Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,482,901	C→T	V313M (GTG→ATG)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,482,901	0	C	T	98.5%	220.5 / NA	66	V313M (GTG→ATG)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (36/29);  minor base G (0/1);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGT  >  NC_000913/4482659‑4483147                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        cgccgGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGgcgc                                                                                                                                                                                                                                                 >  2:138262/1‑251 (MQ=255)                gACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTACGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGc                                                                                                                                                                                                                                  >  1:278861/1‑251 (MQ=255)                 aCATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACTGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGcc                                                                                                                                                                                                                                 <  1:374463/251‑1 (MQ=255)                                tACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGAt                                                                                                                                                                                                                  >  1:385586/1‑251 (MQ=255)                                 aCCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATg                                                                                                                                                                                                                 <  1:42765/251‑1 (MQ=255)                                     cTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTg                                                                                                                                                                                                              >  1:127617/1‑250 (MQ=255)                                         tcggtcAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGATTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCCACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGCTATCGCCGTCAAAGGTCAGGATGTTGatcat                                                                                                                                                                                                         >  2:122295/1‑251 (MQ=255)                                          cggtcAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTCTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGatcat                                                                                                                                                                                                         <  1:458037/250‑1 (MQ=255)                                                        tcgaaaccTACGCCTCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCTGGGCGTGAc                                                                                                                                                                                           <  2:272463/243‑1 (MQ=255)                                                        tCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACg                                                                                                                                                                                          >  2:217672/1‑251 (MQ=255)                                                         cGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTAGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACg                                                                                                                                                                                          <  1:131009/250‑1 (MQ=255)                                                             aaCTACGCCGCCACGGTCGTCGGAAGGAACGGTCAGGTCGGGCGGTTTAATTTCTGCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTAc                                                                                                                                                                                     <  1:4336/251‑1 (MQ=255)                                                              acgaCGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGGCAACTGCGGCAACGGCTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCATGATGTTGATCATCGGCAGGGCGTGACGTTTAcc                                                                                                                                                                                    <  1:259214/248‑1 (MQ=255)                                                               cTACGCAGCCACGGTCGACGTCAGGAACGGACAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGATTGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCCAAGGTCAGGATGTTGATCATCGGCAGAGCgggtagttttccc                                                                                                                                                                                   >  2:431889/1‑239 (MQ=255)                                                                 aCGCCGCCACGGGCGCCGTAAGGAACGGGCAGGTCGTTCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTGTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCAc                                                                                                                                                                                 <  2:348603/251‑1 (MQ=255)                                                                       ccACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATa                                                                                                                                                                           <  1:86258/251‑1 (MQ=255)                                                                        cACGGTCGCCGTAAGGAACGGTCAGGTCGTTCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACGCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCGTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAg                                                                                                                                                                          <  2:223131/251‑1 (MQ=255)                                                                                 cGTAAGGCATGGTCGGGTAGTGGGGTTTAATTACTACCAGCAGGCCAAGCGCGTCAACTCCGGAAACGACTGATTTACGTGCAGCAAAACGCTCCCGTTTCTGGAACCCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCAGGGCGCTTTCACGGATATCGCCGTCAACGGTCAGGATGTTGATCATCGGCAGGACGTGACGGTTAACAACATAATCGTCGTTaaa                                                                                                                                                                  >  2:281874/1‑250 (MQ=255)                                                                                     aGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGACGAGACGTTTACCCACTTCATCGTCGTTAAAGTCGt                                                                                                                                                             >  1:302879/1‑251 (MQ=255)                                                                                        aaCGGTCAGGTCGTGCGGTTTAATTTCTTCAAGCAGGCCAAGCGCGTAAACAGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGATTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGCTCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTgcg                                                                                                                                                          >  1:182136/1‑251 (MQ=255)                                                                                              cAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCgg                                                                                                                                                      >  1:422999/1‑249 (MQ=255)                                                                                              cAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCgg                                                                                                                                                      >  1:47001/1‑249 (MQ=255)                                                                                                ggTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGt                                                                                                                                                             <  1:242549/240‑1 (MQ=255)                                                                                                ggTCGTGCGGTTTAATTACTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAAAGACTGCTTTACGTGCAGCAACACGCTCCAGTTTCTGGAAATCTGCAGGGATTTCGCTGGAATAAACGTCAGCTTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAACGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCCCTTCCTAGTCGTTAAAGTCGt                                                                                                                                                             >  2:242549/1‑240 (MQ=255)                                                                                                    gTGCGGTTTTATTTCTTACACCAGGCCCCGCGCGCCAACTGCGTCACGGACTGCTTATCGTCAAGCAACACGCTACAGTTTATGGACCCACGCAGGGAATTCGCTGGAATAACCGTCAGATTCGTTACCTTTCGTATCGAACCTCCGGGCGCTTTCACGGCTATCGCCGCCAAAGGTCAGGATGTTGATCCTCGGAAGGGCGCGCTGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGAttt                                                                                                                                              >  2:443893/1‑251 (MQ=255)                                                                                                    gTGCGGTTTAATTTCTTCAAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGAAGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGAttt                                                                                                                                              >  2:324328/1‑251 (MQ=255)                                                                                                            tAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGc                                                                                                                                        >  1:139837/1‑249 (MQ=255)                                                                                                             aaTTTCTGCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGGCAGATTCGTTTCCTTTGGTATCGAACATCGGTGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGAGCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTCAAAGTCGTGCGCCGGAGTGATTTTCACGCAg                                                                                                                                      <  1:420499/250‑1 (MQ=255)                                                                                                                   ttcCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAATATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGc                                                                                                                               >  1:166657/1‑251 (MQ=255)                                                                                                                         cagGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCttttt                                                                                                                         >  2:31793/1‑251 (MQ=255)                                                                                                                            gCCAAGAGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAACCGTAAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAACGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTca                                                                                                                       >  1:33487/1‑249 (MQ=255)                                                                                                                              cAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCACAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCAtt                                                                                                                    >  2:147938/1‑250 (MQ=255)                                                                                                                                        aaCTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGtt                                                                                                          >  2:73983/1‑251 (MQ=255)                                                                                                                                           tGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTtcgt                                                                                                       >  1:70245/1‑251 (MQ=255)                                                                                                                                              ggCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCCGGGATTTCGCTGGAATAAACGTCAGATTCGTTAACTTTGGTATCGAACATCTGGGAGCTTTCACGGATATCGCCGTCAAAGGTCCGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCCTAGTCgct                                                                                                                                                                     >  1:21496/1‑184 (MQ=255)                                                                                                                                              ggCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAAGAAACGGCAGATTCGTTACCTTTGGTATCGAACAGCTGGGCGCTTTCACGGAGATCGCCGTCAAAGGTCAGGAGGTTGATCATCGGCAGGGCGTGACGTTTACCCACTGCATAGTCGTTAAAGTCGTGCGCCGGAGGTATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGc                                                                                                    <  1:73682/251‑1 (MQ=255)                                                                                                                                              ggCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACGCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTAGCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGGGACGTGTACCCTCTTCATAGTCGtt                                                                                                                                                                     <  2:21496/186‑1 (MQ=255)                                                                                                                                                cAACGTCTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGACCTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGGTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCTGCGTGGTCGTCGCCa                                                                                                  <  2:266685/251‑1 (MQ=255)                                                                                                                                                  aCGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCGTTTTCCATGTCGGCGTGTTCGTCGCCAAc                                                                                                <  1:122295/251‑1 (MQ=255)                                                                                                                                                           tgaCGTGCAGCAAAACGCTCCATTGTTTGGGACTCTTCATTGATTCCGCTGGGATAAACGTCAGATTCGTTACCTTTGGTATCGAACATATGGGCGCGTTCACGGATATCTCCGCCAAAGGTCAGGATGTTGAGCATCGGCAGGGCGGGACGTTTACCCCCTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGGCGGCGTGTTCGTCGTCAACGATCGGa                                                                                         <  2:339590/247‑1 (MQ=255)                                                                                                                                                             aCGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATa                                                                                      <  1:460570/250‑1 (MQ=255)                                                                                                                                                              cGTGCAGCAAAACGCTCCAGTCTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCAAGGATATCGCCGTCACAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACACACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATacg                                                                                    >  1:307566/1‑251 (MQ=255)                                                                                                                                                                      aaaaCGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑a                                                                            >  1:104981/1‑250 (MQ=255)                                                                                                                                                                          cGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCAATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AAcc                                                                         >  1:38063/1‑249 (MQ=255)                                                                                                                                                                              ccattgtcgggAACTCTGCAGGGATTTCGCGGGAATAAACGTCAGATTCGTTACCTGTGGTATCTAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCTCTTCATAGTCGGTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCgg                                                                    <  2:166170/241‑1 (MQ=255)                                                                                                                                                                                agtatCTGGAACTCGGAAGGGAGTTCGCGGGAAGAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTCGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCACCGGCAGGGAGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCGCTTTCCATGCCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACAAGCGGCAg                                                                 <  2:140903/247‑1 (MQ=255)                                                                                                                                                                                 gTTTCTGGAACTCTGCCGGGATATCGCTGGAATAAACGTCAGACTCGTTACCTTTGGTATCGAACATCTGGGCGCTTGCACGTAGATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTGCATAGTCTTTAAAGTCTTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCGTTTTCCATGTCGGCGTGTTCGTCGCCAACGTTCTGAATACGACTGTT‑AACCTGCGGCAGa                                                                <  2:380511/251‑1 (MQ=255)                                                                                                                                                                                   ttCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAAt                                                              >  2:379859/1‑251 (MQ=255)                                                                                                                                                                                            tctGCAGGGATTTCGCTGGAATAACCGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAATGACATAttt                                                     >  2:151033/1‑251 (MQ=255)                                                                                                                                                                                               gCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGGCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAATGACATATTTGcc                                                  >  1:15989/1‑251 (MQ=255)                                                                                                                                                                                                 aGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGCTTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGCCGGTT‑AACCAGCGGCAGAATGACATATTTGCCa                                                 >  2:184170/1‑250 (MQ=255)                                                                                                                                                                                                    gATTTCGCTGGAATAAACGTCAGATTCGTTTCCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAATGACATATTTGCCAATCa                                             <  2:208897/251‑1 (MQ=255)                                                                                                                                                                                                         cGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAATGACATATTTGCCAATCAGATCt                                        <  1:156782/251‑1 (MQ=255)                                                                                                                                                                                                               aaTAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGCCGGTT‑AACCAGCGGCAGAATGACat                                                         >  1:262791/1‑228 (MQ=255)                                                                                                                                                                                                               aaTAAACGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATAGCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGGCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAATGACat                                                         <  2:262791/228‑1 (MQ=255)                                                                                                                                                                                                                 tAAACGCCAGATTCGTTACATTTGGTATCGAACATCTCGGCGCTTTAACGGATATCGCCGTCAAAGGTCCGGATGTTGATCCTCGGCAGGCCGAGCCGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCATTTTTCCATGTCGGCGTGTTCGTCGCCAAAGCTCGGAATACGACGGTTCAACC‑GCGGCAGACTGAAATCTTTGACAATCAGATCTTTGTAAcg                                >  2:414657/1‑251 (MQ=255)                                                                                                                                                                                                                   aaCGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAAcgcg                              <  1:257563/251‑1 (MQ=255)                                                                                                                                                                                                                   aaCGTCAGAGTCGGTACCTTTGGTAACGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTGCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAAtgcg                              <  2:335824/251‑1 (MQ=255)                                                                                                                                                                                                                     cGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCgt                                                                                                             >  2:354645/1‑171 (MQ=255)                                                                                                                                                                                                                     cGTCAGATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCgt                                                                                                             <  1:354645/171‑1 (MQ=255)                                                                                                                                                                                                                          gATTCGTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTc                       <  1:73983/251‑1 (MQ=255)                                                                                                                                                                                                                               gTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCgg                                                                    <  1:164527/201‑1 (MQ=255)                                                                                                                                                                                                                               gTTACCTTTGGTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCgg                                                                    >  2:164527/1‑201 (MQ=255)                                                                                                                                                                                                                                        ggTATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCt          <  1:217672/250‑1 (MQ=255)                                                                                                                                                                                                                                          tATCGAACATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTACAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACg       >  1:415885/1‑251 (MQ=255)                                                                                                                                                                                                                                               aaCATCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGAGCGTGACGTTTACCCCCTTCATAGTCGTTAAAATCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGCATACGACGGTT‑AACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCTGATCTTCAGGGTTAACGGCTCCGCCAGt  >  1:280995/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                        CGCCGGTTTCGCCAGGACATCGGCACGCACGTACCACTGGTCGGTCAGCATTGGTTCGATAACTACGCCGCCACGGTCGCCGTAAGGAACGGTCAGGTCGTGCGGTTTAATTTCTTCCAGCAGGCCAAGCGCGTCAACTGCGGCAACGACTGCTTTACGTGCAGCAAAACGCTCCAGTTTCTGGAACTCTGCAGGGATTTCGCTGGAATAAACGTCAGATTCGTTACCTTTGGTATCGAACACCTGGGCGCTTTCACGGATATCGCCGTCAAAGGTCAGGATGTTGATCATCGGCAGGGCGTGACGTTTACCCACTTCATAGTCGTTAAAGTCGTGCGCCGGAGTGATTTTCACGCAGCCGGTGCCTTTTTCCATGTCGGCGTGTTCGTCGCCAACGATCGGAATACGACGGTT‑AACCAGCGGCAGAATGACATATTTGCCAATCAGATCTTTGTAACGCGGATCTTCCGGGTTAACGGCTACGCCAGT  >  NC_000913/4482659‑4483147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑