Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,494,499	C→T	intergenic (‑93/‑124)	idnD ← / → idnK	L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,494,499	0	C	T	100.0%	81.5 / NA	26	intergenic (‑93/‑124)	idnD/idnK	L‑idonate 5‑dehydrogenase/D‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/15);  total (11/15)

ATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCC  >  NC_000913/4494266‑4494742                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    ataataATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCAt                                                                                                                                                                                                                                    <  2:361334/251‑1 (MQ=255)      aatGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGt                                                                                                                                                                                                                               <  1:119272/251‑1 (MQ=255)           aaaTCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGAtgt                                                                                                                                                                                                                          >  1:287127/1‑251 (MQ=255)               cGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCt                                                                                                                                                                                                                      <  1:392082/251‑1 (MQ=255)                 gAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTg                                                                                                                                                                                                                    <  1:343245/251‑1 (MQ=255)                                                                                          cGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATg                                                                                                                                                                                                                                            <  1:165863/154‑1 (MQ=255)                                                                                          cGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATg                                                                                                                                                                                                                                            >  2:165863/1‑154 (MQ=255)                                                                                          cGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTa                                                                                                                                               >  1:240329/1‑247 (MQ=255)                                                                                           ggCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGTTAAgatg                                                                                                                                          <  1:349646/251‑1 (MQ=255)                                                                                                    tttCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGga                                                                                                                                  <  2:18937/250‑1 (MQ=255)                                                                                                    tttCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTCGCATAATTTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAACACGTGAAATTATTATGACGCCAGGCGTAGAATCGCAGCAGGTAAGATGAttacgg                                                                                                                                   >  2:19600/1‑245 (MQ=255)                                                                                                             cGCAACAACGCAGGACAGTGTTTTCCCTTGCATCAATTCCTCACTGGTCAGGAAGACCCCTCGGAAGTATTTAAGCGGTTTTAACTGTAATTATTTGTGATGAAGATAACGTCAGAAAATTGTTATATTACTCTGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAACAAACCGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCCGGAGATTTTAAAt                                                                                                                        >  2:26635/1‑251 (MQ=255)                                                                                                                     cGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGACGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAACAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGAAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTg                                                                                                                >  2:160666/1‑251 (MQ=255)                                                                                                                                     cACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGa                                                                                                 <  2:104845/250‑1 (MQ=255)                                                                                                                                      aCTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATg                                                                                               <  1:218353/251‑1 (MQ=255)                                                                                                                                                  gccTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCATAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTGTATTTTGATGGGCTTTTCAgg                                                                                    <  2:326634/249‑1 (MQ=255)                                                                                                                                                                          cGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGATATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGc                                                           <  1:287114/251‑1 (MQ=255)                                                                                                                                                                          cGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAg                                                            <  1:176107/250‑1 (MQ=255)                                                                                                                                                                            gaagaatttccgCGGTTTTAAATGTCCTTATTTGTGATGAAGATCACGTCAGAAAATTCATATATTACTATGTTACGCATAACGTGATGAGCCTCGTAATTCTTATCAGTAGAAATAAAAAACCGTGAAATTATTATGCAGCCAGGCGTAGTATAGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGAAGGTGAAAGCTGTATTTTGATGTGCGTTTCAGGGAGTTGTATAACATTAATTGGTAgaa                                                          >  2:186579/12‑248 (MQ=255)                                                                                                                                                                               gCATTTACGCGGTTTTAACTGACCTTATTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGAATAAAGTGATGTGCCTTGTACTTCTTCTCAGTCGAAATAAAACAACGTGAAATTATTATGACGCCAGGAGTAGTATAGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGAGGGTGAAAGCTTTTTTTTGATGGGCGTTTCAGGGCGTGGTAACACATTAATTGGTAGAAAggg                                                      >  2:414864/1‑250 (MQ=255)                                                                                                                                                                                 aTTTAAGCGGTTTTAACTGTCAGTAGTTGTGATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATGTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTg                                                    <  1:443603/251‑1 (MQ=255)                                                                                                                                                                                                         attTGTTATGAAGATCAAGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGTAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAAttt                             <  2:126032/250‑1 (MQ=255)                                                                                                                                                                                                            tgtgATGAAGATCACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTCTCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGAGAAAGCTTTCTTTTGATGGGCGTTTCAGGGAGTGGTAAAAAATTAATTGGTAGCAAGGTTGCCGCGTTCTTATATGCTAAATTTATTg                         >  2:452271/1‑251 (MQ=255)                                                                                                                                                                                                                      aTCACGTCAGAAAATTGTTATATTACTATGTTACGCATACCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAAAGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACg                >  2:464854/1‑250 (MQ=255)                                                                                                                                                                                                                        cACGTCAGAAAATTGTTATATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCGGCTAAATTTATTGATGGTGACGAt              <  2:120003/250‑1 (MQ=255)                                                                                                                                                                                                                                    ttGTTATATTACTATGTTACGCATAACGTGATGTGACTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAACCAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATTGTGACGATCGTCAtaaagcc  >  1:253417/1‑244 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    ATAATAATGTAAATCGGAACCGCAAATTCCACCTCGGGTTATTTGTACTAATGTTCCATTATTATTCCAATCTATCGTCTGCTCGGTAACGGCAACAGTTTTCTTGCCCGCAACAACGCAGGACTGTGTTTTCACTTGCATAAATTCCTCACTGGTCAGGTAGACACCTCGGAAGCATTTAAGCGGTTTTAACTGTCATTATTTGTGATGAAGATCACGTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGGAGATTTTAAATGGCGGGTGAAAGCTTTATTTTGATGGGCGTTTCAGGGAGTGGTAAAACATTAATTGGTAGCAAGGTTGCCGCGTTATTATCTGCTAAATTTATTGATGGTGACGATCTTCATCCAGCC  >  NC_000913/4494266‑4494742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑