Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,571,280	C→T	E99K (GAG→AAG)	yjiR ←	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,571,280	0	C	T	100.0%	110.0 / NA	33	E99K (GAG→AAG)	yjiR	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/28);  total (5/28)

ATGGCAGCCGCTGGTGAT‑GATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACG  >  NC_000913/4571041‑4571514                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            cgggccgCCGCGGGTGAT‑GATGAGGTCTTCGGCGTGGACCACGGACCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATCCTCTCTGCCCGGCAACCAAGTATTAACCGAGAACGGTTTGCAGATTTCGCTGCACCACCCTGCTTATCTTCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATcc                                                                                                                                                                                                                                   <  2:335243/244‑1 (MQ=255)       gCCGCTGG‑GATTGATGATGTCACCGGGGGTGCCCACCGCGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCt                                                                                                                                                                                                                            <  2:335495/251‑1 (MQ=255)               ggat‑gaggatGTCATGGGCGGTGACCCCCGAGCGGCTGTCGGGCATCAGGCGGGCATTCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTTCAGATTAGGCGGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCTGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATAGCCAGCGCCTGATCCCAc                                                                                                                                                                                                                    <  2:455561/250‑1 (MQ=255)                             aagggcggGGACCACCGAGCGGCTGTCGAGCACCAGGCGGACAATCTGCTCTCGCAATACGCGCTGACCGGCTAACAAGTCATACCCGAGAACGTTTTGCAGATTATGCTGCGCCCCCCGGATTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCa                                                                                                                                                                                                       <  1:43210/248‑1 (MQ=255)                                       accaccGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTg                                                                                                                                                                                             >  1:428587/1‑251 (MQ=255)                                         ccccgggCCGCTGTCGAGCAGCGGGGGGGCAATCTGCTCTCGCAATACTCGCGGACCGGCTAAAAAGTCATAACCGAGAACGGTTTGCAGTTTATGCTGCACCACCCGGCTTAGCCCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCGGATCCCACTGGGTAATTTCCACCGGGCGCTGCa                                                                                                                                                                                           <  2:420399/245‑1 (MQ=255)                                         caccGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCa                                                                                                                                                                                           <  1:321814/251‑1 (MQ=255)                                            cgacccGCTGTGGAGCATAAGGCGGGCAATCTGCTCCCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCTCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCg                                                                                                                                                                                        <  2:432338/247‑1 (MQ=255)                                               gcggCTGTCGAGCATCGGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGAc                                                                                                                                                                                     <  1:116335/248‑1 (MQ=255)                                                                ggCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGCCGCGTCATcggcgggacc                                                                                                                                                                    >  2:169547/1‑247 (MQ=255)                                                                      cAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTAcctgcggc                                                                                                                                                              >  1:182498/1‑244 (MQ=255)                                                                       aaTCTGCTCTGTCAAACCTCTCTGACCGGATAGCAAGGCATAACCGAGATCGGTTTGCAGATTATGCTGCCCCTCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAAGTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCt                                                                                                                                                             <  2:328659/251‑1 (MQ=255)                                                                          cggcctccGCAATACTCGCTGACCGGATAACAGGTCATAACCGAGACCGGTTTGCAGATTATGCTGCCCCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAAGTTCCACCGGGCGCTGCGCCGGACGCGTCATCGGCGGTACTGGCGGCTgg                                                                                                                                                           <  2:32094/243‑1 (MQ=255)                                                                            gctcccgaaatccTCGCTCACCGGCAAACAAGTCATAACCGAGAACAGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCTCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAAGTGCCACCGGGCGCTGCACCGGACGCGTGAGCGGCGGTACTGGCGGCTggg                                                                                                                                                          <  1:405518/237‑1 (MQ=255)                                                                               ctcGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCtt                                                                                                                                                       <  1:323299/249‑1 (MQ=255)                                                                                            cTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGGAATggcgggctggcgcttcgacgatcggaa                                                                                                                                         >  1:284763/1‑228 (MQ=255)                                                                                                        aaagcaaTAACCGAGAAGGGTTTGGAGATTATGATGCACCCCCCGTCTGGGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTGTACGTTGTGCGAC‑AAAATAACCCGAAc                                                                                                                           <  2:232729/245‑1 (MQ=255)                                                                                                          aGTCATAACCGAGAACGGTGTGCAGATTATGCTGCCCCCCCCGGCTTAGCGCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAAcgc                                                                                                                         <  2:31492/251‑1 (MQ=255)                                                                                                                 cccGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCg                                                                                                                  <  1:206128/250‑1 (MQ=255)                                                                                                                          ggTTTGCATATTATGCGGCACCCCCCGGTTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCGTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGtgatga                                                                                                         <  2:430731/251‑1 (MQ=255)                                                                                                                                        gctgcCCCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTcc                                                                                           <  1:207620/251‑1 (MQ=255)                                                                                                                                          tgcACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGGTTTTTGTTGTGAGACCAAAAAAAACC‑AACGCGGCCGCggggggggtgagattatactgtcccc                                                                                         >  1:265009/1‑227 (MQ=255)                                                                                                                                                             cccGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCctgct                                                                        <  1:184799/249‑1 (MQ=255)                                                                                                                                                              ccGCCACAGCGGTTGCAGGCTGGTCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCTCCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGATCGCGGCTGCGGAGTGTTGAGCTTCATCGTCTCCAGCGTCTGATACGCCTTCTGCa                                                                     <  2:75739/251‑1 (MQ=255)                                                                                                                                                                                       gcTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCAGAAACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTc                                            <  1:172895/251‑1 (MQ=255)                                                                                                                                                                                            gACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGAc                                       <  1:347216/251‑1 (MQ=255)                                                                                                                                                                                            gACATCCGGTGTGCTTGTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTTACGCCGTGCTCCTGAc                                       <  1:346356/251‑1 (MQ=255)                                                                                                                                                                                               aTCCGGTGTGCTTTTGCTTAACGGAACAATTGAACGGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCGTCATCGTCTCCAGCGTCTGATACTCCGGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTa                                    <  2:285194/251‑1 (MQ=255)                                                                                                                                                                                                 ccGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCGGCTGCACGGTGCTGATACTGACTCCGTGCTCCTGTCTTAAg                                  <  2:431285/251‑1 (MQ=255)                                                                                                                                                                                                  cGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGc                                 <  1:138976/251‑1 (MQ=255)                                                                                                                                                                                                      gtgCTGTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGTCGCTTCCCCGGACGCGTCATCGGCGGTACGGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTGCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGGGCTGATACTGACGCCGTGCTCCTTACTTAAGCTgc                              <  2:402592/250‑1 (MQ=255)                                                                                                                                                                                                                       aaCAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTtct            <  2:125538/251‑1 (MQ=255)                                                                                                                                                                                                                                   gTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACg  <  1:254629/249‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                            ATGGCAGCCGCTGGTGAT‑GATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGAC‑AAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACG  >  NC_000913/4571041‑4571514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑