Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,487,022 C→T R269C (CGT→TGT)  lptF → lipopolysaccharide transport system protein LptF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,487,0220CT98.8% 257.4 / ‑5.2 82R269C (CGT→TGT) lptFlipopolysaccharide transport system protein LptF
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (35/46);  total (35/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

GTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTT  >  NC_000913/4486779‑4487258
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            
gtggtggCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCAc                                                                                                                                                                                                                                       <  1:363579/251‑1 (MQ=255)
         gATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCATATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTAtt                                                                                                                                                                                                                              <  1:427104/251‑1 (MQ=255)
            tCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGCACTGAACTGGTGTATCACGTTGGTATTCAc                                                                                                                                                                                                                           >  2:2412/1‑251 (MQ=255)
                  cATTTAACCCAGCTTCGCGACGGCGCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTTTATGAGGTTGGTATTGACCGTGtt                                                                                                                                                                                                                     <  2:324338/251‑1 (MQ=255)
                  cATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGtt                                                                                                                                                                                                                     <  2:132408/251‑1 (MQ=255)
                           cAGCTGAGCGACGGCTCCCAGGTCGGCACTCTCAAACAGGGAACGCGCTGCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACACGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGc                                                                                                                                                                                                            >  2:61758/1‑251 (MQ=255)
                             gCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACTTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGTTATTCACCGTGTTTATGATGGCAc                                                                                                                                                                                                          <  2:396786/251‑1 (MQ=255)
                                gcgcGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTa                                                                                                                                                                                                       <  2:41994/251‑1 (MQ=255)
                                    gACGGCTCCCAGGTCGTCACTCTCCACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGt                                                                                                                                                                                                   >  2:178490/1‑251 (MQ=255)
                                    gACGGCTCACAGGTCGTCACTCTCAACCAGGGAACGCGCTTAGAAGGCACTGCAATGTTACGTGCTTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGCTCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGt                                                                                                                                                                                                   >  2:433342/1‑251 (MQ=255)
                                          tCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTAcc                                                                                                                                                                                             >  1:93914/1‑251 (MQ=255)
                                              aGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTg                                                                                                                                                                                         >  2:159983/1‑251 (MQ=255)
                                              aGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTg                                                                                                                                                                                         <  1:289663/251‑1 (MQ=255)
                                                gtcgtcACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAg                                                                                                                                                                                       <  1:452856/251‑1 (MQ=255)
                                                         ctcAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTa                                                                                                                                                                               >  1:91879/1‑250 (MQ=255)
                                                           cAACCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAAcc                                                                                                                                                                            >  1:17180/1‑251 (MQ=255)
                                                             aCCAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAAccc                                                                                                                                                                           >  1:319420/1‑250 (MQ=255)
                                                               cAGGGAACGCGCTTCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACCCCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACg                                                                                                                                                                        <  1:430075/251‑1 (MQ=255)
                                                                           ttCGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGt                                                                                                                                                            >  1:205685/1‑251 (MQ=255)
                                                                             cGAAGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTAc                                                                                                                                                          <  2:359098/251‑1 (MQ=255)
                                                                               aaGGCACTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGATCGACCCGAACGATACCGACCAGATGGCCATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTg                                                                                                                                                        >  1:392501/1‑251 (MQ=255)
                                                                               aaGGCACTGCAATGGTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTg                                                                                                                                                        <  2:214493/251‑1 (MQ=255)
                                                                                    aCTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGAt                                                                                                                                                   <  1:101276/251‑1 (MQ=255)
                                                                                    aCTGCAATGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGAt                                                                                                                                                   <  2:346297/251‑1 (MQ=255)
                                                                                      tgcaatgatACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCCCGTTGGTATTCACCGTGTTTATGATGGCCCTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGAtgc                                                                                                                                                 >  2:136384/9‑251 (MQ=255)
                                                                                      tGCAATGTTACGTGCTTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGAAACCGCTCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGAGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGAACTGTAGAtgc                                                                                                                                                 >  2:419740/1‑251 (MQ=255)
                                                                                        caatGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGGCATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGAtgctg                                                                                                                                               <  1:193184/248‑1 (MQ=255)
                                                                                              tACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTgccagc                                                                                                                                          <  2:26729/250‑1 (MQ=255)
                                                                                              tACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCAATGGTCACCAGGCGGTGGCGCTCGACCCGAACGATTCCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTgccagc                                                                                                                                          <  2:93914/250‑1 (MQ=255)
                                                                                               aCGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTgccagccc                                                                                                                                        >  2:349092/1‑250 (MQ=255)
                                                                                               aCGTGATTTCCGCATTACGGACTACCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCATATGGACATGCGCACATTGTGGAACACTGACACCGAGCGTGCTCGCGCAGATCTGAACTGGTGTATCACGTTGCTAGTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGGCAGGTACGCGTACTGGCGATGCTgccagcc                                                                                                                                         <  2:83510/250‑1 (MQ=255)
                                                                                                 gTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCAtg                                                                                                                                      <  1:214283/251‑1 (MQ=255)
                                                                                                   gATTTCCGCATTACGGACTTCCAGGATTATCATGCGATCATTGTTCACCAGGCGGTTGCGCTCGACCCGAACGATACCGACTAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCTCGTTGGTATTCACCGTGTTTATGGTTGCACTTATGGGCGTACCGCTGAGCGTGGTTAGCCCACGTCTGGGACGCGTGCTGTCGATGCTGCCAGCCAtgc                                                                                                                                     <  2:408882/250‑1 (MQ=255)
                                                                                                         cGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGt                                                                                                                               >  1:287785/1‑250 (MQ=255)
                                                                                                           cATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCGGCCAGCCATGCTGCTGTATc                                                                                                                            <  2:132750/251‑1 (MQ=255)
                                                                                                            aTTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCt                                                                                                                           <  1:420617/251‑1 (MQ=255)
                                                                                                              tACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGCTGGCACTTATGGTCGTAACGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTAc                                                                                                                         >  2:355214/1‑251 (MQ=255)
                                                                                                                    cTTCCAGGATTATCAGGCGATCATTGGACACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACCCTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCCTGCTGCTGTATCTACTTttct                                                                                                                   >  2:450037/1‑251 (MQ=255)
                                                                                                                       ccAGGATTATCAGGCGAGCATTGGTCACCAGGCGGTTGCGCTCGACCCGACCGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTTGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTcc                                                                                                                <  1:461837/251‑1 (MQ=255)
                                                                                                                           gATTATCAGGCGCTCCTTGGTCACCAGGCGGTGGCGCTCGACCCGAACGGTACCGACAAGATGGACCTGCGAACATTGTGGACCACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCCCGTTGGTATTCACCGTGTTTATGATGGAACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTTTATCTACTTTTCTTACTgct                                                                                                            >  2:312492/1‑249 (MQ=255)
                                                                                                                           gATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTCCTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGAt                                                                                                            >  2:306358/1‑251 (MQ=255)
                                                                                                                            aTTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATTGTCGGACCGCTGAGCGTGGTTAACCCACGTCAGGGAGGCGTACTGTCTATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTGCCTGATc                                                                                                           <  2:413107/251‑1 (MQ=255)
                                                                                                                                tCAGGCGATCATTAGTCACCAGGCGGTGGCGCTCGCCCCGAACGATACCGACCAGATGGACCTGCGCACATTGTGGCCCACTGACACCGATCGTGCTCGCGCAGCACTGAACTGGTGTATCACGTTGGTATTCCCCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGACAGCCATGCTGCTGTATCTACTTTTCTTCCTGATcc                                                                                                          >  2:231329/1‑248 (MQ=255)
                                                                                                                                    gCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGCACGATACCGACCAGATGGACATGCGCACATTGTGAAACACTGAAACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACTTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGACAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGAcatc                                                                                                   >  1:382620/1‑248 (MQ=255)
                                                                                                                                    gCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTc                                                                                                   >  1:151127/1‑251 (MQ=255)
                                                                                                                                         cATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTg                                                                                               >  2:31264/1‑250 (MQ=255)
                                                                                                                                         cATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATAACGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGATCTCCCTg                                                                                               <  1:354023/250‑1 (MQ=255)
                                                                                                                                             ggTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCGCGCGCAGAACTGAACTGGTGTAGCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGTTCGTACCGCTGAGCGTGGTTAACCCACGTCAGTGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATc                                                                                          <  2:392002/251‑1 (MQ=255)
                                                                                                                                                  ccAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAAcg                                                                                     <  2:205685/251‑1 (MQ=255)
                                                                                                                                                   cAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACGGACACCGAGCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTGATGGGCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAAcgg                                                                                    <  1:395197/251‑1 (MQ=255)
                                                                                                                                                        ggTGGCGCTCGACCCGCACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCGGAACTGAACTGGTGTATCACGTCGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCTCGTCAGGGCCGCGTACTGTCGATGCTGCCAGCCATCCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAACTCGAAcagcggg                                                                                >  2:148912/1‑249 (MQ=255)
                                                                                                                                                         gTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTaa                                                                              <  1:142359/251‑1 (MQ=255)
                                                                                                                                                          tGGCGCTAGACCCGAACGATACTGACCAGATGGACATGCGCCCATTGTGCAACACTGAAACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGAGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTCTCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTaa                                                                              >  2:419664/1‑250 (MQ=255)
                                                                                                                                                                 cGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAg                                                                       <  1:2412/250‑1 (MQ=255)
                                                                                                                                                                  gACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCt                                                                     >  1:255011/1‑251 (MQ=255)
                                                                                                                                                                    cccGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTgg                                                                   >  1:294066/1‑251 (MQ=255)
                                                                                                                                                                    cccGAACGATACCGACCAGATGGACATGCGCACATCGTGGAACACTGACACCGAGCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTTGTATTCACCGTGTTGATGATGGCACTTATGGTCGTACCGCTGAGCGTGTTTAACCCACGTCAGTGACGTGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTGCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTgg                                                                   <  2:162090/251‑1 (MQ=255)
                                                                                                                                                                    cccGAACGATACCGACCAGATGGACATGCGAACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGAATTCACCGTGTTTATGATGGCACTTATGATCGTCCCGCTGAGCGTGGTTATCCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCCTGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCATGAAATCGAACGGCGGTaaaagttaactgg                                                                   >  2:219712/1‑241 (MQ=255)
                                                                                                                                                                        aaCGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTGTGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGAccc                                                               <  1:75548/251‑1 (MQ=255)
                                                                                                                                                                           gATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGAc                                                            <  1:31264/251‑1 (MQ=255)
                                                                                                                                                                                  aCCAGATGGACAAGCGCACATTGTGGAACACTGACACCTATCGTGCTCGCGCAGAACTGATCTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTAGGGTCGTTCCGCTGAGCGTGGTTAACCCACGGCAGGGACGCGTACTGTCGATGCTGCCAGCCAGGCTTCTGTATCTTCTTGTCTTACGGATCCAGACCTCCCTTAAATCGAACGGCGGTAAAGGTAAGCTGGACCCTACGCtgtgg                                                     <  2:434459/251‑1 (MQ=255)
                                                                                                                                                                                           aCATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGAcc                                            <  1:412895/251‑1 (MQ=255)
                                                                                                                                                                                               gcgcACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCATCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTa                                        <  1:466005/251‑1 (MQ=255)
                                                                                                                                                                                                cgcACATTGTGGAACACTGACTCCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATGCACCGTGTTTATGATGGCACTTAGGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTTCCAGCCATGCTGCTGTATCttcgttgct                                                                                                                   <  2:457892/175‑9 (MQ=255)
                                                                                                                                                                                                cgcACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGCTGCTGCCAGCCATGCTGCTGTATCTACTTttct                                                                                                                   >  1:457892/1‑175 (MQ=255)
                                                                                                                                                                                                    caTTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCCCCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTg                                   <  1:263495/251‑1 (MQ=255)
                                                                                                                                                                                                          ggaacccttaccccGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGCCCCGACGCTGTGGATGTGGACCGTTAACCTGATTTAt                             <  2:105479/239‑1 (MQ=255)
                                                                                                                                                                                                              cacTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTTTATCACTTTGGTATTCCCCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTgg                         <  2:344959/251‑1 (MQ=255)
                                                                                                                                                                                                              cacTGACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTgg                         <  1:159983/251‑1 (MQ=255)
                                                                                                                                                                                                                  gACACCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCttt                     >  2:116651/1‑251 (MQ=255)
                                                                                                                                                                                                                   acacCGATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCtttt                    >  1:215062/1‑250 (MQ=255)
                                                                                                                                                                                                                        gATCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGAGCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGAt               <  1:107003/251‑1 (MQ=255)
                                                                                                                                                                                                                         aTCGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCCTGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGGCCCGACGCTGGGGGTGTTGAGCGGTAACATGATTTATCTTGCTTTAGCgctt              >  2:116425/1‑248 (MQ=255)
                                                                                                                                                                                                                           cGTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGGGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTg             <  2:17180/250‑1 (MQ=255)
                                                                                                                                                                                                                           cGTGCTCGCGCAGAACGGAACTGGTGTATCCCGTTGGGTGTCAACGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGCCGCGTACTGTCGATGCTGTCAGCCATGCTGCTGTAGCTACTTTTCTTCCTGATCTAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTTGATGTGGACCGTTAACCTGATGTATCTGGCTTTCGCGATTGt            <  2:208114/251‑1 (MQ=255)
                                                                                                                                                                                                                            gTGCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGtt           <  2:151127/251‑1 (MQ=255)
                                                                                                                                                                                                                              gtgcgcgCAGACCTGATCTGGTGTGTCTCGTTGGTCTTTCCCGGGTTTATTATGGCACGTTTGGTAGTACCGCGGAGCGTGGTGAACCTACGTCAGGGAAGCGTACTGTCGATGCTACCAGCCATGCTGCTGTATCTACTTTTCGTCCTGATCCAGACCTCATTGAAATCGAACGTCGGTAAAGGTAAGCTGGAATCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTTTtct         <  2:359731/248‑1 (MQ=255)
                                                                                                                                                                                                                              gCTCGCGCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGAAGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTtct         >  1:388797/1‑251 (MQ=255)
                                                                                                                                                                                                                               cgcgcgCAGAACTGAACTGGTGTATCACGTTGGTATTCACCGGTTTTATTATGGCACTTATGTTCGTACCGCTTAGCGTGGTTAACCCACGTCAGGGACGCGTACGGTCGAGGCTGCCAGCCATGCGGCTGTATCTGCTTTTCTGCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCGGTGGATGTGGACCGTTATCCTGAGTTATCTGGCTTTAGCGATTGTtct         <  2:392501/248‑1 (MQ=255)
                                                                                                                                                                                                                                    gcAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAAACTGATTTATCTGGCTTTAGCGATTGTTCTCAACCt   >  1:373777/1‑251 (MQ=255)
                                                                                                                                                                                                                                     cAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGGCGCTGTGGATGTGGACCGGTAACCTGGTTTATCTgggtaggttgggagggcgtcgggtg  >  2:184568/1‑228 (MQ=255)
                                                                                                                                                                                                                                     cAGAACTGAACTGGTGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTGCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCt                       <  1:184568/230‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            
GTGGTGGCCGATTCCGGACATTTAACCCAGCTGCGCGACGGCTCCCAGGTCGTCACTCTCAACCAGGGAACGCGCTTCGAAGGCACTGCATTGTTACGTGATTTCCGCATTACGGACTTCCAGGATTATCAGGCGATCATTGGTCACCAGGCGGTGGCGCTCGACCCGAACGATACCGACCAGATGGACATGCGCACATTGTGGAACACTGACACCGATCGTGCTCGCGCAGAACTGAACTGGCGTATCACGTTGGTATTCACCGTGTTTATGATGGCACTTATGGTCGTACCGCTGAGCGTGGTTAACCCACGTCAGGGACGCGTACTGTCGATGCTGCCAGCCATGCTGCTGTATCTACTTTTCTTCCTGATCCAGACCTCCCTGAAATCGAACGGCGGTAAAGGTAAGCTGGACCCGACGCTGTGGATGTGGACCGTTAACCTGATTTATCTGGCTTTAGCGATTGTTCTCAACCTT  >  NC_000913/4486779‑4487258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑