                                                                                 agaAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTTATTTTCCGCGTACCCGGCGTTTAGTTATTGTTGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTTCATTAACCAAAg                                                                                                                                                       <  2:201429/251‑1 (MQ=255)
                                                                                          cgcAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGtt                                                                                                                                              >  2:437563/1‑251 (MQ=255)
                                                                                           gcAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGCTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGCTGATATTGAAGCTCGGCTGATTGGTTCATTGTCCCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGtt                                                                                                                                              >  1:194625/1‑250 (MQ=255)
                                                                                               agagGGATTATCTTGCCTGACGGAACTGGAG‑ATTAAGTTTCCGCGTAACCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTTATTGTCGCCATCATCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGAGTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTaa                                                                                                                                                                                                     <  1:394127/191‑2 (MQ=255)
                                                                                                   ggATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGt                                                                                                                                                                                                   <  1:184667/189‑1 (MQ=255)
                                                                                                   ggATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGCCGTTTAGTTATTGCGGATGATGCTATTGAAGCTCGGCTGATTGGTTCCTTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCACCGTTGGAGCTTTTTCATCAGGAACTCTTTTTGTCATTACATGGTGTACGt                                                                                                                                                                                                   >  2:184667/1‑189 (MQ=255)
                                                                                                    gATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACg                                                                                                                                    <  1:390036/251‑1 (MQ=255)
                                                                                                                   ctgaagtagCG‑ATTAAGTTTCTGCACACCAGGCCTTTAGTTATTGTGGATGCTGATATTGTAGCACGCCTGATTGGTTCATTGTCGCAATCACCGCTGGACGGAGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTCAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGCGAGAGAAATATTg                                                                                                                     >  2:278675/9‑251 (MQ=255)
                                                                                                                         ctggcgcctAAGCTTCCGAGTACCCGGCGTTTAGTTACTGCGGATGCTGCTATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTCTTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGAACGTCAGGCGACCGACCGCCTGAACAATCAGTGGCACATTAACCAAAGCCGGACGTTACGCCCGACGGAGAGAGGAATATTGGGCtt                                                                                                                >  2:298889/9‑251 (MQ=255)
                                                                                                                          ggCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTa                                                                                                              <  1:108822/251‑1 (MQ=255)
                                                                                                                          ggCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTa                                                                                                              <  2:462531/251‑1 (MQ=255)
                                                                                                                                            gTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTc                                                                                              >  1:83896/1‑250 (MQ=255)
                                                                                                                                                  ggCGTTTAGTTATTGCGGATGATGATACTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGTACGGTTTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGCGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGaca                                                                                       <  2:133461/251‑1 (MQ=255)
                                                                                                                                                        tAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAAt                                                                                  <  2:95627/250‑1 (MQ=255)
                                                                                                                                                         aGTTATTGCGGATGATGATATTGTAGCTCGGCTGATTGGTTCATTGTCGCCAGCACCGCTGGACGGTGTATTAAGTAAAGCTTCAACGTTGGAGATTTTTCGTCAGGAACTCATTTTGTCATTAAATGGTGTACGTCAGGCTACCGACCGACGGAACAAGCAGTGGTACATTAACCAAAGCTGGTCGTTAAGCCCGACGGATAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTg                                                                                <  1:420977/251‑1 (MQ=255)
                                                                                                                                                             aTTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGa                                                                            <  2:265332/251‑1 (MQ=255)
                                                                                                                                                                  ggATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGa                                                                                         <  1:323401/233‑1 (MQ=255)
                                                                                                                                                                  ggATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGa                                                                                         >  2:323401/1‑233 (MQ=255)
                                                                                                                                                                      gatgatATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGCGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTa                                                                    >  1:384801/1‑250 (MQ=255)
                                                                                                                                                                                                           cATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAAt                              >  1:363836/1‑251 (MQ=255)
                                                                                                                                                                                                             tCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGCTTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGTCCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTCAtgt                            >  2:183751/1‑251 (MQ=255)
                                                                                                                                                                                                                       aCGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGaa                   >  2:99112/1‑250 (MQ=255)
                                                                                                                                                                                                                                 aaGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTGTGGTCATTAAATGGTGTACGTCAGGCGGCCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGGGATTTCGAAACTGGGCGTc         <  2:384801/250‑1 (MQ=255)
                                                                                                                                                                                                                                    taaagaatCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAACGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGtt     >  2:310841/8‑251 (MQ=255)
                                                                                                                                                                                                                                       aGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTg  <  1:183751/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                
ACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCG‑ATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTG  >  NC_000913/4604277‑4604757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑